LC–MS/MS, GC–MS and molecular docking analysis for phytochemical fingerprint and bioactivity of Beta vulgaris L.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 29, 2024

Abstract The plants that we consume in our daily diet and use as a risk preventer against many diseases have biological pharmacological activities. In this study, the phytochemical fingerprint activities of Beta vulgaris L. leaf extract, which are widely consumed Black Sea region, were investigated. parts plant dried an oven at 35 °C then ground into powder. main constituents B. identified by LC–MS/MS GC–MS analyses. Phenolic content, betaxanthin betacyanin levels investigated extracts obtained using three different solvents. activity extract was anti-microbial, anti-mutagenic, anti-proliferative anti-diabetic tests. Anti-diabetic vitro enzyme inhibition in-silico molecular docking performed to confirm activity. LC–MS analysis major proportion p_coumaric acid, vannilin, protecatechuic aldehyde sesamol detected, while essential oils determined hexahydrofarnesyl acetone phytol. Among solvents used, highest extraction efficiency 2.4% methanol extraction, 36.2 mg GAE/g phenolic substance, 5.1 mg/L 4.05 extract. , exhibited broad-spectrum anti-microbial forming zone all tested bacteria, anti-mutagenic range 35.9–61.8% various chromosomal abnormalities. did not exhibit mutagenic, sub-lethal or lethal effects, reducing proliferation Allium root tip cells 21.7%. 50 mg/mL caused 58.9% 55.9% α-amylase α-glucosidase activity, respectively. interactions coumaric vanniline, phytol, compounds with silico between molecules via amino acids determined. Binding energies − 4.3 kcal/mol 6.1 kcal/mol, for it 3.7 5.7 kcal/mol. closely related active contains, therefore studies investigating contents very important. Safe non-toxic can help reduce diseases, such diabetes, serve alternative complement current pharmaceutical practices.

Language: Английский

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Investigating the Discoloration of Leaves of Dioscorea polystachya Using Developed Atomic Absorption Spectrometry Methods for Manganese and Molybdenum

Molecules, Journal Year: 2024, Volume and Issue: 29(16), P. 3975 - 3975

Published: Aug. 22, 2024

The Chinese yam (Dioscorea polystachya, DP) is promising for the food and pharmaceutical industries due to its nutritional value potential. Its proper cultivation therefore of interest. An insufficient supply minerals necessary plant growth can be manifested by discoloration leaves. In our earlier study, magnesium deficiency was excluded as a cause. As follow-up, this work focused on manganese molybdenum. To quantify both in leaf extracts DP, analytical methods based atomic absorption spectrometry (AAS) using graphite furnace sub-technique were devised. development revealed that quantification works best without any investigated modifiers. optimized pyrolysis atomization temperatures 1300 °C 1800 °C, respectively. For analysis molybdenum, calcium proved advantageous modifier. optimum 1900 2800 Both showed satisfactory linearity analysis. Thus, they applied from normal discolored leaves DP concerning two minerals. It found had higher levels lower molybdenum content. With these results, potential explanation could found.

Language: Английский

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LC–MS/MS phenolic profileand remedial role of Urtica dioica extract against Li2CO3-induced toxicity

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(42), P. 54589 - 54602

Published: Aug. 29, 2024

Language: Английский

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A study on the effect of Hypericum perforatum L. extract on vanadium toxicity in Allium cepa L.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 18, 2024

The growth of industrialization the risk vanadium (V) contamination. objective this study was to examine impact 200 µg L

Language: Английский

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Effect of formaldehyde exposure on phytochemical content and functional activity of Agaricus bisporus (Lge.) Sing.

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(24), P. 35581 - 35594

Published: May 11, 2024

In this study, the effect of formaldehyde on phytochemical content and antioxidant activity Agaricus bisporus was investigated. Synthetic compost based wheat straw prepared by fermentation disinfection. After steam pasteurization, 5 g A. mycelia were inoculated into 1 kg compost. To determine effects formaldehyde, 2, 4, 6% concentrations added to composts, while without used for control group. The harvesting period set at 10 weeks. Total phenolic flavonoid content, macro- microelement profile, analyzed in harvested samples. Macro- determined ICP-OES, compound profile LC-MS/MS analysis. Formaldehyde levels samples acetylacetone spectrophotometry method. capacity DPPH scavenging activity; antimutagenic Allium test. Application resulted a 1.12-, 1.19-, 2.07-fold reduction total respectively. reduced between 34.4% 71.8%. These changes confirmed Compounds such as protocatechuic acid, salicylic ferulic 4-OH benzoic which detected group, could not be treated with it found that application content. Similar also observed microelements, significant elemental contents after application. While presence low level, may due natural production, residue 11.41 ± 0.93 mg/kg FMD applied All these decrease bisporus. activity, range 21.6-73.3% samples, decreased 12.3-56.7% formaldehyde. results indicate different stages cultivation leads nutritional value biological

Language: Английский

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Comparative analysis of cyto-genotoxicity of zinc using the comet assay and chromosomal abnormality test

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

Language: Английский

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Staggering cytotoxic effects of manganese oxide nanoparticles from Bacillus thuringiensis

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: unknown, P. 107184 - 107184

Published: Nov. 1, 2024

Language: Английский

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