Brain Proteome and Behavioural Analysis in Wild Type, BDNF+/− and BDNF−/− Adult Zebrafish (Danio rerio) Exposed to Two Different Temperatures DOI Open Access
Elisa Maffioli,

Elisa Angiulli,

Simona Nonnis

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(10), P. 5606 - 5606

Published: May 17, 2022

Experimental evidence suggests that environmental stress conditions can alter the expression of BDNF and this neurotrophin influences behavioural responses in mammalian models. It has been recently demonstrated exposure to 34 °C for 21 days alters brain proteome behaviour zebrafish. The aim work was investigate role nervous system adult zebrafish under control heat treatment conditions. For purpose, from three different genotypes (wild type, heterozygous BDNF+/- knock out BDNF-/-) were kept at 26 or then euthanized molecular analyses subjected tests (Y-maze test, novel tank light dark social preference mirror biting test) assessing aspects such as boldness, anxiety, preference, aggressive behaviour, interest environment exploration. qRT-PCR analysis showed reduction gene its receptors after wild type Moreover, proteomic genotype- temperature-dependent effects on responding. Overall, absent KO (1) by reducing proteins involved synapse functioning neurotransmitter-mediated transduction; (2) which be interpreted bolder less anxious (3) cellular response thermal treatment.

Language: Английский

Water Quality Impact on Fish Behavior: A Review From an Aquaculture Perspective DOI
Kaisheng Zhang, Zhangying Ye, Ming Qi

et al.

Reviews in Aquaculture, Journal Year: 2024, Volume and Issue: 17(1)

Published: Nov. 6, 2024

ABSTRACT Changes in water quality significantly shape fish behavior, a crucial index reflecting the growth and welfare status of fish. Given centrality this relationship to aquaculture practices, comprehensive understanding how dynamics influence behavior is imperative. While there have been some summaries effects parameters on physiology growth, few reviews their reported yet. This article several which are great concern from multiple facets actual production, including physical (water temperature turbidity), chemical (dissolved oxygen, salinity, pH, inorganic nitrogen), pollutants (microplastics crude oil), gained increasing attention researchers practitioners over past decades. Variations these can exert profound physiology, metabolism, internal tissues organs, sensory perception, influences behaviors such as swimming, schooling, feeding, predation, anti‐predation, aggression, courtship, well adaptive stress‐related exploration, avoidance response, anxiety‐like behavior. By synthesizing behavioral changes caused by specific parameters, review aims provide strong support for further quality‐related research, thereby fostering environments conducive both productivity.

Language: Английский

Citations

25
Improving zebrafish laboratory welfare and scientific research through understanding their natural history DOI Creative Commons
Carole J. Lee, Gregory C. Paull, Charles R. Tyler

et al.

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2022, Volume and Issue: 97(3), P. 1038 - 1056

Published: Jan. 4, 2022

ABSTRACT Globally, millions of zebrafish ( Danio rerio ) are used for scientific laboratory experiments which researchers have a duty care, with legal obligations to consider their welfare. Considering the growing use as vertebrate model addressing diverse range questions, optimising conditions is major importance both welfare and improving research. However, most guidelines care breeding research concerned primarily maximising production minimising costs pay little attention effects on environments in fish maintained, or how those affect Here we review physical social kept, identifying drawing factors likely experimental science. We also identify fundamental lack knowledge interact many biotic abiotic features natural environment support ways optimise health well‐being laboratory, turn quality data produced. advocate that under maintained need become more integral part understand fully they influence outcome interpretations generated.

Language: Английский

Citations

40
Temperature effects on development and lifelong behavior in zebrafish DOI
Augusto Monteiro de Souza, Francisco Carlos da Silva,

Éntony David Dantas

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 973, P. 179172 - 179172

Published: March 20, 2025

Language: Английский

Citations

1
Enrofloxacin exposure induces anxiety-like behavioral responses in zebrafish by affecting the microbiota-gut-brain axis DOI

Dandan Tian,

Wei Shi, Yihan Yu

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 858, P. 160094 - 160094

Published: Nov. 11, 2022

Language: Английский

Citations

33
Heat stress affects swimming performance and induces biochemical, structural, and transcriptional changes in the heart of Gymnocypris eckloni DOI Creative Commons
Junting Li, Yuting Duan, Weiqiang Kong

