Sperm quality and function deteriorate in old males of the naturally short-lived turquoise killifish DOI Creative Commons
Silvia Cattelan, Dario Riccardo Valenzano

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 14, 2023

Maximizing the life-long reproductive output would lead to prediction that short-lived and fast aging species undergo no - if any senescence. Turquoise killifish (Nothobranchius furzeri) are naturally teleosts, extensive somatic aging, characterized by molecular, cellular, organ dysfunction following onset of sexual maturation. Here, we tested whether turquoise males maximize reproduction display minimal any, We analysed age-related changes in sperm traits, proportion fertilized eggs, as well embryo survival. Contrary expectation found consisting age-dependent decline quality, decreased eggs lower Our results indicate male do not trade-off soma with sustained fitness. Instead, appear occur simultaneously. findings question generalized vs. senescence models highlight importance integrating species-specific ecological demographic constraints explain observed life history traits.

Language: Английский

Pre-but not post-meiotic senescence affects sperm quality and reproductive success in the North African houbara bustard DOI Creative Commons
Léna Meunier, Gabriele Sorci,

Hiba Abi Hussein

et al.

Frontiers in Ecology and Evolution, Journal Year: 2022, Volume and Issue: 10

Published: Dec. 8, 2022

Age-dependent reduction in reproductive success can arise due to multiple factors including a deterioration of physiology. Senescing males have been shown produce ejaculates with poor sperm quality, which impinges on male success. In addition individual age, gamete age also affect Accordingly, variance be pre-meiotic (referring age) and post-meiotic senescence (sperm age). Here, we tested whether cell aging additive or interactive effects bird promiscuous mating system, the North African houbara bustard. To assess effect aging, compared between two classes (3-6- 12–16-year-old). infer were collected at three-time intervals following common initial collection (day 1, 5, 10). Therefore, day 1 are supposed contain younger than 5 10 ejaculates. Following controlled artificial inseminations, was assessed using three fitness-linked traits (hatching success, chick growth rate survival). output, pre- affected wide range ejaculate traits. agreement previous reports, found that older class produced less poorer motility young individuals. However, contrary prediction, tended better such as velocity. The results generally mirrored since offspring grew faster had survival during first month life, eggs fertilized by highest hatching any models, there evidence for age. Overall, these confirm role lack could come from experimental design but might reflect pattern frequency species lek-based system.

Language: Английский

Citations

6
Female Reproductive Fluid Increases the Opportunities for Postmating Sexual Selection by Prolonging the Egg Fertilization Window DOI
Livia Pinzoni, Federica Poli, Alessandro Grapputo

et al.

The American Naturalist, Journal Year: 2022, Volume and Issue: 201(3), P. 491 - 499

Published: Oct. 4, 2022

AbstractFemale reproductive fluid (the that surrounds the eggs) has attracted increasing attention for its role in fertilization and postmating sexual selection through effects on sperm traits. Surprisingly, however, only a few studies have investigated of female eggs. Yet these might offer great potential to affect dynamics by, example, opportunities selection. Here, we determined whether fluid, by extending egg window time available fertilization), could also increase multiple paternity. Using zebrafish (Danio rerio), first tested prediction increases window; then, using split-brood design with two males added at different points after activation, degree paternity varies presence or absence fluid. Our results reveal window, thus broadening our knowledge how mechanisms externally fertilizing species.

Language: Английский

Citations

6
Separating the effects of paternal age and mating history: Evidence for sex‐specific paternal effect in eastern mosquitofish DOI Creative Commons
Upama Aich, Shawan Chowdhury, Michael D. Jennions

et al.

Evolution, Journal Year: 2022, Volume and Issue: 76(7), P. 1565 - 1577

Published: May 11, 2022

Paternal age and past mating effort by males are often confounded, which can affect our understanding of a father's effects. To knowledge, only few studies have standardized history when testing for effects paternal age, none has simultaneously disentangled how might jointly influence offspring traits. Here, we experimentally manipulated male to tease apart its from those on female fertility traits in the eastern mosquitofish (Gambusia holbrooki). Male did not fertility. However, with greater produced significantly larger broods. interacted sons' body size: sons sired old-virgin were than old-mated males, but this was case younger fathers. Intriguingly, however, tended produce fewer sperms indicating potential trade-off beneficial Finally, neither nor affected daughter's fitness. Our results highlight that variation attributed effect could partly arise due history, simply his chronological age.

Language: Английский

Citations

5
Noninvasive Ejaculate Collection from the African Turquoise KillifishNothobranchius furzeri DOI
Silvia Cattelan, Dario Riccardo Valenzano

Cold Spring Harbor Protocols, Journal Year: 2023, Volume and Issue: 2023(5), P. pdb.prot108155 - pdb.prot108155

Published: Feb. 13, 2023

Ejaculate collection from live animals is a fundamental technique used to perform repeated measures on the same individual-for instance, test effect of different treatments/conditions animal or conduct longitudinal studies. Moreover, collecting ejaculate allows one in vitro fertilizations (IVFs) sperm cryopreservation valuable individuals (e.g., mutant, transgenic animals) without need kill animals. We developed noninvasive protocol based stripping procedure for males African turquoise killifish (Nothobranchius furzeri). The proposed retrieval mature along with seminal fluid. can be within 1 h and/or quality measurements. Contamination by mucus, water, urine, feces activate negatively affect quality, so special care needed avoid contamination and thus prolong viability. Since small fish amount collected limited few microliters, present we also provide recommendations housing conditions maximize probability obtain samples.

