Dynamically expressed single ELAV/Hu orthologue elavl2 of bees is required for learning and memory

Communications Biology, Journal Year: 2021, Volume and Issue: 4(1)

Published: Oct. 28, 2021

Abstract Changes in gene expression are a hallmark of learning and memory consolidation. Little is known about how alternative mRNA processing, particularly abundant neuron-specific genes, contributes to these processes. Prototype RNA binding proteins the neuronally expressed ELAV/Hu family candidates for roles memory, but their capacity cross-regulate take over each other’s functions complicate substantiation such links. Honey bees Apis mellifera have only one elav/Hu elavl2 , that has functionally diversified by increasing splicing including an evolutionary conserved microexon. RNAi knockdown demonstrates ELAVL2 required bees. dynamically with altered subcellular localization mushroom bodies, not other brain regions. Expression change during consolidation illustrating processing program as part local response underlying

Language: Английский

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Unravelling nicotinic receptor and ligand features underlying neonicotinoid knockdown actions on the malaria vector mosquito Anopheles gambiae

Open Biology, Journal Year: 2024, Volume and Issue: 14(7)

Published: July 1, 2024

With the spread of resistance to long-established insecticides targeting Anopheles malaria vectors, understanding actions compounds newly identified for vector control is essential. new commercial vector-control products containing neonicotinoids under development, we investigate 6 (imidacloprid, thiacloprid, clothianidin, dinotefuran, nitenpyram and acetamiprid) on 13 gambiae nicotinic acetylcholine receptor (nAChR) subtypes produced by expression combinations Ag α 1, 2, 3, 8 β 1 subunits in Xenopus laevis oocytes, Drosophila melanogaster orthologues which have previously shown be important neonicotinoid actions. The presence 2 subunit reduces affinity mosquito nAChRs, whereas 3 increases it. Crystal structures binding protein (AChBP), an established surrogate ligand-binding domain, with dinotefuran bound, shows a unique target site interaction through hydrogen bond formation CH-N at tetrahydrofuran ring. This interest as also trial toxic element baited traps. Multiple regression analyses show correlation between efficacy 1/Ag 2/Ag 8/Ag nAChR, their hydrophobicity rate knockdown adult female An. , providing insights into features control.

Language: Английский

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DYRK1a Inhibitor Mediated Rescue of Drosophila Models of Alzheimer’s Disease-Down Syndrome Phenotypes

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: July 19, 2022

Alzheimer's disease (AD) is the most common neurodegenerative which becoming increasingly prevalent due to ageing populations resulting in huge social, economic, and health costs community. Despite pathological processing of genes such as Amyloid Precursor Protein (APP) into Amyloid-β Microtubule Associated Tau (MAPT) gene, hyperphosphorylated tangles being known for decades, there remains no treatments halt progression. One population with increased risk AD are people Down syndrome (DS), who have a 90% lifetime incidence AD, trisomy human chromosome 21 (HSA21) three copies APP other AD-associated genes, DYRK1A (Dual specificity tyrosine-phosphorylation-regulated kinase 1A) overexpression. This suggests that blocking might therapeutic potential. However, it still not clear what extent overexpression by itself leads AD-like phenotypes how these compare mediated pathology. Likewise, effective antagonist may be at preventing or improving any Tau, DYRK1a phenotype. To address outstanding questions, we characterised Drosophila models targeted fly orthologue DYRK1A, called minibrain (mnb). We found caused degeneration photoreceptor neurons, shortened lifespan, well causing loss locomotor performance, sleep, memory. Treatment experimental inhibitor PST-001 decreased phosphorylation [at serine (S) 262]. reduced mnb lengthening lifespan locomotion, sleep memory expression DS genes. demonstrated effectiveness potential new targeting

Language: Английский

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Neonicotinoid insecticide imidacloprid induces chemosensory deficits in a nontarget parasitoid wasp

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 907, P. 168089 - 168089

Published: Oct. 23, 2023

Language: Английский

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Systematic analysis of circadian disrupting substances with a high-throughput zebrafish circadian behavior screening approach

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 904, P. 167037 - 167037

Published: Sept. 12, 2023

Language: Английский

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