bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 14, 2023
Maximizing
the
life-long
reproductive
output
would
lead
to
prediction
that
short-lived
and
fast
aging
species
undergo
no
-
if
any
senescence.
Turquoise
killifish
(Nothobranchius
furzeri)
are
naturally
teleosts,
extensive
somatic
aging,
characterized
by
molecular,
cellular,
organ
dysfunction
following
onset
of
sexual
maturation.
Here,
we
tested
whether
turquoise
males
maximize
reproduction
display
minimal
any,
We
analysed
age-related
changes
in
sperm
traits,
proportion
fertilized
eggs,
as
well
embryo
survival.
Contrary
expectation
found
consisting
age-dependent
decline
quality,
decreased
eggs
lower
Our
results
indicate
male
do
not
trade-off
soma
with
sustained
fitness.
Instead,
appear
occur
simultaneously.
findings
question
generalized
vs.
senescence
models
highlight
importance
integrating
species-specific
ecological
demographic
constraints
explain
observed
life
history
traits.
BMC Ecology and Evolution,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Jan. 6, 2025
Abstract
Maximizing
the
life-long
reproductive
output
would
lead
to
prediction
that
short-lived
and
fast
aging
species
undergo
no
–
if
any
senescence.
Turquoise
killifish
(
Nothobranchius
furzeri
)
are
naturally
teleosts,
extensive
somatic
aging,
characterized
by
molecular,
cellular,
organ
dysfunction
following
onset
of
sexual
maturation.
Here,
we
tested
whether
male
turquoise
maximize
reproduction
display
minimal
any,
We
analysed
age-related
changes
in
sperm
traits,
proportion
fertilized
eggs,
as
well
embryo
survival.
Contrary
expectation
found
males
consisting
age-dependent
decline
quality,
decreased
eggs
lower
Our
results
indicate
do
not
trade-off
soma
with
sustained
fitness.
Instead,
appear
occur
simultaneously.
findings
question
generalized
vs.
senescence
models
highlight
importance
integrating
species-specific
ecological
demographic
constraints
explain
observed
life
history
traits.
Zebrafish
as
a
model
animal
widely
used
in
toxicological
studies
is
highly
sensitive
to
changes
various
environmental
factors.
Many
factors
including
photoperiod,
temperature,
oxygen,
etc.,
dangerous
compounds
such
heavy
metal
Cu,
Cd,
Hg,
Pb,
and
estrogen
BPA,
for
instance,
can
cause
reproductive
toxicity
zebrafish.
The
potential
damage
effects
caused
by
the
combined
contaminants
include
abnormal
gonad
development,
histopathological
changes,
reduced
germ
cell
number
quality,
motility
of
spermatozoa,
altered
follicular
size,
egg
production,
steroid
hormone
biosynthesis,
unbalanced
levels
gene
expression,
fertilization
hatching
success,
larval
juvenile
viability,
etc.
This
chapter
describes
parameters
that
characterize
function
zebrafish,
well
corresponding
detection
methods.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(6), P. 2457 - 2457
Published: March 10, 2025
Sexual
rest
(SR)
in
bulls
leads
to
the
accumulation
of
senescent
spermatozoa
extragonadal
reserves,
potentially
affecting
semen
quality
and
reproductive
efficiency.
Therefore,
this
study
aimed
investigate
impact
SR
on
seminal
plasma
proteome
oxidative
status
Nellore
bulls.
Six
adult
were
subjected
195
days
sequential
collections
using
electroejaculation
method.
The
ejaculates
analyzed
assess
sperm
quality.
Seminal
from
first
last
was
evaluated
for
proteomic
profile
LC-MS.
results
revealed
significant
improvements
motility,
vigor,
antioxidant
enzyme
activity
(superoxide
dismutase
catalase)
ejaculate
compared
first.
Conversely,
higher
levels
markers,
such
as
malondialdehyde
carbonyl
proteins,
observed
ejaculate.
Proteomic
analysis
identified
156
with
28
differentially
abundant
between
ejaculates.
showed
a
abundance
proteins
linked
acrosomal
exocytosis
energy
metabolism,
while
associated
motility
immune
modulation
elevated
These
findings
suggest
that
induces
stress
alterations
plasma,
negatively
quality,
emphasizing
need
strategic
management
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 16, 2025
Abstract
In
many
animals,
sperm
are
stored
for
extended
periods
either
in
the
reproductive
tracts
of
males
before
ejaculation,
or
females
after
copulation.
Sperm
storage
reduces
risk
limitation
both
sexes
and
avoids
costs
female
re-mating.
However,
can
lead
to
post-meiotic
senescence,
i.e.
within-sperm-age-dependent
deterioration,
potentially
impacting
conceived
offspring
lowering
male
fitness.
Yet,
extent
magnitude
such
deterioration
variables
modulating
it
during
not
well
understood.
Using
a
meta-analysis
across
humans
(115
studies)
non-human
animals
(56
studies
from
30
species),
we
investigate
how
in-vivo
affects
quality,
fertilisation
success,
quality.
humans,
leads
greater
oxidative
stress
DNA
damage,
viability
motility.
other
performance
embryo
quality
decline.
We
identify
duration
storage,
design
used
sampling
individuals,
sex
individual
storing
as
important
moderators
effects
storage.
These
findings
have
key
biomedical
implications,
including
optimising
timing
ejaculation
fertility
clinics
captive
breeding
programs.
