The cervical microbiota of Hispanics living in Puerto Rico is nonoptimal regardless of HPV status

mSystems, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 3, 2023

ABSTRACT The cervicovaginal microbiota is influenced by host physiology, immunology, lifestyle, and ethnicity. We hypothesized that there would be differences in the among pregnant, nonpregnant, menopausal women living Puerto Rico (PR) with without human papillomavirus (HPV) infection cervical cancer. specifically wanted to determine if associated variations cytology. A total of 294 women, including reproductive-age nonpregnant ( N = 196), pregnant 37), 61) were enrolled. bacteria characterized 16S rRNA amplicon sequencing, HPV was genotyped SPF10-LiPA, cytology quantified. High-risk (HR-HPV, 67.3%) prevalent, genotypes not covered 9vt vaccine. Cervical lesions (34%) also common. dominated Lactobacillus iners . Pregnant second third trimesters exhibited a decrease diversity abundance microbes bacterial vaginosis. Women menopause had greater alpha diversity, proportion facultative strictly anaerobic bacteria, higher pH than premenopausal women. diversity. However, no significant associations between (HR or LR-HPV types) found. Rican either L. diverse microbial communities regardless woman’s physiological stage. postulate high prevalence HR-HPV increase risk PR. IMPORTANCE In enclosed manuscript, we provide first in-depth characterization Hispanic (PR), using approach, include different stages. Surprisingly found high-risk ubiquitous 67.3%, types highly across groups—with reduction but nonoptimal Additionally, vaginosis-associated as Dialister spp., Gardnerella Clostridium , Prevotella most believe this relevant timely article expanding knowledge on microbiome PR where these are conducive disease.

Language: Английский

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Dynamics of the species composition of vaginal microorganisms in women before delivery and at different times of the postpartum period

Journal of obstetrics and women s diseases, Journal Year: 2025, Volume and Issue: 73(6), P. 162 - 171

Published: Jan. 30, 2025

Background: Vaginal microbiota is a factor that determines woman’s health. Infectious complications of pregnant women, women in labor and newborns are often associated with significant change its composition. Analysis the species diversity vaginal during pregnancy postpartum period primarily contributes to study physiological processes concept “healthy” environment these periods life. Aim: The aim this was evaluate dynamic composition microorganisms before delivery at different times natural labor. Materials methods: This involved 24 who delivered vaginally. Each patient examined three time points: 37–40 weeks (visit 1), 4–5 days 2), 6–8 after 3). studied using comprehensive test based on real-time quantitative polymerase chain reaction. Results: We found decrease total bacterial mass biotope comparison examination (p 0.0005). Similar changes were noted lactobacillary microbiota: concentration lactobacilli decreased 0.05). Compared delivery, we observed frequency Lactobacillus crispatus dominance 0.05), while iners increased Among representatives opportunistic microflora, most frequently prevalent Gardnerella vaginalis, Prevotella bivia, Porphyromonas spp., Fannyhessea vaginae, Staphylococcus Streptococcus Ureaplasma spp. In all severe disruption an ascending infection established according histological placenta Conclusions: cases, qualitative physiological. At same time, consistent will allow for identifying possible risk factors development infectious diseases mother newborn expand possibilities timely diagnosis treatment identified disorders.

Language: Английский

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Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 3, 2025

Language: Английский

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Metagenomic investigation of 16S rRNA marker gene samples to analyze the role of race, ethnicity, and location in preterm birth: A comprehensive vaginal microbiome meta-analysis

Human Gene, Journal Year: 2024, Volume and Issue: 39, P. 201260 - 201260

Published: Jan. 8, 2024

Language: Английский

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Biomarker Identification for Preterm Birth Susceptibility: Vaginal Microbiome Meta‐Analysis Using Systems Biology and Machine Learning Approaches

American Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 92(1)

Published: July 1, 2024

ABSTRACT Problem The vaginal microbiome has a substantial role in the occurrence of preterm birth (PTB), which contributes substantially to neonatal mortality worldwide. However, current bioinformatics approaches mostly concentrate on taxonomic classification and functional profiling microbiome, limiting their abilities elucidate complex factors that contribute PTB. Method study A total 3757 16S rRNA samples were obtained from five publicly available datasets. divided into two categories based pregnancy outcome: (PTB) ( N = 966) term 2791). Additionally, further categorized participants’ race trimester. reads subjected using Parallel‐META 3 software Ubuntu environment. abundances analyzed an integrated systems biology machine learning approach determine key microbes, pathways, genes resulting features statistical analysis identify top nine with greatest effect sizes. Results We identified significant features, namely Shuttleworthia , Megasphaera Sneathia proximal tubule bicarbonate reclamation pathway, systemic lupus erythematosus transcription machinery lepA gene, pepX rpoD gene. Their abundance variations observed through trimesters. Conclusions Vaginal infections caused by altered small metabolite biosynthesis pathways such as lipopolysaccharide folate retinal may increase susceptibility organisms, genes, networks be specifically targeted for treatment bacterial PTB risk.

Language: Английский

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Is there any difference in Lactobacillus abundance between age groups during ovarian stimulation in fresh in vitro fertilization (IVF) cycles?

Medicinski Glasnik, Journal Year: 2024, Volume and Issue: 21(2), P. 338 - 343

Published: Sept. 1, 2024

<p><strong>Aim</strong> To investigate an influence of age and body mass index (BMI) as determinants infertility treatment success in vitro fertilization (IVF) procedures confirmed so far, on the amount <em>Lactobacillus</em> spp. (LS) <em>Gardnerella vaginalis</em> (GV) endometrium fresh IVF cycles at time embryo transfer.<br /><strong>Methods</strong> This a prospective single-centre study included patients who underwent transfer (ET) private Centre. A catheter tip for was used collecting samples from endometrial cavity. Real polymerase chain reaction (RT PCR) to determine LS GV quantity. Patients were divided into two groups: <35 years ≥35 years. Normal BMI group with 18.-24.9 kg/m<sup>2</sup>, in-creased ≥ 25kg/m<sup>2</sup>.<br /><strong>Results</strong> Tips catheters positive 55 (96.4%) patients. The median quantification cycle RT-PCR (Cq RT-PCR) 33, 31 younger older groups, respectively. Twenty-one (36.8%) tested GV. Cq 33 34 There 36 normal 18 increased BMI. showed no correlation cavity.<br /><strong>Conclusion</strong> Aging affects endome-trial microbiome resulting increase number, but not number GV.</p>

Language: Английский

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Microbiome in Defence Against Pathogens

Published: Jan. 1, 2024

Language: Английский

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