Improving the Production of Secondary Metabolites via the Application of Biogenic Zinc Oxide Nanoparticles in the Calli of Delonix elata: A Potential Medicinal Plant

Metabolites, Journal Year: 2023, Volume and Issue: 13(8), P. 905 - 905

Published: Aug. 2, 2023

The implementation of nanotechnology in the field plant tissue culture has demonstrated an interesting impact on vitro growth and development. Furthermore, accompanying nanoparticles been showed to be a reliable alternative for biosynthesis secondary metabolites. Herein, effectiveness zinc oxide (ZnONPs) Delonix elata calli, as well their phytochemical profiles, were investigated. seeds collected germinated, then species was determined based PCR product sequence ITS1 ITS4 primers. Afterward, calli derived from seedlings subjected 0, 10, 20, 30, 40, 50 mg/L ZnONPs. ZnONPs biologically synthesized using Ricinus communis aqueous leaf extract, which acts capping reducing agent, nitrate solution. nanostructures biogenic confirmed different techniques like UV-visible spectroscopy (UV), zeta potential measurement, Fourier transform infrared spectra (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM). Adding 30 MS media (containing 2.5 µM 2,4-D 1 BAP) resulted highest callus fresh weight (5.65 g) compared control other ZnONP treatments. Similarly, more phenolic accumulation (358.85 µg/g DW) flavonoid (112.88 contents achieved at mg/L. high-performance liquid chromatography (HPLC) analysis significant increments gallic acid, quercetin, hesperidin, rutin all treated control. On hand, gas mass (GC-MS) extracts revealed that nine compounds common among extracts. Moreover, most predominant compound found with bis(2-ethylhexyl) phthalate, percentage areas 27.33, 38.68, 22.66, 17.98%, respectively. 10 octadecanoic 2-propenyl ester heptanoic acid. In conclusion, this study, green exerted beneficial effects improved production bioactive compounds, especially dose

Language: Английский

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Fabrication of Algogenic Zinc Nanoparticles and Assessment of Their Biomimetics Attributes and Potential Antibacterial Efficacy Against Fish Pathogens

Aquaculture Research, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

This study investigates the fabrication of algogenic zinc nanoparticles (ZnNPs), examining their biomimetic properties and potential antibacterial effectiveness against fish pathogens. The rising incidence antibiotic resistance in aquaculture demands innovative strategies for disease management, highlighting importance developing biocompatible effective antimicrobial agents. In this context, ZnNPs were synthesized using Pediastrum boryanum microalgal extract as reducing capping agent. Transmission electron microscopy (TEM) revealed spherical tetragonal particles with sizes ranging from 32.84 to 26.87 nm. Fourier transform infrared spectroscopy (FTIR) demonstrated distinctive functional groups algal extracts ZnNPs. X‐ray diffraction (XRD) showed pure crystallinity fabricated an average crystalline size 57.97 had high total phenolic (TPC) flavonoid (TFC) contents (TPC: 53.83 TFC: 12.44 mg QE/g, respectively). obtained retained a considerable amount TPC TFC (7.92 5.31 mg/g, exhibited antioxidant capacity 2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging assay. addition, they elicited efficacy several bacterial pathogens minimum inhibitory concentration (MIC) Brine shrimp lethality assay (BSLA) higher cytotoxicity biosynthesized at 29.28 μg/mL compared that 51.56 μg/mL. These findings highlight P. green factory nanofabrication metallic (NPs) promising clinical candidate medicine.

Language: Английский

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Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 26, 2022

Bioactive compounds of medicinal plants present as natural ingredients provide health benefits beyond the basic nutritional value these products. However, availability bioactive in current sources is limited. Hence, induction compound production from through nanoparticles (NPs) might play a vital role industrially important compounds. Therefore, this study aimed to synthesize silver (AgNPs) biologically and investigate their effect on phytochemical callus Juniperus procera. AgNPs were synthesized using aqueous leaf extract Phoenix dactylifera, which acted reducing capping agent, nitrate solution. The formation has been confirmed different analytical techniques such UV-Visible spectroscopy (UV), Fourier-transform infrared (FTIR), dynamic light scattering (DLS), scanning electron microscope (SEM). impact concentrations (0.0, 5, 20, 50 mg/L) enzymatic non-enzymatic antioxidants J. procera was investigated. obtained results showed significant biomass accumulation (phenol, tannin, flavonoid content). Additionally, total protein content superoxide dismutase (SOD) activity increased response AgNPs. Furthermore, like gallic acid, tannic coumarin, hesperidin, rutin, quercetin, ferruginol chromatographically separated quantified high-performance liquid chromatography (HPLC) with reference standards. These significantly treatments. We concluded that could be promising elicitor for improving plants. This work can serve asa good model vitro. molecular investigation should done understand better metabolic mechanism leading scaling.

Language: Английский

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Foliar application of biosynthesised zinc nanoparticles as a strategy for ferti-fortification by improving yield, zinc content and zinc use efficiency in amaranth

Heliyon, Journal Year: 2022, Volume and Issue: 8(10), P. e10912 - e10912

Published: Oct. 1, 2022

Deficiency in zinc is widely prevalent developing countries. Ferti-fortification one of the easiest and quickest options for improving content food. Consumption such food can provide adequate amounts to individual. Nanotechnology now envisioned as future agriculture owing immense advantages nanoparticles over bulk materials. In this work, effect (Nps) synthesized via biological route using moringa leaves extract was studied on seed germination, its growth parameters, nutrient use efficiency amaranth crop. Moringa are rich plant metabolites amino acids, alkaloids, flavonoids, sugars fatty acids confirmed by UPLC-MS system analysis. The XRD studies show that biosynthesized Nps were hexagonal crystals with an average size 23.69 nm. particle indicated scanning electron microscopy between 15 30 nm, DLS 22.8 Foliar application 10 ppm Nps, resulted highest height fresh weight. Although, increase concentration applied through foliar led higher biomass, indices at better recovery, improved yield productivity respect input. This reflects advantage biologically counterparts. These results be attractive alternative conventional fertilizers biofortification crop yields.

Language: Английский

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Metal oxide nanoparticles and plant secondary metabolism: unraveling the game-changer nano-elicitors

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2023, Volume and Issue: 155(2), P. 327 - 344

Published: Aug. 14, 2023

Language: Английский

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