Sex-specific effects of prenatal bisphenol A exposure on transcriptome-interactome profiles of autism candidate genes in neural stem cells from offspring hippocampus

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 22, 2025

Bisphenol A (BPA), an endocrine-disrupting chemical, is increasingly linked to the pathogenesis of autism spectrum disorder (ASD). This study investigates effects prenatal BPA exposure on neural stem cells (NSCs) from hippocampi rat offspring, a brain region critical for neurodevelopment and implicated in ASD. Pregnant rats were administered with or vehicle control once daily via oral gavage gestational day 1 until parturition. NSCs isolated offspring's postnatal 1, RNA sequencing was performed examine transcriptomic alterations. Differentially expressed genes (DEGs) identified through RNA-seq further analyzed using Ingenuity Pathway Analysis (IPA) explore disrupted pathways. In addition, vitro proliferation assays conducted, utilizing immunofluorescence staining Sox2, cell marker, BrdU quantify proliferating NSCs. Our results revealed that induced sex-specific alterations NSC gene expression, ASD-related such as Atp1a3, Nefl, Grin1 being particularly dysregulated male offspring. Moreover, changes observed. The underscores BPA's potential environmental risk factor ASD, emphasizing need research into its role neurodevelopmental effects.

Language: Английский

Neurodevelopmental impact of CNV models in ASD: Recent advances and future directions

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 92, P. 103001 - 103001

Published: March 15, 2025

Language: Английский