Stem Cell Research,
Journal Year:
2022,
Volume and Issue:
63, P. 102851 - 102851
Published: July 7, 2022
Type
2
diabetes
mellitus
(T2DM)
is
a
major
caused
by
insulin
resistance
with
relative
deficiency
in
secretion.
Statistically,
T2DM
accounts
for
90%
of
cases
worldwide.
We
report
the
patient-specific
human
induced
pluripotent
stem
cell
line
(iPSC)
CSUASOi010-A
using
Peripheral
blood
mononuclear
cells
(PBMCs)
62-year-old
female
from
(T2DM).
Patient
blood-derived
were
reprogrammed
Sendai
virus.
Frontiers in Oncology,
Journal Year:
2022,
Volume and Issue:
12
Published: Feb. 25, 2022
Breast
cancer
(BRCA)
has
become
the
most
frequently
appearing,
lethal,
and
aggressive
with
increasing
morbidity
mortality.
Previously,
it
was
discovered
that
HAUS5
protein
is
involved
in
centrosome
integrity,
spindle
assembly,
completion
of
cytoplasmic
division
process
during
mitosis.
By
encouraging
chromosome
misdivision
aneuploidy,
potential
to
cause
cancer.
The
significance
BRCA
relationship
between
its
expression
clinical
outcomes
or
immune
infiltration
remains
unclear.Pan-cancer
analyzed
by
TIMER2
web
differential
discovered.
prognostic
value
for
evaluated
KM
plotter
confirmed
Gene
Expression
Omnibus
(GEO)
dataset.
Following
that,
we
looked
at
high
low
groups
breast
indications.
Signaling
pathways
linked
were
using
Set
Enrichment
Analysis
(GSEA).
relative
cell
infiltrations
each
sample
assessed
CIBERSORT
algorithm
ESTIMATE
method.
We
Tumor
Mutation
Burden
(TMB)
two
sets
samples
expression,
as
well
differences
gene
mutations
groups.
proliferation
changes
cells
after
knockdown
fluorescence
counting
colony
formation
assay.HAUS5
strongly
expressed
malignancies,
distinct
associations
exist
prognosis
patients.
Upregulated
associated
poor
clinicopathological
characteristics
such
tumor
T
stage,
ER,
PR,
HER2
status.
mitotic
prometaphase,
primary
immunodeficiency,
DNA
replication,
cycle
related
signaling
all
enriched
presence
elevated
according
GSEA
analysis.
microenvironment's
core
gene,
HAUS5,
shown
be
invading
subtypes
stemness.
TMB
HAUS5-low
group
significantly
higher
than
group.
mutation
frequency
15
genes
substantially
different
compared
cells'
capacity
proliferate
decreased
when
knocked
down.These
findings
show
a
positive
regulator
progression
contributes
proliferation.
As
result,
might
novel
indicator
therapeutic
target
Cells,
Journal Year:
2022,
Volume and Issue:
11(17), P. 2719 - 2719
Published: Aug. 31, 2022
The
emergence
of
drug
resistance
in
cancer
poses
the
greatest
hurdle
for
successful
therapeutic
results
and
is
associated
with
most
deaths.
In
triple
negative
breast
(TNBC),
due
to
lack
specific
targets,
systemic
chemotherapy
at
forefront
treatments,
but
it
only
benefits
a
fraction
patients
because
development
resistance.
Cancer
cells
may
possess
an
innate
chemotherapeutic
agents
or
develop
new
mechanisms
acquired
after
long-term
exposure.
Such
involve
interplay
between
genetic,
epigenetic
metabolic
alterations
that
enable
evade
therapy.
this
work,
we
generated
characterized
chemoresistant
TNBC
cell
line
be
used
investigation
drive
paclitaxel.
Transcriptomic
analysis
highlighted
important
role
metabolic-associated
pathways
resistant
cells,
prompting
us
employ
1H-NMR
explore
metabolome
lipidome
these
cells.
We
identified
described
herein
numerous
metabolites
lipids
were
significantly
altered
Integrated
our
omics
data
revealed
MSMO1,
intermediate
enzyme
cholesterol
biosynthesis,
as
novel
mediator
chemoresistance
TNBC.
Overall,
provide
critical
insight
into
adaptations
accompany
pinpoint
potential
targets.
