Identification of Homeobox Transcription Factors in a Dimorphic Fungus Talaromyces marneffei and Protein-Protein Interaction Prediction of RfeB DOI Creative Commons
Monsicha Pongpom, Nopawit Khamto,

Panwarit Sukantamala

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(10), P. 687 - 687

Published: Sept. 30, 2024

Talaromyces marneffei is a thermally dimorphic fungus that can cause life-threatening systemic mycoses, particularly in immunocompromised individuals. Fungal homeobox transcription factors control various developmental processes, including the regulation of sexual reproduction, morphology, metabolism, and virulence. However, function proteins T. has not been fully explored. Here, we searched genome for total predicted their biological relevance by performing gene expression analysis different cell types, conidia, mycelia, yeasts, during phase transition. RfeB selected further computational since (i) its transcripts were differentially expressed phases marneffei, (ii) this protein contains highly conserved protein-protein interaction region (IR), which could be important pathobiology have therapeutic application. To assess structure-function IR region, silico alanine substitutions performed at three-conserved residues (Asp276, Glu279, Gln282) RfeB, generating triple mutated protein. Using 3D modeling molecular dynamics simulations, compared complex formation wild-type with putative partner candidate TmSwi5. Our results demonstrated exhibited increased free binding energy, elevated compactness, reduced number atomic contacts, suggesting disrupted stability interaction. Notably, our model revealed primarily stabilized sites located central (CR). This approach mutagenesis provide foundation future experimental studies on functional characterization other homeodomain-containing marneffei.

Language: Английский

Identification of Homeobox Transcription Factors in a Dimorphic Fungus Talaromyces marneffei and Protein-Protein Interaction Prediction of RfeB DOI Creative Commons
Monsicha Pongpom, Nopawit Khamto,

Panwarit Sukantamala

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(10), P. 687 - 687

Published: Sept. 30, 2024

Talaromyces marneffei is a thermally dimorphic fungus that can cause life-threatening systemic mycoses, particularly in immunocompromised individuals. Fungal homeobox transcription factors control various developmental processes, including the regulation of sexual reproduction, morphology, metabolism, and virulence. However, function proteins T. has not been fully explored. Here, we searched genome for total predicted their biological relevance by performing gene expression analysis different cell types, conidia, mycelia, yeasts, during phase transition. RfeB selected further computational since (i) its transcripts were differentially expressed phases marneffei, (ii) this protein contains highly conserved protein-protein interaction region (IR), which could be important pathobiology have therapeutic application. To assess structure-function IR region, silico alanine substitutions performed at three-conserved residues (Asp276, Glu279, Gln282) RfeB, generating triple mutated protein. Using 3D modeling molecular dynamics simulations, compared complex formation wild-type with putative partner candidate TmSwi5. Our results demonstrated exhibited increased free binding energy, elevated compactness, reduced number atomic contacts, suggesting disrupted stability interaction. Notably, our model revealed primarily stabilized sites located central (CR). This approach mutagenesis provide foundation future experimental studies on functional characterization other homeodomain-containing marneffei.

Language: Английский

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