Proteomic and metabolomic insights into the impact of topping treatment on cigar tobacco DOI Creative Commons

Dong Guo,

Huajun Gao,

Tongjing Yan

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 20, 2025

Top removal is a widely utilized method in production process of tobacco, but little known regarding the way it impacts protein and metabolic regulation. In this study, we investigated underlying processes alterations cigar tobacco leaves with without top removal, using combined proteomic metabolomic approach. The results revealed that: (1) Topping significantly affected superoxide anion (O2 -) levels, dismutase (SOD) activity, malondialdehyde (MDA) content, (2) proteome, 385 differentially expressed proteins (DEPs) were identified, 228 upregulated 156 downregulated. Key pathways enriched included flavonoid biosynthesis, porphyrin chlorophyll metabolism, cysteine methionine amino acid biosynthesis metabolism. A network 161 nodes interconnected by 102 altered was established, (3) metabolome, 247 different metabolites (DEMs) 120 128 downregulated metabolites, mainly comprising lipids lipid-like molecules, phenylpropanoids polyketides, organic acids derivatives, heterocyclic compounds, (4) KEGG pathway enrichment upregulation such as chalcone synthase (CHS), isomerase (CHI), naringenin 3-dioxygenase (F3H), 3'-monooxygenase (F3'H), along like pinocembrin, kaempferol, trifolin, rutin, quercetin, enhancing 'flavonoid' 'flavone flavonol' biosynthesis. This study sheds light on responses after topping.

Language: Английский

Phytochemical Properties, Extraction, and Pharmacological Benefits of Naringin: A Review DOI Creative Commons

VS Shilpa,

Rafeeya Shams,

Kshirod Kumar Dash

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(15), P. 5623 - 5623

Published: July 25, 2023

This review describes the various innovative approaches implemented for naringin extraction as well recent developments in field. Naringin was assessed terms of its structure, chemical composition, and potential food sources. How works pharmacologically discussed, including an anti-diabetic, anti-inflammatory, hepatoprotective substance. Citrus flavonoids are crucial herbal additives that have a huge spectrum organic activities. is nutritional flavanone glycoside has been shown to be effective treatment few chronic disorders associated with ageing. fruits contain common flavone specific pharmacological biological properties. Naringin, range intriguing characteristics, abundant citrus fruits. variety biological, medicinal, effects. hydrolyzed into rhamnose prunin by naringinase, which also possesses l-rhamnosidase activity. D-glucosidase subsequently catalyzes hydrolysis glucose naringenin. known having antioxidant, tumor-fighting Numerous test animals cell lines used correlate exposure asthma, hyperlipidemia, diabetes, cancer, hyperthyroidism, osteoporosis. study focused on many documented actions in-vitro in-vivo experimental preclinical investigations, prospective therapeutic advantages, utilizing information presently accessible literature. In addition pharmacokinetic naringin’s distribution, different methods, use cosmetic, food, pharmaceutical, animal feed sectors were discussed.

Language: Английский

Citations

75

Functional Endophytes Regulating Plant Secondary Metabolism: Current Status, Prospects and Applications DOI Open Access

Zhaogao Li,

Keyi Xiong,

Weie Wen

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 1153 - 1153

Published: Jan. 6, 2023

Endophytes, which are widely found in host plants and have no harmful effects, a vital biological resource. Plant endophytes promote plant growth enhance plants’ resistance to diseases, pests, environmental stresses. In addition, they the synthesis of important secondary metabolites improve potential applicability agriculture, medicine, food, horticulture. this review, we summarize recent progress understanding interaction between construction synthetic microbial communities (SynComs) metaomics analysis plants. The application development prospects other industries also discussed provide reference for further study utilization endophytes.

Language: Английский

Citations

55

Citrus Flavones: An Update on Sources, Biological Functions, and Health Promoting Properties DOI Creative Commons
Davide Barreca, Giuseppina Mandalari,

Antonella Calderaro

et al.

Plants, Journal Year: 2020, Volume and Issue: 9(3), P. 288 - 288

Published: Feb. 26, 2020

Citrus spp. are among the most widespread plants cultivated worldwide and every year millions of tons fruit, juices, or processed compounds produced consumed, representing one main sources nutrients in human diet. Among these, flavonoids play a key role providing wide range health beneficial effects. Apigenin, diosmetin, luteolin, acacetin, chrysoeriol, their respective glycosides, that occur concentrations up to 60 mg/L, common flavones found fruits juices. The unique characteristics basic skeleton nature position substituents have attracted stimulated vigorous investigations as consequence an enormous biological potential, manifests itself (among other properties) antioxidant, anti-inflammatory, antiviral, antimicrobial, anticancer activities. This review analyzes biochemical, pharmacological, properties flavones, emphasizing occurrence on bioavailability, ability modulate signal cascades metabolic enzymes both vitro vivo. Electronic databases including PubMed, Scopus, Web Science, SciFinder were used investigate recent published articles terms components bioactivity potentials.

Language: Английский

Citations

115

CsCYT75B1, a Citrus CYTOCHROME P450 Gene, Is Involved in Accumulation of Antioxidant Flavonoids and Induces Drought Tolerance in Transgenic Arabidopsis DOI Creative Commons
Muhammad Junaid Rao,

Yuantao Xu,

Xiaomei Tang

et al.