Frontiers in Oncology,
Journal Year:
2022,
Volume and Issue:
12
Published: June 14, 2022
The
majority
of
breast
cancers
are
estrogen
receptor
(ER)+
and
agents
targeting
the
ER
signaling
pathway
have
markedly
increased
survival
for
women
with
cancer
decades.
However,
therapeutic
resistance
eventually
emerges,
especially
in
metastatic
setting.
In
past
decade
disrupted
epigenetic
regulatory
processes
emerged
as
major
contributors
to
carcinogenesis
many
types.
Aberrations
chromatin
modifiers
transcription
factors
also
been
recognized
mediators
development
outcome,
new
epigenetic-based
therapies
combination
targeted
proposed.
Here
we
will
discuss
recent
progress
our
understanding
chromatin-based
mechanisms
tumorigenesis,
how
these
affect
response
standard
care
treatment,
strategies
towards
intervention
overcome
resistance.
Cancers,
Journal Year:
2022,
Volume and Issue:
14(13), P. 3057 - 3057
Published: June 22, 2022
Triple-negative
breast
cancer
(TNBC)
accounts
for
approximately
20%
of
all
carcinomas
and
has
the
worst
prognosis
subtypes
due
to
lack
an
effective
target.
Therefore,
understanding
molecular
mechanism
underpinning
TNBC
progression
could
explore
a
new
target
therapy.
While
Notch
pathway
is
critical
in
development
process,
its
dysregulation
leads
initiation.
Previously,
we
found
that
manic
fringe
(MFNG)
activates
signaling
induces
progression.
However,
underlying
MFNG
upstream
remains
unknown.
In
this
study,
regulatory
mechanisms
TNBC.
We
show
increased
expression
associated
with
poor
clinical
significantly
promotes
cell
growth
migration,
as
well
activation.
The
mechanistic
studies
reveal
direct
GATA3
miR205-5p
demonstrate
overexpression
attenuate
oncogenic
effects,
while
knockdown
mimics
phenotype
promote
Moreover,
illustrate
required
activation
inhibit
transcription
by
binding
3'
UTR
region
mRNA,
which
forms
GATA3/miR205-5p/MFNG
feed-forward
loop.
Additionally,
our
vivo
data
mimic
combined
polyetherimide-black
phosphorus
(PEI-BP)
nanoparticle
remarkably
inhibits
TNBC-derived
tumors
expression.
Collectively,
study
uncovers
novel
loop
be
potential
therapeutic
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2021,
Volume and Issue:
unknown
Published: May 12, 2021
Abstract
Micro-environmental
and
molecular
factors
mediating
the
progression
of
Breast
Ductal
Carcinoma
In
Situ
(DCIS)
are
not
well
understood,
impeding
development
prevention
strategies
safe
testing
treatment
de-escalation.
We
addressed
methodological
barriers
characterized
mutational,
transcriptional,
histological
microenvironmental
landscape
across
85
multiple
micro-dissected
regions
from
39
cases.
Most
somatic
alterations,
including
whole
genome
duplications,
were
clonal,
but
genetic
divergence
increased
with
physical
distance.
Phenotypic
subtype
heterogeneity
frequently
associated
underlying
low-risk
features
preceded
those
high-risk
according
to
inferred
phylogeny.
B-
T-lymphocytes
spatial
analysis
identified
3
immune
states,
an
epithelial
excluded
state
located
preferentially
at
DCIS
regions,
by
escape,
independently
subtypes.
Such
breast
pre-cancer
atlas
uniquely
integrated
observations
will
help
scope
future
expansion
studies
build
finer
models
outcomes
risk.
Stem Cell Research,
Journal Year:
2022,
Volume and Issue:
63, P. 102851 - 102851
Published: July 7, 2022
Type
2
diabetes
mellitus
(T2DM)
is
a
major
caused
by
insulin
resistance
with
relative
deficiency
in
secretion.
Statistically,
T2DM
accounts
for
90%
of
cases
worldwide.
We
report
the
patient-specific
human
induced
pluripotent
stem
cell
line
(iPSC)
CSUASOi010-A
using
Peripheral
blood
mononuclear
cells
(PBMCs)
62-year-old
female
from
(T2DM).
Patient
blood-derived
were
reprogrammed
Sendai
virus.
