Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

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Tachykinins: Neuropeptides That Are Ancient, Diverse, Widespread and Functionally Pleiotropic

Frontiers in Neuroscience, Journal Year: 2019, Volume and Issue: 13

Published: Nov. 20, 2019

Tachykinins (TKs) are ancient neuropeptides present throughout the bilaterians and are, with some exceptions, characterized by a conserved FX1GX2Ramide carboxy terminus among protostomes FXGLMamide in deuterostomes. The best-known TK is vertebrate substance P, which mammals, together other TKs, has been implicated health disease important roles pain, inflammation, cancer, depressive disorder, immune system, gut function, hematopoiesis, sensory processing, hormone regulation. invertebrate TKs also known to have multiple functions central nervous system intestine these investigated more detail fly Drosophila arthropods. Here, we review protostome deuterostome organization evolution of precursors, peptides their receptors, as well functions, appear be partly across Bilateria. We outline distribution brains representative organisms. In Drosophila, recent studies revealed early olfactory neuromodulation circuits controlling locomotion food search, nociception, aggression, metabolic stress, release. signaling regulates lipid metabolism intestine. crustaceans, an neuromodulator rhythm-generating motor stomatogastric presynaptic modulator photoreceptor cells. Several additional functional can inferred from various brain circuits. addition, there few interesting cases where injected into prey animals vasodilators salivary glands or paralyzing agents venom glands. cases, produced predator sequences mimicking TKs. Lastly, TK-signaling appears duplicated Panarthropoda (comprising arthropods, onychophores, tardigrades) give rise novel type peptides, natalisins, distinct receptor. natalisins general, it that widely distributed act at short range neuromodulators cotransmitters.

Language: Английский

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Evolutionary conserved peptide and glycoprotein hormone-like neuroendocrine systems in C. elegans

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 584, P. 112162 - 112162

Published: Jan. 28, 2024

Peptides and protein hormones form the largest group of secreted signals that mediate intercellular communication are central regulators physiology behavior in all animals. Phylogenetic analyses biochemical identifications peptide-receptor systems reveal a broad evolutionary conservation these signaling at molecular level. Substantial progress has been made recent years on characterizing physiological putative ancestral roles many peptide through comparative studies invertebrate models. Several peptides not only molecularly conserved but also have across animal phyla. Here, we focus functional insights gained nematode Caenorhabditis elegans that, with its compact well-described nervous system, provides powerful model to dissect neuroendocrine networks involved control behavior. We summarize discoveries knowledge functions hormone C. elegans.

Language: Английский

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Ancient role of vasopressin/oxytocin-type neuropeptides as regulators of feeding revealed in an echinoderm

BMC Biology, Journal Year: 2019, Volume and Issue: 17(1)

Published: July 30, 2019

Vasopressin/oxytocin (VP/OT)-type neuropeptides are well known for their roles as regulators of diuresis, reproductive physiology and social behaviour. However, our knowledge functions is largely based on findings from studies vertebrates selected protostomian invertebrates. Little about the VP/OT-type in deuterostomian invertebrates, which more closely related to than protostomes.

Language: Английский

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Ancient role of sulfakinin/cholecystokinin-type signalling in inhibitory regulation of feeding processes revealed in an echinoderm

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Sept. 7, 2021

Sulfakinin (SK)/cholecystokinin (CCK)-type neuropeptides regulate feeding and digestion in protostomes (e.g. insects) chordates. Here, we characterised SK/CCK-type signalling for the first time a non-chordate deuterostome – starfish Asterias rubens (phylum Echinodermata). In this species, two (ArSK/CCK1, ArSK/CCK2) derived from precursor protein ArSK/CCKP act as ligands an receptor (ArSK/CCKR) these peptides/proteins are expressed nervous system, digestive tube feet, body wall. Furthermore, ArSK/CCK1 ArSK/CCK2 cause dose-dependent contraction of cardiac stomach, foot, apical muscle preparations vitro, injection vivo triggers stomach retraction inhibition onset A. . Thus, evolutionarily ancient role inhibitory regulators feeding-related processes Bilateria has been conserved unusual unique context extra-oral behaviour pentaradial plan echinoderm.

Language: Английский

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Neuropeptide precursors and neuropeptides in the sea cucumber Apostichopus japonicus: a genomic, transcriptomic and proteomic analysis

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: June 20, 2019

The sea cucumber Apostichopus japonicus is a foodstuff with very high economic value in China, Japan and other countries south-east Asia. It at the heart of multibillion-dollar industry to meet demand for this product, aquaculture methods facilities have been established. However, there are challenges associated optimization reproduction, feeding growth non-natural environments. Therefore, we need learn more about biology A. japonicus, including processes such as aestivation, evisceration, regeneration albinism. One major classes molecules that regulate physiology behaviour animals neuropeptides, few bioactive peptides already identified japonicus. To facilitate comprehensive investigations neuropeptide function here analysed genomic transcriptomic sequence data proteomic identify precursors neuropeptides species. We 44 transcripts encoding or putative precursors, some instances derived from these were confirmed by mass spectrometry. Furthermore, analysis enabled identification location precursor genes on scaffolds linkage groups (chromosomes) determination gene structure. Many contain homologs bilaterian animals. Precursors thus far only echinoderms identified, L- F-type SALMFamides, AN others. several act modulators neuromuscular activity also identified. discovery large repertoire provides basis experimental studies investigate physiological roles signaling systems Looking ahead, may effects could be harnessed enable improvements economically important

