Immunostimulatory effects mechanism of polysaccharide extracted from Acanthopanax senticosus on RAW 264.7 cells through activating the TLR/MAPK/NF-κB signaling pathway DOI Creative Commons
Yufan Wang, Lei Wang, Hanwen Zhang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Acanthopanax senticosus is a unique wild resource in Northeast China. Its main active ingredient polysaccharide, which has prominent immunomodulatory effect. In this study, the purified polysaccharide component of leaves(ASPS-A1) with strong activity was isolated by column chromatography, and its structure properties were characterized HPGPC, FT-IR. Results showed that ASPS-A1 mainly composed α- 1,4-D-GalA, 1,5-L-Ara, β- 1,4-D-Gal. RT-qPCR experiments RNA-seq analysis used to study immunoregulatory mechanism ASPS-A1. The results could significantly up-regulate levels cytokines iNOS, IL- 1β, 6, TNF-α activated macrophages through MAPK, NF-κB, Toll-like receptor signaling pathways. Inhibitory further confirmed promotes expression TNF-α, 6 via TLR4 receptor, 1β TLR2 receptor. order identify target ASPS-A1, molecular docking conducted. demonstrated bind both TLR2, forming stable complexes cavities on protein surface hydrogen bonding hydrophobic interaction. scores indicated regulate immune response pathways, particularly interaction TLR4. summary, screened most immunoreactive components disclosed furnished research basis for potential application as natural enhancer.

Language: Английский

Preparation, structure and properties of litchi pericarp polysaccharide DOI Creative Commons
Yijie Wang, Gangliang Huang

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 21, 2025

Language: Английский

Citations

2
Chemical modification and antioxidant activity of Wendan peel polysaccharide DOI Creative Commons

Bobo Lin,

Gangliang Huang

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 20, 2025

Language: Английский

Citations

1
The antioxidant activity of shaddock peel polysaccharides DOI Creative Commons

Bobo Lin,

Gangliang Huang

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 21, 2025

The shaddock peel polysaccharide (SPP) has various biological activities. This explored its antioxidant activity. In vitro tests, the two SPP components showed good DPPH, hydroxyl radical scavenging ability and anti-lipid peroxidation ability. At a concentration of 3.2 mg/mL, abilities polysaccharides were 21.65% 18.96%, respectively. SPP-3 had slightly stronger to scavenge radicals than SPP-2. When reached order polysaccharides' DPPH rates was SPP-2 > SPP-3. Therefore, strong activity could be used in antioxidant-related applications future.

Language: Английский

Citations

0
Immunostimulatory effects mechanism of polysaccharide extracted from Acanthopanax senticosus on RAW 264.7 cells through activating the TLR/MAPK/NF-κB signaling pathway DOI Creative Commons
Yufan Wang, Lei Wang, Hanwen Zhang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Acanthopanax senticosus is a unique wild resource in Northeast China. Its main active ingredient polysaccharide, which has prominent immunomodulatory effect. In this study, the purified polysaccharide component of leaves(ASPS-A1) with strong activity was isolated by column chromatography, and its structure properties were characterized HPGPC, FT-IR. Results showed that ASPS-A1 mainly composed α- 1,4-D-GalA, 1,5-L-Ara, β- 1,4-D-Gal. RT-qPCR experiments RNA-seq analysis used to study immunoregulatory mechanism ASPS-A1. The results could significantly up-regulate levels cytokines iNOS, IL- 1β, 6, TNF-α activated macrophages through MAPK, NF-κB, Toll-like receptor signaling pathways. Inhibitory further confirmed promotes expression TNF-α, 6 via TLR4 receptor, 1β TLR2 receptor. order identify target ASPS-A1, molecular docking conducted. demonstrated bind both TLR2, forming stable complexes cavities on protein surface hydrogen bonding hydrophobic interaction. scores indicated regulate immune response pathways, particularly interaction TLR4. summary, screened most immunoreactive components disclosed furnished research basis for potential application as natural enhancer.

Language: Английский

Citations

0