Identification and expression of detoxification genes provide insights into host adaptation of the walnut pest Atrijuglans aristata DOI Creative Commons
Dandan Feng, Cai‐qing Yang, Michael C. Orr

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 18, 2025

Despite the presence of a large number toxic components, primarily juglone, in walnut green husks, these components have failed to prevent infestations specialized pest Atrijuglans aristata. At present, it remains unclear whether detoxification genes play pivotal role enhancing host fitness A. In this study, we explored adaptation mechanisms aristata plants by identifying and expressing gene families associated with detoxification, as well assessing binding affinity their protein products juglone. We identified 84 P450 (P450 monooxygenases), 48 COE (carboxylesterases), 34 GST (glutathione S-transferases), 26 UGT (UDP-glycosyltransferases), 57 ABC (ATP-binding cassette) transporter genome The family was divided into four classes based on phylogenetic relationships. Comparative transcriptome analysis revealed that 383 larval guts were significantly down-regulated after starvation treatment compared normal feeding. These frequently enriched pathways related metabolism. Through homology modeling molecular docking 12 genes, found all proteins exhibited strong affinities ligand molecule detoxification-related is close other insect species. Twelve candidate comparative are inferred be involved results provide theoretical basis for management pests.

Language: Английский

Identification and expression of detoxification genes provide insights into host adaptation of the walnut pest Atrijuglans aristata DOI Creative Commons
Dandan Feng, Cai‐qing Yang, Michael C. Orr

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 18, 2025

Despite the presence of a large number toxic components, primarily juglone, in walnut green husks, these components have failed to prevent infestations specialized pest Atrijuglans aristata. At present, it remains unclear whether detoxification genes play pivotal role enhancing host fitness A. In this study, we explored adaptation mechanisms aristata plants by identifying and expressing gene families associated with detoxification, as well assessing binding affinity their protein products juglone. We identified 84 P450 (P450 monooxygenases), 48 COE (carboxylesterases), 34 GST (glutathione S-transferases), 26 UGT (UDP-glycosyltransferases), 57 ABC (ATP-binding cassette) transporter genome The family was divided into four classes based on phylogenetic relationships. Comparative transcriptome analysis revealed that 383 larval guts were significantly down-regulated after starvation treatment compared normal feeding. These frequently enriched pathways related metabolism. Through homology modeling molecular docking 12 genes, found all proteins exhibited strong affinities ligand molecule detoxification-related is close other insect species. Twelve candidate comparative are inferred be involved results provide theoretical basis for management pests.

Language: Английский

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