Antivirulence Properties of Kuraridin Against Methicillin-Resistant Staphylococcus aureus (MRSA) DOI Creative Commons
Nilakshi Barua, Ben Chung-Lap Chan, Clara Bik‐San Lau

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(3), P. 564 - 564

Published: Feb. 24, 2025

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major human opportunistic pathogen that causes wide range of infections. The vast arsenal virulence factors expressed remains the biggest challenge in treating MRSA with conventional antibiotic therapy. Methods: We investigated effects Kuraridin at subinhibitory minimum inhibition concentrations (MICs) 1/8, 1/16, and 1/32 (concentrations did not inhibit bacterial growth) on adhesion to fibrinogen, adhesion, internalization into HaCaT cells, biofilm production three strains representing clonal types USA300, ST30, ST239. Results: All exhibited significant decrease (p < 0.001) fibrinogen upon treatment 1/8 1/16 MICs Kuraridin. all cells were decreased significantly formation USA300 0.001), ST30 ST239 0.01) was reduced MIC. A MIC observed for 0.05). Confocal laser scanning microscopy (CSLM) analysis biofilms revealed reduction strain when treated In vivo Caenorhabditis elegans model, offered sizable degree protection against infection without being toxic nematode. Conclusions: Our findings reveal has potential be an alternative antivirulence option reducing pathogenicity.

Language: Английский

Antivirulence Properties of Kuraridin Against Methicillin-Resistant Staphylococcus aureus (MRSA) DOI Creative Commons
Nilakshi Barua, Ben Chung-Lap Chan, Clara Bik‐San Lau

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(3), P. 564 - 564

Published: Feb. 24, 2025

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major human opportunistic pathogen that causes wide range of infections. The vast arsenal virulence factors expressed remains the biggest challenge in treating MRSA with conventional antibiotic therapy. Methods: We investigated effects Kuraridin at subinhibitory minimum inhibition concentrations (MICs) 1/8, 1/16, and 1/32 (concentrations did not inhibit bacterial growth) on adhesion to fibrinogen, adhesion, internalization into HaCaT cells, biofilm production three strains representing clonal types USA300, ST30, ST239. Results: All exhibited significant decrease (p < 0.001) fibrinogen upon treatment 1/8 1/16 MICs Kuraridin. all cells were decreased significantly formation USA300 0.001), ST30 ST239 0.01) was reduced MIC. A MIC observed for 0.05). Confocal laser scanning microscopy (CSLM) analysis biofilms revealed reduction strain when treated In vivo Caenorhabditis elegans model, offered sizable degree protection against infection without being toxic nematode. Conclusions: Our findings reveal has potential be an alternative antivirulence option reducing pathogenicity.

Language: Английский

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