Language: Английский

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Echinoderms provide missing link in the evolution of PrRP/sNPF-type neuropeptide signalling

eLife, Journal Year: 2020, Volume and Issue: 9

Published: June 24, 2020

Neuropeptide signalling systems comprising peptide ligands and cognate receptors are evolutionarily ancient regulators of physiology behaviour. However, there challenges associated with determination orthology between neuropeptides in different taxa. Orthologs vertebrate neuropeptide-Y (NPY) known as neuropeptide-F (NPF) have been identified protostome invertebrates, whilst prolactin-releasing (PrRP) short (sNPF) paralogs NPY/NPF vertebrates protostomes, respectively. Here we investigated the occurrence NPY/NPF/PrRP/sNPF-related a deuterostome invertebrate phylum - Echinodermata. Analysis transcriptome/genome sequence data revealed loss NPY/NPF-type signalling, but orthologs PrRP-type sNPF/PrRP-type were echinoderms. Furthermore, experimental studies that neuropeptide pQDRSKAMQAERTGQLRRLNPRF-NH2 is potent ligand for receptor starfish Asterias rubens. Our findings indicate sNPF-type orthologous originated paralog Urbilateria.

Language: Английский

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Somatostatin-type and allatostatin-C–type neuropeptides are paralogous and have opposing myoregulatory roles in an echinoderm

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(7)

Published: Feb. 10, 2022

Somatostatin (SS) and allatostatin-C (ASTC) are inhibitory neuropeptides in chordates protostomes, respectively, which hitherto were identified as orthologs. However, echinoderms have two SS/ASTC-type (SS1 SS2), here, our analysis of sequence data indicates that SS1 is an ortholog ASTC SS2 SS. The occurrence both SS-type ASTC-type provides a unique context to compare their physiological roles. Investigation the expression actions neuropeptide ArSS1 starfish Asterias rubens revealed it causes muscle contraction (myoexcitation), contrasting with myoinhibitory effects ArSS2. Our findings suggest paralogous originated by gene duplication common ancestor Bilateria, only one type being retained protostomes but types echinoderms. Loss may been due functional redundancy regulators processes. Conversely, retention be consequence evolution myoexcitatory role for mediated yet unknown signaling mechanisms.

Language: Английский

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Biochemical, Anatomical, and Pharmacological Characterization of Calcitonin-Type Neuropeptides in Starfish: Discovery of an Ancient Role as Muscle Relaxants

Frontiers in Neuroscience, Journal Year: 2018, Volume and Issue: 12

Published: June 8, 2018

Calcitonin (CT) is a peptide hormone released by the thyroid gland that regulates blood Ca2+ levels in mammals. The CT gene alternatively spliced, with one transcript encoding and another CT-like neuropeptide calcitonin-gene related (α-CGRP), which powerful vasodilator. Other CT-related peptides vertebrates include adrenomedullin, amylin intermedin, also act as smooth muscle relaxants. evolutionary origin of CT-type has been traced to bilaterian common ancestor protostomes deuterostomes (DH31) identified diuretic some insect species. However, little known about physiological roles other invertebrates. Here we characterized deuterostomian invertebrate – starfish Asterias rubens (Phylum Echinodermata). A precursor cDNA (ArCTP) was sequenced predicted structure (ArCT) derived from ArCTP confirmed using mass spectrometry. distribution mRNA ArCT investigated situ hybridization immunohistochemistry, respectively, revealing stained cells/processes nervous system, digestive system muscular organs, including apical tube feet. Investigation effects synthetic on vitro preparations feet revealed it acts relaxant, causing dose-dependent reversal acetylcholine-induced contraction. Furthermore, relaxant present whole-animal extracts species, Patiria pectinifera, an ortholog named PpCT. Consistent expression pattern A. rubens, RT-qPCR P. pectinifera PpCT more abundant radial nerve cords than tissues/organs analyzed. In conclusion, our findings indicate action relaxants may reflect evolutionarily ancient role neuropeptides can be back deuterostomes.

Language: Английский

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Molecular and functional characterization of somatostatin-type signalling in a deuterostome invertebrate

Open Biology, Journal Year: 2020, Volume and Issue: 10(9)

Published: Sept. 1, 2020

Somatostatin (SS) and allatostatin-C (ASTC) are structurally evolutionarily related neuropeptides that act as inhibitory regulators of physiological processes in mammals insects, respectively. Here, we report the first molecular functional characterization SS/ASTC-type signalling a deuterostome invertebrate—the starfish Asterias rubens (phylum Echinodermata). Two precursors were identified A. (ArSSP1 ArSSP2) structures derived from these proteins (ArSS1 ArSS2) analysed using mass spectrometry. Pharmacological three cloned receptors (ArSSR1–3) revealed ArSS2, but not ArSS1, acts ligand for all receptors. Analysis ArSS2 expression mRNA situ hybridization immunohistochemistry stained cells/fibres central nervous system, digestive system (e.g. cardiac stomach) body wall its appendages tube feet). Furthermore, vitro pharmacological tests causes dose-dependent relaxation foot stomach preparations, while injection vivo partial eversion stomach. Our findings provide new insights into evolution animal kingdom reveal an ancient role SS-type muscle contractility.

Language: Английский

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