Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 12, 2024
Waterborne
pathogens
are
harmful
microorganisms
transmitted
through
water
sources.
Early
and
rapid
pathogen
detection
is
important
for
preventing
illnesses
implementing
stringent
safety
measures
to
minimize
the
risk
of
contamination.
This
work
introduces
a
miniaturized
all-solid-state
potentiometric
ion
flux
immunosensor
label-free
waterborne
pathogens.
A
screen-printed
silver/silver
chloride
electrode
coated
with
reference
membrane
polyurethane
as
an
was
combined
solid-state
contact
ion-selective
(ISE).
An
ISE
constructed
on
laser-induced
graphene
by
coating
it
cationic
marker
carboxylated
poly(vinyl
chloride)-based
immobilizing
antibodies
controlling
fluxes
membrane.
Proof-of-concept
achieved
detecting
Laser-induced
graphene
(LIG)
has
emerged
as
a
promising
solvent-free
strategy
for
producing
highly
porous,
3D
structures,
particularly
electrochemical
applications.
However,
the
unique
character
of
LIG
and
hydrogel
membrane
(HM)
coated
requires
accounting
specific
conditions
its
charge
transfer
process.
This
study
investigates
electron
kinetics
electroactive
surface
area
electrodes,
finding
efficient
[Fe(CN)6]3-/4-
redox
process,
with
high
rate
constant
4.12
x
10-3
cm/s
an
28
times
higher
than
geometric
area.
The
impact
polysaccharide
HM
coatings
(cationic
chitosan,
neutral
agarose
anionic
sodium
alginate)
on
LIG's
behavior
is
elucidated,
considering
factors
like
Ohmic
drop
across
porous
Coulombic
interactions/permeability
affecting
diffusion
coefficients,
estimated
from
amperometry.
Experimental
findings
are
supported
by
ab-initio
calculations
showing
electrostatic
potential
map’s
negative
distribution
upon
chitosan
chain
protonation,
having
effect
in
over
two-fold
current
increase
switching
pH
7.48
to
1.73.
feature
absent
other
studied
HMs.
It
was
also
revealed
that
chitosan's
band
gap
reduced
3.07
eV
acetylation,
due
introduction
new
LUMO
state.
summarizes
operating
enhanced
presence,
impacting
process
presenting
challenges
prospective
LIG/HM
systems’
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 12, 2024
Waterborne
pathogens
are
harmful
microorganisms
transmitted
through
water
sources.
Early
and
rapid
pathogen
detection
is
important
for
preventing
illnesses
implementing
stringent
safety
measures
to
minimize
the
risk
of
contamination.
This
work
introduces
a
miniaturized
all-solid-state
potentiometric
ion
flux
immunosensor
label-free
waterborne
pathogens.
A
screen-printed
silver/silver
chloride
electrode
coated
with
reference
membrane
polyurethane
as
an
was
combined
solid-state
contact
ion-selective
(ISE).
An
ISE
constructed
on
laser-induced
graphene
by
coating
it
cationic
marker
carboxylated
poly(vinyl
chloride)-based
immobilizing
antibodies
controlling
fluxes
membrane.
Proof-of-concept
achieved
detecting
