Aloe-emodin plus TIENAM ameliorate cecal ligation and puncture-induced sepsis in mice by attenuating inflammation and modulating microbiota DOI Creative Commons
Jingqian Su,

Xiaohui Deng,

Shan Hu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 12, 2024

Despite the high sepsis-associated mortality, effective and specific treatments remain limited. Using conventional antibiotics as TIENAM (imipenem cilastatin sodium for injection, TIE) is challenging due to increasing bacterial resistance, diminishing their efficacy leading adverse effects. We previously found that aloe-emodin (AE) exerts therapeutic effects on sepsis by reducing systemic inflammation regulating gut microbiota. Here, we investigated whether administering AE TIE post-sepsis onset, using a cecal ligation puncture (CLP)-induced model, extends survival improves physiological functions. Survival rates, inflammatory cytokines, tissue damage, immune cell populations, ascitic fluid microbiota, key signaling pathways were assessed. Combining significantly enhanced reduced load in septic mice, indicating potent antimicrobial properties. Moreover, substantial improvements rates of + TIE-treated mice (10% 60%) within 168 h observed relative CLP group. This combination therapy also effectively modulated marker (interleukin [IL]-6, IL-1β, tumor necrosis factor [TNF]-α) levels counts decreasing those B, NK, TNFR2+ T reg cells, while CD8+ cells; alleviated damage; peritoneal cavity; suppressed NF-κB pathway. altered cavity microbiota composition post-treatment, characterized pathogenic bacteria ( Bacteroides ) abundance. Our findings underscore potential treating sepsis, encourage further research possible clinical implementations surmount limitations amplify AE.

Language: Английский

Identifying key blood markers for bacteremia in elderly patients: insights into bacterial pathogens DOI Creative Commons
Shi‐Yan Zhang,

Ying Zhuo,

Bu-Ren Li

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 16, 2025

This study aimed to assess the distribution of bacteremia pathogens in elderly patients, examine impact gender on pathogen distribution, and evaluate predictive value routine blood parameters for diagnosing bacteremia. A retrospective analysis was conducted 151 patients (≥60 years old) admitted Fuding Hospital, Fujian University Traditional Chinese Medicine between October 2022 June 2023. Comprehensive tests cultures were performed. The diagnostic efficacy parameters, including white cell (WBC), neutrophil-to-lymphocyte ratio (NLR), platelet-lymphocyte (PLR), red width (RDW), evaluated using receive operating characteristic (ROC) curve analysis. Patients categorized into either culture-positive group (82 cases) or culture-negative (69 according culture results. No significant differences age found groups. primary bacterial Escherichia coli, Klebsiella pneumoniae Streptococcus. Elderly female demonstrated a significantly higher positivity rate E. coli compared their male counterparts (P = 0.021). areas under ROC (AUC) four as follows: WBC, 0.851 (95% confidence interval (CI) 0.790 - 0.912); NLR, 0.919 CI 0.875 0.963); PLR, 0.609 0.518 0.700); RDW 0.626 0.563 0.717). identified predominant pathogenic microorganism causing elderly, with among patients. Routine (WBC, RDW) potential

Language: Английский

Citations

1
Using random forest and biomarkers for differentiating COVID-19 and Mycoplasma pneumoniae infections DOI Creative Commons
Xun Zhou, Jie Zhang,

Xiu-Mei Deng

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 30, 2024

Language: Английский

Citations

3
Precision diagnostics of COVID-19 and Mycoplasma pneumoniae through random forest and biomarkers integration DOI
Xun Zhou, Jie Zhang,

Xiu-Mei Deng

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 16, 2024

Abstract The COVID-19 pandemic has highlighted the critical need for precise diagnostic methods to distinguish between similar respiratory infections such as and Mycoplasma pneumoniae (MP). Identifying key biomarkers utilizing machine learning techniques like Random Forest analysis can significantly improve accuracy. We conducted a retrospective of clinical laboratory data from 214 acute patients, categorized into three groups: positive (n = 52), MP-positive 140), co-infected with both 22). Key including C-reactive protein (CRP), procalcitonin (PCT), interleukin 6 (IL-6), white blood cell (WBC) counts were evaluated. algorithm was applied analyze discriminative power these biomarkers. Our revealed significant correlations age, PCT, IL-6, CRP, patient groups. model demonstrated high classification performance area under ROC curve (AUC) scores 0.91 COVID-19, 0.90 MP, 0.65 co-infections. Feature importance indicated that IL6, PCT most predictors. Confusion matrices Spearman correlation heatmaps model's accuracy relationships different integration computational methods, particularly analysis, in evaluating biomarker presents promising approach enhancing processes infectious diseases. findings support use specific differentiating potentially leading more targeted effective strategies. This study underscores potential improving disease era precision medicine.

Language: Английский

Citations

0
Aloe-emodin plus TIENAM ameliorate cecal ligation and puncture-induced sepsis in mice by attenuating inflammation and modulating microbiota DOI Creative Commons
Jingqian Su,

Xiaohui Deng,

Shan Hu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 12, 2024

Despite the high sepsis-associated mortality, effective and specific treatments remain limited. Using conventional antibiotics as TIENAM (imipenem cilastatin sodium for injection, TIE) is challenging due to increasing bacterial resistance, diminishing their efficacy leading adverse effects. We previously found that aloe-emodin (AE) exerts therapeutic effects on sepsis by reducing systemic inflammation regulating gut microbiota. Here, we investigated whether administering AE TIE post-sepsis onset, using a cecal ligation puncture (CLP)-induced model, extends survival improves physiological functions. Survival rates, inflammatory cytokines, tissue damage, immune cell populations, ascitic fluid microbiota, key signaling pathways were assessed. Combining significantly enhanced reduced load in septic mice, indicating potent antimicrobial properties. Moreover, substantial improvements rates of + TIE-treated mice (10% 60%) within 168 h observed relative CLP group. This combination therapy also effectively modulated marker (interleukin [IL]-6, IL-1β, tumor necrosis factor [TNF]-α) levels counts decreasing those B, NK, TNFR2+ T reg cells, while CD8+ cells; alleviated damage; peritoneal cavity; suppressed NF-κB pathway. altered cavity microbiota composition post-treatment, characterized pathogenic bacteria ( Bacteroides ) abundance. Our findings underscore potential treating sepsis, encourage further research possible clinical implementations surmount limitations amplify AE.

Language: Английский

Citations

0