Overall,
our
results
reveal
mechanisms
that
cause
fitness
consequences
provide
evolutionary
insights
into
sex-specific
adaptations
mitigate
detrimental
Biology Letters,
Journal Year:
2025,
Volume and Issue:
21(4)
Published: April 1, 2025
Inbreeding
can
decrease
male
fertility,
despite
sperm
cells
being
haploid.
However,
to
date,
the
role
of
post-meiotic
senescence
within
female
in
reducing
fertilization
probability
from
inbred
males
is
unknown.
In
this
study,
we
experimentally
created
and
outbred
Japanese
quail
(
Coturnix
japonica
)
let
them
copulate
once
with
a
female.
We
subsequently
monitored
fertility
their
eggs
until
reached
zero,
after
11
days.
Fertilization
was
lower
for
males,
but
decline
over
time
did
not
differ
that
males.
Independent
inbreeding,
accelerated
time.
Hence,
provide
evidence
functionality
occurring
female,
no
inbreeding
effects
on
decline.
Communications Biology,
Journal Year:
2023,
Volume and Issue:
6(1)
Published: March 22, 2023
Abstract
Sperm
competition
is
a
powerful
force
driving
the
evolution
of
ejaculate
and
sperm
traits.
However,
outcome
depends
on
many
traits
that
extend
beyond
quality.
Here,
we
study
male
North
African
houbara
bustards
(
Chlamydotis
undulata
)
competing
for
egg
fertilization,
after
artificial
insemination,
with
aim
to
rank
importance
14
parameters
as
drivers
siring
success.
Using
machine
learning
approach,
show
independent
quality
(i.e.,
insemination
order,
delay
between
laying)
are
most
important
predictors
Traits
describing
intrinsic
number
in
ejaculate,
mass
motility
index)
also
positively
associated
success,
but
their
contribution
explaining
much
lower
than
order.
Overall,
this
analysis
shows
males
mating
at
last
position
sequence
have
best
chance
win
fertilization.
This
raises
question
female
behavior
determinant
Biology Letters,
Journal Year:
2023,
Volume and Issue:
19(6)
Published: June 1, 2023
Mounting
evidence
shows
that
the
female
reproductive
fluid
(FRF)
can
differently
affect
sperm
performance
of
different
males
by
biasing
paternity
share
among
competing
males.
Here,
we
tested
for
first
time
potential
‘within-ejaculate
cryptic
choice’
mediated
FRF
in
zebrafish
(
Danio
rerio
).
Using
a
recently
developed
selection
chamber,
separated
and
collected
FRF-selected
from
non-selected
to
compare
two
subpopulations
terms
number,
viability,
DNA
integrity
fertilizing
ability.
We
showed
attracted
are
more
numerous,
viable
with
higher
integrity.
In
addition,
fertilized
eggs,
but
if
this
is
due
fertilization
ability
per
se
or
numerical
advantage
remains
be
tested.
Our
results
suggest
select
better
phenotype,
highlighting
crucial
impactful
role
might
play
process
post-mating
sexual
dynamics,
along
implications
assisted
techniques.
Journal of Animal Science,
Journal Year:
2024,
Volume and Issue:
102
Published: Jan. 1, 2024
Abstract
This
study
used
a
porcine
model
to
systematically
investigate
whether
carboxyfullerene
C60(CF-C60)
can
be
for
sperm
preservation.
The
results
indicated
that
CF-C60
supplementation
preserve
quality
during
storage
at
17
°C.
effect
was
attributable
an
improvement
in
the
antioxidant
capacity
of
through
decrease
reactive
oxygen
species
(ROS)
level.
Additionally,
maintain
mitochondrial
function,
inhibit
apoptosis
ROS/Cytochrome
C
(Cyt
C)/Caspase
3
signaling
pathway,
and
mediate
suppression
bacterial
growth
effects
ROS.
Finally,
artificial
insemination
experiments
with
CF-C60-treated
increase
total
number
offspring
born
reduce
deformed
piglets.
Thus,
is
safe
use
as
component
semen
diluent
storage.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: June 26, 2024
Japanese
medaka
(Oryzias
latipes)
has
been
used
as
a
model
organism
in
different
research
fields,
including
reproductive
physiology.
Sperm
motility
is
the
most
important
marker
for
male
fertility
fish
and,
thus,
reproduction
success.
However,
because
of
small
volume
ejaculate
and
short
duration,
it
still
challenging
to
manage
sperm
collection
analysis
fish.
In
present
study,
we
aimed
investigate
optimize
collection,
short-term
storage,
cryopreservation
latipes).
Using
two
approaches
collection:
testes
dissection
abdominal
massage,
housing
conditions
activating
with
activation
solutions,
investigated
immediate
motility.
second
part
this
osmolalities
immobilization
solution,
Hank's
Balanced
Salt
Solution
(HBSS)
storage
at
0,
2
3
h
after
collection.
Finally,
were
cryopreserved
using
methanol
cryoprotectant
HBSS
extender
osmolalities,
post-thaw
was
investigated.
The
highest
post-activating
achieved
groups
activated
by
300
mOsm/kg.
quality
remained
unaffected
co-housing
females
or
males
only.
Furthermore,
Hanks'
an
osmolality
600
mOsm/kg
demonstrated
its
efficacy
suitable
preserving
progressivity
h.
around
35%.
There
no
significant
differences
between
groups.
We
also
found
that
incubation
on
ice
can
maintain
up
one
hour
thawing.
