A Single-Round Infectious Virus with a Codon-Deoptimized Genome: A Powerful Tool for Regulating Pathogenicity of Wild-Type/Live-Attenuated Viruses DOI
Takafumi Noguchi,

Anju Miyamori,

Takeshi Sugimoto

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Abstract Coxsackievirus B3 (CVB3) is a major cause of myocarditis and acute pancreatitis, particularly in neonates, whom infections result severe symptoms high mortality rates. Despite the urgent need for effective preventive strategies, no vaccines or therapeutic agents have been developed. Live-attenuated hold promise combating viral infections; however, their pathogenicity must be carefully regulated without compromising immunogenicity. Here, we investigated codon deoptimization defective genomes (DVGs) as strategies to modulate CVB3 pathogenicity, while preserving its immune-activating capacity. Codon-deoptimized CVB3s with increased CpG dinucleotide content 3CD region were engineered, leveraging innate immunostimulatory properties CpG. These modified exhibited attenuated proportional level induced protective immunity against wild-type (CVB3WT), making them viable live-attenuated vaccine candidates. Additionally, DVGs derived from codon-deoptimized demonstrated superior interference enhanced stimulation neutralizing antibody production compared CVB3WT. findings highlight that CpG-enriched are promising tools regulating enhancing safety, developing infections.

Language: Английский

Unlocking the potential of circular RNA vaccines: a bioinformatics and computational biology perspective DOI Creative Commons
Xuyuan Liu,

S H Wang,

Yunan Sun

et al.

EBioMedicine, Journal Year: 2025, Volume and Issue: 114, P. 105638 - 105638

Published: March 19, 2025

Language: Английский

Citations

0
A Single-Round Infectious Virus with a Codon-Deoptimized Genome: A Powerful Tool for Regulating Pathogenicity of Wild-Type/Live-Attenuated Viruses DOI
Takafumi Noguchi,

Anju Miyamori,

Takeshi Sugimoto

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Abstract Coxsackievirus B3 (CVB3) is a major cause of myocarditis and acute pancreatitis, particularly in neonates, whom infections result severe symptoms high mortality rates. Despite the urgent need for effective preventive strategies, no vaccines or therapeutic agents have been developed. Live-attenuated hold promise combating viral infections; however, their pathogenicity must be carefully regulated without compromising immunogenicity. Here, we investigated codon deoptimization defective genomes (DVGs) as strategies to modulate CVB3 pathogenicity, while preserving its immune-activating capacity. Codon-deoptimized CVB3s with increased CpG dinucleotide content 3CD region were engineered, leveraging innate immunostimulatory properties CpG. These modified exhibited attenuated proportional level induced protective immunity against wild-type (CVB3WT), making them viable live-attenuated vaccine candidates. Additionally, DVGs derived from codon-deoptimized demonstrated superior interference enhanced stimulation neutralizing antibody production compared CVB3WT. findings highlight that CpG-enriched are promising tools regulating enhancing safety, developing infections.

Language: Английский

Citations

0