medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 26, 2024
Abstract We assessed concentrations of parvovirus B19 DNA from two wastewater treatment plants in a county with known outbreak 2024. Wastewater viral correlated significantly clinical cases, demonstrating wastewater’s potential for tracking infections. Peaks were aligned the peak hydrops fetalis diagnoses.
Language: Английский
medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Abstract In 2024, the highly pathogenic avian influenza A H5N1 caused outbreaks in wild birds, poultry, cows, and other mammals United States with 61 human cases also reported by CDC. Detection of H5 RNA wastewater has been previously sewersheds Texas North Carolina nearby impacted dairy herds following emergence cows. Here, we conduct retrospective testing total hemagglutinin genes as well presenting applying new assays for detection H1 H3 across a respiratory virus season an urban California sewershed from September 2023 – May 2024. Total A, H1, were regularly detected, while was first detected March. We developed model that uses Monte Carlo simulations published parameters to estimate numbers infected people, or liters H5-contaminated milk required result measured concentrations wastewater. Our findings demonstrate this sewershed, contaminated poultry most likely sources created publicly available tool apply input inputs. Synopsis understand potential wastewater, enabling interpretation detections. TOC figure
Language: Английский
Citations
2
Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)
Published: Oct. 17, 2024
We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A B viruses, syncytial virus, human metapneumovirus, enterovirus D68, parainfluenza types 1, 2, 3, 4a, 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis adenovirus, mpox H5 pepper mild mottle virus nucleic acids wastewater solids prospectively at 191 treatment plants 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 30 June 2024, depending on plant staff availability. using droplet digital (reverse-transcription-) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used better understand disease occurrence communities contributing wastewater.
Language: Английский
Citations
6
ACS ES&T Water, Journal Year: 2025, Volume and Issue: 5(2), P. 985 - 992
Published: Jan. 22, 2025
Wastewater measurements represent an entire contributing population and can be available within 24 h. Enhanced information about disease occurrence improve health through better timing of policies interventions. We aimed to infer seasonal patterns for common respiratory viruses alongside transmission dynamics SARS-CoV-2 across the USA using wastewater samples. used RNA concentrations influenza A B (IAV/IBV), syncytial virus (RSV), human metapneumovirus (HMPV), from 175 treatment plants (July 2023–June 2024). For IAV, IBV, RSV, HMPV, we determined epidemic onset, offset, peak, duration at national subnational scales. SARS-CoV-2, categorized based on recent levels trends. Epidemic onset occurred in November IAV RSV which aligned with prepandemic norms. Onset January IBV April HMPV were later than expected according historical data. Duration was longer shorter peak dates consistent norms all viruses. Peak coincided high, upward trending concentrations, suggesting potential co-occurrence these
Language: Английский
Citations
0
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0313927 - e0313927
Published: Nov. 18, 2024
Wastewater-Based Epidemiology (WBE) has become a powerful tool for assessing disease occurrence in communities. This study investigates the coronavirus 2019 (COVID-19) epidemic United States during 2023–2024 using wastewater data from 189 treatment plants 40 states and District of Columbia. Severe acute respiratory syndrome 2 (SARS-CoV-2) pepper-mild mottle virus normalized SARS-CoV-2 RNA concentration were compared with COVID-19 hospitalization admission at both national state levels. We further investigate temporal features viral abundance, peak timing cross-correlation lag analyses indicating that concentrations precede admissions by to 12 days. Lastly, we demonstrate plant size significant effect on variability measured concentrations. highlights effectiveness WBE as non-invasive, timely resource-efficient monitoring strategy, especially context declining clinical reporting.
Language: Английский
Citations
2
ACS ES&T Water, Journal Year: 2024, Volume and Issue: 5(1), P. 341 - 350
Published: Dec. 16, 2024
Since the start of coronavirus-19 pandemic, use wastewater-based epidemiology (WBE) for disease surveillance has increased throughout world. Because wastewater measurements are affected by external factors, processing WBE data typically includes a normalization step in order to adjust (e.g., viral ribonucleic acid (RNA) concentrations) account variation due dynamic population changes, sewer travel effects, or laboratory methods. Pepper mild mottle virus (PMMoV), plant RNA abundant human feces and wastewater, been used as fecal contamination indicator normalize extensively. However, there little work characterize spatiotemporal variability PMMoV which may influence effectiveness adjusting normalizing measurements. Here, we investigate its across space time using collected over two-year period from sewage treatment plants United States. We find that most can be attributed longitude latitude followed site-specific variables. Further research into cross-geographical -temporal comparability PMMoV-normalized pathogen concentrations would strengthen utility WBE.
Language: Английский
Citations
0
medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 26, 2024
Abstract We assessed concentrations of parvovirus B19 DNA from two wastewater treatment plants in a county with known outbreak 2024. Wastewater viral correlated significantly clinical cases, demonstrating wastewater’s potential for tracking infections. Peaks were aligned the peak hydrops fetalis diagnoses.
Language: Английский
Citations
0