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 35, P. 101998 - 101998

Published: Feb. 29, 2024

Global warming has heightened the severity and frequency of extreme high-temperature events, presenting challenges to fish survival negatively affecting production fishery. Gymnocypris eckloni, native Tibetan Plateau, is widely cultured in southwest China. Its sensitivity heat makes it a suitable model for studying impact high temperatures on fish. The heart vital organ survival, while relatively understudied concerning hearts. In this study, G. eckloni was subjected seven-day exposure 27 °C followed by transfer 18 recovery period. Behavioral tests revealed significant increase locomotor speed, distance, respiratory rate at °C. Serum indices exhibited significantly elevated stress indicators (cortisol) kinases associated with damage (lactate dehydrogenase, lactate dehydrogenase isoenzyme 1, creatine kinase, kinase isoenzyme, aspartate transaminase) °C, histological analyses have confirmed occurrence temperatures. After 7 days recovery, most these showed decrease compared group, no differences observed control group. Transcriptomic widespread involvement ferroptosis, P53 signaling pathway, oxidative phosphorylation, myocardial contraction, DNA repair pathway response Our study offers systematic exploration effects providing valuable insights cultivation high-altitude

Language: Английский

Citations

7
Comparative transcriptomic and epigenomic analyses to identify the cold resistance-associated genes and disclose the regulatory mechanisms in tilapias DOI

Guili Song,

Ran Liu,

Shaoxiong Chen

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 587, P. 740858 - 740858

Published: March 21, 2024

Language: Английский

Citations

7
Quantitative proteomics insights into gel properties changes of myofibrillar protein from Procambarus clarkii under cold stress DOI
Xuehong Li, Shugang Li,

Gangpeng Shi

et al.

Food Chemistry, Journal Year: 2021, Volume and Issue: 372, P. 130935 - 130935

Published: Aug. 23, 2021

Language: Английский

Citations

24
Temperature- and chemical-induced neurotoxicity in zebrafish DOI Creative Commons
Mattia Toni, Chiara Arena,

Carla Cioni

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 3, 2023

Throughout their lives, humans encounter a plethora of substances capable inducing neurotoxic effects, including drugs, heavy metals and pesticides. Neurotoxicity manifests when exposure to these chemicals disrupts the normal functioning nervous system, some agents have been linked neurodegenerative pathologies such as Parkinson's Alzheimer's disease. The growing concern surrounding impacts both naturally occurring man-made toxic necessitates identification animal models for rapid testing across wide spectrum concentrations, utilization tools detecting system alterations spanning from molecular level up behavioural one. Zebrafish (Danio rerio) is gaining prominence in field neuroscience due its versatility. possibility analysing all developmental stages (embryo, larva adult), applying most common "omics" approaches (transcriptomics, proteomics, lipidomics, etc.) conducting range tests makes zebrafish an excellent model neurotoxicity studies. This review delves into main experimental adopted markers analysed studies zebrafish, showing that phenomena can be triggered not only by chemical but also fluctuations temperature. findings presented here serve valuable resource study define new scenarios ecotoxicology suggesting temperature synergistically compound effects substances, intensifying detrimental impact on fish populations.

Language: Английский

Citations

10
Olanzapine exposure disordered lipid metabolism, gut microbiota and behavior in zebrafish (Danio rerio) DOI
Yawei Wang,

Fu You,

Zhenyi Huang

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2025, Volume and Issue: 289, P. 110120 - 110120

Published: Jan. 7, 2025

Language: Английский

Citations

0
Genetics of Physiological Variation Within and Between Larval Wild-Type AB and Backcrossed NHGRI-1 Zebrafish (Danio rerio) DOI Creative Commons
Gil Martínez-Bautista,

Moira Ryann Cartee,

Dyuksha Kunder

et al.

Fishes, Journal Year: 2025, Volume and Issue: 10(2), P. 59 - 59

Published: Jan. 31, 2025

Changes in the environment promote variations fish physiological responses. Genetic variation also plays a role variation. To explore of genetics variation, we assessed cardiac function (heart rate, stroke volume, and output), oxygen consumption, yolk conversion efficiency, cost development embryonic larval AB wild-type NHGRI-1 zebrafish (low heterozygosity line backcrossed from wild-type) exposed to different temperature regimes. Fish were fertilization 7 days post-fertilization (dpf) control conditions (28 °C, 21% O2) or low (23 O2), high (33 moderate hypoxia 13% severe 10% O2). We hypothesized that (1) variables will respond similarly both lines (2) data variability be lower than zebrafish. Cardiac decreased at Oxygen consumption was increased by higher fish. Yolk efficiency Cost mainly hypoxia-treated Supporting our hypothesis contributes showed significantly coefficients 84% endpoints. conclude there is strong genetic component fishes are useful models for reducing ‘noise’ backgrounds research fish, which may aid interpretation experimental results facilitate reproducibility.

Language: Английский

Citations

0