ALKBH5 alleviates lower extremity arteriosclerosis by regulating ITGB1 demethylation and influencing macrophage polarization DOI Creative Commons
Zhiqiang Guan,

Xiaogao Wang,

Chao Xu

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 11(1), P. e41495 - e41495

Published: Dec. 31, 2024

M6A methylation-regulated macrophages play an important role in the occurrence and development of arteriosclerosis. However, their lower extremity arteriosclerosis remains unclear. Therefore, this study aims to explore key factors that regulate methylation extremities mechanism by which they affect influencing macrophage polarization. The m6A levels peripheral blood mononuclear cells (PBMCs) patients with atherosclerosis was investigated using Dot blot method. Additionally, expression RNA methyltransferases demethylases were examined ELISA Western blotting. Inflammatory established RAW264.7 stimulated LPS (100 ng/mL), ALKBH5 ITGB1 evaluated Immunofluorescence staining conducted assess M1 markers (F4/80+CD86) M2 (F4/80+CD206) renal tissue. employed measure cytokines (IL-6, IL-1β, TNF-a, IL-10, TGF-β) plasma lipid mice. An model ApoE-/- mice through balloon pull surgery high-fat feeding. Oil Red O hematoxylin eosin (HE) performed area atherosclerotic plaques size necrotic core mouse femoral artery, respectively. Starbase2.0 used for downstream target gene prediction ALKBH5. half-life RT-qPCR. significantly increased PBMCs atherosclerosis. Among them, demethylase lowest Further analysis revealed can alleviate progression promote polarization macrophages. reduce stability demethylation. Mechanistically, influences mitigates affecting demethylation ITGB1. promotes alleviates regulating A deeper understanding process not only helps elucidate molecular mechanisms but also provides possibilities new therapeutic strategies.

Language: Английский

WTAP Promotes Atherosclerosis by Inducing Macrophage Pyroptosis and M1 Polarization through Upregulating NLRP3 DOI
Xing Luo, Chaogui He, Bo Yang

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Language: Английский

Citations

0
Recent Advances in the Mutual Regulation of m6A Modification and Non-Coding RNAs in Atherosclerosis DOI Creative Commons

Yanlu Wang,

Shun M. Ling,

Hao Feng

et al.

International Journal of General Medicine, Journal Year: 2025, Volume and Issue: Volume 18, P. 1047 - 1073

Published: Feb. 1, 2025

Atherosclerosis, a progressive inflammatory disease of the arteries, remains leading cause cardiovascular morbidity and mortality worldwide. Recent years have witnessed pivotal role N6-methyladenosine (m6A) RNA methylation in regulating various biological processes, including those implicated atherosclerosis. Current evidence suggested that m6A regulators (writers, erasers, readers) participated modification multiple non-coding RNAs (ncRNAs), microRNAs (miRNAs), long (lncRNAs), circular (circRNAs), thereby affecting their metabolism functions. Meanwhile, ncRNAs also emerged as key modulator expression turn. Therefore, understanding mutual regulation between modifications is great significance to identify novel therapeutic targets for atherosclerosis has clinical application prospects. This review aims summarize recent advances reciprocal provide insights into interaction context

Language: Английский

Citations

0
Transcriptomic analysis reveals the function of m6A regulators in aged cochlea DOI

Yanbing Lai,

Bo Su, Xiaodi Wang

et al.

Brazilian Journal of Otorhinolaryngology, Journal Year: 2025, Volume and Issue: 91(3), P. 101578 - 101578

Published: April 8, 2025

Language: Английский

Citations

0
Epitranscriptomics in atherosclerosis: Unraveling RNA modifications, editing and splicing and their implications in vascular disease DOI Creative Commons
Victoria Stopa, Dimitra Dafou, Korina Karagianni

et al.

Vascular Pharmacology, Journal Year: 2025, Volume and Issue: 159, P. 107496 - 107496

Published: April 14, 2025

Language: Английский

Citations

0
Protective effect of vitexin against high fat-induced vascular endothelial inflammation through inhibiting trimethylamineN-oxide-mediated RNA m6A modification DOI
Wenwen Li, Zeyuan Deng,

Shuang Xiao

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(13), P. 6988 - 7002

Published: Jan. 1, 2024

A high-fat diet (HFD) is a major risk factor for cardiovascular disease.

Language: Английский

Citations

2
ALKBH5 alleviates lower extremity arteriosclerosis by regulating ITGB1 demethylation and influencing macrophage polarization DOI Creative Commons
Zhiqiang Guan,

Xiaogao Wang,

Chao Xu

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 11(1), P. e41495 - e41495

Published: Dec. 31, 2024

M6A methylation-regulated macrophages play an important role in the occurrence and development of arteriosclerosis. However, their lower extremity arteriosclerosis remains unclear. Therefore, this study aims to explore key factors that regulate methylation extremities mechanism by which they affect influencing macrophage polarization. The m6A levels peripheral blood mononuclear cells (PBMCs) patients with atherosclerosis was investigated using Dot blot method. Additionally, expression RNA methyltransferases demethylases were examined ELISA Western blotting. Inflammatory established RAW264.7 stimulated LPS (100 ng/mL), ALKBH5 ITGB1 evaluated Immunofluorescence staining conducted assess M1 markers (F4/80+CD86) M2 (F4/80+CD206) renal tissue. employed measure cytokines (IL-6, IL-1β, TNF-a, IL-10, TGF-β) plasma lipid mice. An model ApoE-/- mice through balloon pull surgery high-fat feeding. Oil Red O hematoxylin eosin (HE) performed area atherosclerotic plaques size necrotic core mouse femoral artery, respectively. Starbase2.0 used for downstream target gene prediction ALKBH5. half-life RT-qPCR. significantly increased PBMCs atherosclerosis. Among them, demethylase lowest Further analysis revealed can alleviate progression promote polarization macrophages. reduce stability demethylation. Mechanistically, influences mitigates affecting demethylation ITGB1. promotes alleviates regulating A deeper understanding process not only helps elucidate molecular mechanisms but also provides possibilities new therapeutic strategies.

Language: Английский

Citations

0