Estimating SARS‐CoV‐2 Omicron XBB.1.5 Spike‐Directed Functional Antibody Levels From an Anti‐Receptor Binding Domain Wuhan‐Hu‐1‐Based Commercial Immunoassay Results DOI Creative Commons

Ángela Sánchez‐Simarro,

Enric Cuevas‐Ferrando, Daniel Fernández‐Soto

et al.

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(1)

Published: Jan. 1, 2025

ABSTRACT We investigated whether antibody concentrations measured in plasma using the Roche Elecsys® Anti‐SARS‐CoV‐2 S assay (targeting receptor binding domain, RBD) could estimate levels of Wuhan‐Hu‐1 and Omicron XBB.1.5 spike‐directed antibodies with neutralizing ability (NtAb) or those mediating NK‐cell activity. analyzed 135 samples from 39 vaccinated elderly nursing home residents. A strong correlation was found for NtAb against both (Rho = 0.73, p < 0.001) (sub)variants 0.001). Moderate positive correlations were observed activity, based on lysosome‐associated membrane protein 1 (LAMP1)‐producing NK cells stimulated 0.43, spike proteins 0.50, Similarly, interferon‐gamma (IFN‐γ)‐producing frequencies showed moderate (Wuhan‐Hu‐1: Rho 0.001; XBB.1.5: Random Forest models accurately predicted ( R 2 0.72), though less robust. Anti‐RBD 4.73 5.02 log 10 BAU/mL high XBB.1.5, respectively. Antibody thresholds predicting functional cell‐mediated responses 4.54 For LAMP1‐producing cells, 4.94 4.75 In summary, total anti‐RBD by may allow inference and, to a lesser extent, Fc‐mediated XBB.1.5.

Language: Английский

Estimating SARS‐CoV‐2 Omicron XBB.1.5 Spike‐Directed Functional Antibody Levels From an Anti‐Receptor Binding Domain Wuhan‐Hu‐1‐Based Commercial Immunoassay Results DOI Creative Commons

Ángela Sánchez‐Simarro,

Enric Cuevas‐Ferrando, Daniel Fernández‐Soto

et al.

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(1)

Published: Jan. 1, 2025

ABSTRACT We investigated whether antibody concentrations measured in plasma using the Roche Elecsys® Anti‐SARS‐CoV‐2 S assay (targeting receptor binding domain, RBD) could estimate levels of Wuhan‐Hu‐1 and Omicron XBB.1.5 spike‐directed antibodies with neutralizing ability (NtAb) or those mediating NK‐cell activity. analyzed 135 samples from 39 vaccinated elderly nursing home residents. A strong correlation was found for NtAb against both (Rho = 0.73, p < 0.001) (sub)variants 0.001). Moderate positive correlations were observed activity, based on lysosome‐associated membrane protein 1 (LAMP1)‐producing NK cells stimulated 0.43, spike proteins 0.50, Similarly, interferon‐gamma (IFN‐γ)‐producing frequencies showed moderate (Wuhan‐Hu‐1: Rho 0.001; XBB.1.5: Random Forest models accurately predicted ( R 2 0.72), though less robust. Anti‐RBD 4.73 5.02 log 10 BAU/mL high XBB.1.5, respectively. Antibody thresholds predicting functional cell‐mediated responses 4.54 For LAMP1‐producing cells, 4.94 4.75 In summary, total anti‐RBD by may allow inference and, to a lesser extent, Fc‐mediated XBB.1.5.

Language: Английский

Citations

0