Language: Английский

Citations

2
Modeling of cryopreservation pathway operation at an aquatic biomedical stock center for zebrafish DOI Creative Commons
Sarah Bodenstein, Fidan Abdullayeva,

Joy M. Murphy

et al.

Cryobiology, Journal Year: 2023, Volume and Issue: 113, P. 104792 - 104792

Published: Nov. 7, 2023

Language: Английский

Citations

2
Carboxyfullerene C60 preserves sperm by enhancing antioxidant capacity and inhibiting apoptosis and harmful bacteria DOI Creative Commons
Yuqing Li,

H. Zhang,

Haoqi Xiao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

Abstract Background The current approaches for the preservation of human sperm have several limitations, and there are a lack effective non-freezing methods. Recently, carboxyfullerene C 60 (CF-C ) has gained attention as an important nanocarbon derivative with strong antioxidant antibacterial activity. This study uses porcine model to systematically investigate whether CF-C can be used sperm. Results results indicate that supplementation preserve quality during storage at 17°C. effect is attributable improvement in capacity through decrease ROS level. Additionally, maintain mitochondrial function, inhibit apoptosis ROS/Cytochrome C/Caspase 3 signaling pathway, mediate suppression bacterial growth effects ROS. Finally, artificial insemination experiments -treated increase total number offspring born reduce deformed piglets. Conclusions Thus, by inhibition via reduction levels safe use component semen diluent storage. These findings pave way prospective clinical application carbon nano-materials antioxidants

Language: Английский

Citations

0
High temperature induces motility of zebrafish sperm after short-term storage DOI Creative Commons
Songpei Zhang, Swapnil Gorakh Waghmare, Sayyed Mohammad Hadi Alavi

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 595, P. 741623 - 741623

Published: Sept. 13, 2024

Language: Английский

Citations

0
Sperm quality metrics exhibit annual fluctuations in a critically endangered amphibian managed under human care DOI Creative Commons
Allison R. Julien, Isabella J. Burger,

Carrie K. Kouba

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

Abstract The Puerto Rican crested toad (PRCT) is Rico’s only endemic and has undergone rapid population decline within the last 40 years. As a hedge against extinction, captive assurance colonies have been established at several zoological institutions for breeding reintroduction. However, reproductive output remained low, despite use of hormone therapies to attempt bypass missing abiotic cues stimulate behaviors. This low necessitates better understanding natural fluctuations in gamete, specifically sperm, quality individuals. To generate an gametic cycles PRCTs, we administered male PRCTs (n = 86) housed under temperature photoperiod with exogenous hormones monthly one year. Samples were analyzed motility concentration assess variations sperm by month. Spermiation was successfully stimulated every month, but fluctuated; highest June July, while December, January, March. These results indicate that, can be utilized gamete production year-round, not consistent. Furthermore, seasonal occurrence peak males differed from peaks reported wild PRCTs. Our illustrate need more biologically informed strategies at-risk anuran populations.

Language: Английский

Citations

0
End of the Season Blues: Senescence and Reproductive Trade-Offs in Male Scorpions DOI Creative Commons
David E. Vrech, Mariela A. Oviedo‐Diego,

Paola A. Olivero

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 916 - 916

Published: Nov. 23, 2024

Seasonal reproductive dynamics and senescence have profound impacts on male fertility, yet these processes remain understudied in scorpions. This study investigates how parameters-such as testicular mass, sperm quantity, viability-change over the course of season

Language: Английский

Citations

0
Age‐related changes in male ant sperm quality DOI
Félicien Degueldre, Serge Aron

Journal of Zoology, Journal Year: 2023, Volume and Issue: 321(1), P. 33 - 39

Published: June 15, 2023

Abstract Sperm cells age during storage in the male reproductive organs, which can lead ejaculate quality to decline dramatically over time. Such may pose a particular concern species with constrained sperm supplies, where adult males cannot replenish their reserves. In eusocial hymenopterans (i.e . ants, bees and wasps permanent castes), typically reach adulthood limited quantity of sperm, testes deteriorate shortly thereafter. life, both sexes mate single event. While die quickly after copulation, they persist posthumously via stored mates' spermatheca, sometimes for decades. As result this mating system, should experience intense selection pressure produce transfer high‐quality sperm. Using black garden ant ( Lasius niger ) as model we investigated whether duration accessory before copulation affected quality. We found that there was no impact on number or viability DNA fragmentation decreased rather increased age. These results highlight extent possibly other taxa have evolved extremely specialized strategies ensure performance.

Language: Английский

Citations

1