Development and Validation of a Basement Membrane Inflammatory Response Gene Signature in Lung Adenocarcinoma DOI
Shilei Liu, Fei Liao,

Qingming Xie

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Abstract Inflammatory cell infiltration within the tumor microenvironment compromises basement membrane’ integrity, contributing to chemotherapy resistance and distant metastasis. This study investigates interplay between inflammatory responses membrane-related genes (BMIRGs) in lung adenocarcinoma (LUAD). With datasets from TCGA GEO databases, BMIRG risk-score model was developed based on univariate Cox regression a LASSO-Cox model. Nine were identified as comprising signature. Using this signature, we predictive models, including risk scores nomogram models. Stratifying patients into high-and low-risk groups revealed more favorable prognosis latter group. Additionally, signature exhibited correlations with drug sensitivity, immunotherapy response, of multiple immune cells factors microenvironment. The role one CCL20, further validated using intro experiments tissue samples Chinese LUAD. Functional that inhibiting CCL20 attenuated migration both A549 H1299 cells. Immunohistochemistry (IHC) Western blot (WB) analyses demonstrated protein levels significantly elevated LUAD tissues compared adjacent non-tumor also associated TNM stage. High expression linked shorter overall survival. In conclusion, is robust predictor survival offers valuable clinical tool for management. Moreover, emerges potential diagnostic therapeutic target, given its high association poorer prognosis.

Language: Английский

Elevated BEAN1 expression correlates with poor prognosis, immune evasion, and chemotherapy resistance in rectal adenocarcinoma DOI Creative Commons

Tiannake Shapaer,

Yi Chen,

Yipeng Pan

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 14, 2024

Language: Английский

Citations

15
Cancer‐Derived Extracellular Vesicle ITGB2 Promotes the Progression of Triple‐Negative Breast Cancer via the Activation of Cancer‐Associated Fibroblasts DOI Creative Commons
Jingjing Fan, Tong Sha,

Binlin Ma

et al.

Global Challenges, Journal Year: 2025, Volume and Issue: 9(3)

Published: Jan. 23, 2025

Abstract Breast cancer is the most prevalent and a leading cause of death among women globally, posing significant public health challenge. Triple‐negative breast (TNBC), an aggressive subtype accounting for 15–20% all cancers, lacks targeted therapies due to absence hormone receptors HER2, resulting in poor prognosis high recurrence rates. This study investigates role cancer‐derived extracellular vesicle (EV) integrin beta‐2 (ITGB2) TNBC progression. These findings reveal that ITGB2 significantly overexpressed tissues serum EVs, correlating with advanced tumor stages patient survival. enhances progression by activating cancer‐associated fibroblasts (CAFs) within microenvironment, promoting growth, migration, invasion. Mechanistic studies demonstrate EV facilitates CAF activation, driving tumor‐stroma interactions support results highlight as potential biomarker therapeutic target TNBC, emphasizing need novel interventions combat this challenging subtype.

Language: Английский

Citations

0
The role of tumor-associated macrophages in lung cancer DOI Creative Commons

Ruijuan Zhu,

Jing Huang,

Fenhong Qian

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Lung cancer remains a leading cause of cancer-related deaths worldwide, necessitating innovative treatments. Tumor-associated macrophages (TAMs) are primary immunosuppressive effectors that foster tumor proliferation, angiogenesis, metastasis, and resistance to therapy. They broadly categorized into proinflammatory M1 tumor-promoting M2 phenotypes, with elevated infiltration correlating poor prognosis. Strategies aimed at inhibiting TAM recruitment, depleting TAMs, or reprogramming therefore highly promising. Key signaling pathways, such as CSF-1/CSF-1R, IL-4/IL-13-STAT6, TLRs, CD47-SIRPα, regulate polarization. Additionally, macrophage-based drug delivery systems permit targeted agent transport hypoxic regions, enhancing Preclinical studies combining TAM-targeted therapies chemotherapy immune checkpoint inhibitors have yielded improved responses prolonged survival. Several clinical trials also reported benefits in previously unresponsive patients. Future work should clarify the roles macrophage-derived exosomes, cytokines, additional mediators shaping microenvironment. These insights will inform design next-generation carriers optimize combination immunotherapies within precision medicine frameworks. Elucidating phenotypes their regulatory molecules central developing novel strategies curb progression ultimately improve outcomes lung cancer. Importantly, immunomodulation may offer expanded treatment avenues.

Language: Английский

Citations

0
Transcriptome-based insights into the role of cancer-associated fibroblasts in lung adenocarcinoma prognosis and therapy DOI
Yinhe Feng, Jianming Zeng, Xiaoli Zhong

et al.

Computer Methods in Biomechanics & Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 16

Published: March 12, 2025

Cancer-associated fibroblasts (CAFs) are related to drug resistance and prognosis of tumor patients. This study aimed investigate the relationship between treatment response in patients with CAF lung adenocarcinoma (LUAD). The data pertaining LUAD were obtained from Cancer Genome Atlas-LUAD GSE68465 datasets. Four different algorithms used quantify infiltration stromal scores. Weighted gene network co-expression analysis was identify CAF-related modules hub genes. Univariate Cox regression analysis, least absolute shrinkage selection operator multivariate construct signatures, whose ability predict verified by individual signature eight genes constructed, score calculated. high scores significantly worse than that low an independent risk factor for prognosis. Patients sensitive some chemotherapy drugs, most cases, they non-responsive immunotherapy. Eight-gene may patient evaluate clinical responses immunotherapy, enabling individualized

Language: Английский

Citations

0
Development and Validation of a Basement Membrane Inflammatory Response Gene Signature in Lung Adenocarcinoma DOI
Shilei Liu, Fei Liao,

Qingming Xie

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Abstract Inflammatory cell infiltration within the tumor microenvironment compromises basement membrane’ integrity, contributing to chemotherapy resistance and distant metastasis. This study investigates interplay between inflammatory responses membrane-related genes (BMIRGs) in lung adenocarcinoma (LUAD). With datasets from TCGA GEO databases, BMIRG risk-score model was developed based on univariate Cox regression a LASSO-Cox model. Nine were identified as comprising signature. Using this signature, we predictive models, including risk scores nomogram models. Stratifying patients into high-and low-risk groups revealed more favorable prognosis latter group. Additionally, signature exhibited correlations with drug sensitivity, immunotherapy response, of multiple immune cells factors microenvironment. The role one CCL20, further validated using intro experiments tissue samples Chinese LUAD. Functional that inhibiting CCL20 attenuated migration both A549 H1299 cells. Immunohistochemistry (IHC) Western blot (WB) analyses demonstrated protein levels significantly elevated LUAD tissues compared adjacent non-tumor also associated TNM stage. High expression linked shorter overall survival. In conclusion, is robust predictor survival offers valuable clinical tool for management. Moreover, emerges potential diagnostic therapeutic target, given its high association poorer prognosis.

Language: Английский

Citations

0