Antioxidants, Journal Year: 2020, Volume and Issue: 9(2), P. 161 - 161

Published: Feb. 17, 2020

CYTOCHROME P450s genes are a large gene family in the plant kingdom. Our earlier transcriptome data revealed that P450 of Citrus sinensis (CsCYT75B1) was associated with flavonoid metabolism and highly induced after drought stress. Here, we characterized function CsCYT75B1 tolerance by overexpressing it Arabidopsis thaliana. results demonstrated overexpression significantly enhanced total contents increased antioxidant activity transgenic Arabidopsis. The expression showed several responsible for biosynthesis flavonoids were 2–12 fold lines. After 14 days stress, all lines displayed an to stress along accumulating lower superoxide radicals reactive oxygen species (ROS) than wild type plants. In addition, drought-stressed possessed higher enzymatic activities Moreover, stressed had levels electrolytic leakage These demonstrate sweet orange functions contributes elevating ROS scavenging activities.

Language: Английский

Citations

106

Rice metabolic regulatory network spanning the entire life cycle DOI Creative Commons
Chenkun Yang, Shuangqian Shen, Shen Zhou

et al.

Molecular Plant, Journal Year: 2021, Volume and Issue: 15(2), P. 258 - 275

Published: Oct. 27, 2021

Language: Английский

Citations

96

Mutations in MIR396e and MIR396f increase grain size and modulate shoot architecture in rice DOI Creative Commons
Chunbo Miao, Dong Wang,

Reqing He

et al.

Plant Biotechnology Journal, Journal Year: 2019, Volume and Issue: 18(2), P. 491 - 501

Published: July 23, 2019

Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR396 family in rice found that MIR396e MIR396f two important regulators grain architecture. mir396ef mutations can increase yield by increasing size. In addition, resulted an altered architecture, with lengthened leaves but shortened internodes, especially uppermost internode. Our research suggests promote leaf elongation level a gibberellin (GA) precursor, mevalonic acid, which subsequently promotes GA biosynthesis. However, internode mutants appears to be suppressed via reduced CYP96B4 expression not pathway. This provides candidate gene-editing targets breed elite varieties.

Language: Английский

Citations

91

The IAA- and ABA-responsive transcription factor CgMYB58 upregulates lignin biosynthesis and triggers juice sac granulation in pummelo DOI Creative Commons

Meiyan Shi,

Xiao Liu,

Haipeng Zhang

et al.

Horticulture Research, Journal Year: 2020, Volume and Issue: 7(1)

Published: Sept. 1, 2020

In citrus, lignin overaccumulation in the juice sac results granulation and an unpleasant fruit texture taste. By integrating metabolic phenotyping transcriptomic analyses, we found 702 differentially expressed genes (DEGs), including 24 transcription factors (TFs), to be significantly correlated with content. CgMYB58 was further identified as a critical R2R3 MYB TF involved owing its high transcript levels Huanong Red-fleshed pummelo (HR, Citrus grandis) fruits. Transient expression of led increase content mesocarp, whereas stable overexpression promoted accumulation upregulated 19 biosynthetic genes. Among these genes, CgPAL1, CgPAL2, Cg4CL1, CgC3H were directly modulated by through interaction their promoter regions. Moreover, showed that fruits could affected indole-3-acetic acid (IAA) abscisic (ABA) treatments. HR pummelo, ABA accelerated this granulation, IAA effectively inhibited process. Taken together, provide novel insight into mechanism citrus We also revealed theoretical basis via exogenous application, which repressed target thus alleviating orchards.

Language: Английский

Citations

77

Review of phytochemical and nutritional characteristics and food applications of Citrus L. fruits DOI Creative Commons
Shuxun Liu,

Ying Lou,

Yixian Li

et al.

Frontiers in Nutrition, Journal Year: 2022, Volume and Issue: 9

Published: July 18, 2022

Since the dietary regimen rich in fruits is being widely recognized and encouraged, Citrus L. have been growing popularity worldwide due to their high amounts of health-promoting phytonutrients bioactive compounds, such as flavonoids, phenolic acids, vitamins, carotenoids, pectins, fatty acids. The diverse physicochemical properties multiple utilization citrus food industry are associated with unique chemical compositions. Throughout world, has used for producing various value-added nutritionally enhanced products, including juices, wines, jams, canned citrus, dried citrus. However, current studies regarding phytochemical nutritional characteristics applications scattered. This review systematically summarizes existing bibliography on characteristics, functional nutraceutical benefits, processing, potential A thorough understanding this information may provide scientific guidance better utilizing a fruit benefit extension value chain.

Language: Английский

Citations

59

Citrus reticulata Blanco (the common mandarin) fruit: An updated review of its bioactive, extraction types, food quality, therapeutic merits, and bio-waste valorization practices to maximize its economic value DOI
Mohamed Shorbagi, Nesrin M. Fayek,

Ping Shao

et al.

Food Bioscience, Journal Year: 2022, Volume and Issue: 47, P. 101699 - 101699

Published: April 4, 2022

Language: Английский

Citations

44

Phytochemistry and biological activities of Citrus sinensis and Citrus limon: an update DOI
Sarah Zahr, Rayan Zahr, Rana El Hajj

et al.

Journal of Herbal Medicine, Journal Year: 2023, Volume and Issue: 41, P. 100737 - 100737

Published: Aug. 15, 2023

Language: Английский

Citations

29