Integrated transcriptomic and immune profiling reveals crucial molecular pathways and hub genes associated with postoperative delirium in elderly patients DOI Creative Commons

Zhuoyue Gou,

Nan Su, Xiaochuan Li

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: May 9, 2025

Background Postoperative delirium (POD) manifests as severe mental disorientation, often experienced by elderly patients undergoing surgery, significantly hindering recovery and deteriorating the quality of life. Despite numerous clinical studies, molecular mechanisms behind POD in are still not well understood, requiring further investigation to identify potential biomarkers therapeutic targets. Methods This study amalgamates Gene Set Variation Analysis (GSVA), Weighted Co-expression Network (WGCNA), differential expression analysis, immune infiltration assessments pathways hub genes linked initiation elderly. data were sourced from GSE163943 dataset Expression Omnibus (GEO) database. A total 18,894 protein-coding extracted for analysis. Results We constructed a gene co-expression network using WGCNA performed GSVA investigate link between different types cell death. The results indicated that is positively associated with pyroptosis parthanatos, while negatively correlated oxidative stress disulfidptosis. Differential analysis revealed 145 differentially expressed (DEGs), including 83 downregulated 62 upregulated genes. functional enrichment DEGs enriched activities like neuron projection development, axonogenesis, synapse organization, KEGG pathway identifying neuroactive ligand-receptor interaction neurodegeneration pathways. Enrichment (GSEA) upregulation apoptosis downregulation interaction. Protein–protein (PPI) identified 10 genes, COL18A1, CD63, LTF. Immune occurrence strongly activation, particularly T cells macrophages. Conclusion Overall, this research primarily examines intricate interplay death processes alterations microenvironment throughout development geriatric POD, pinpointing essential provide vital theoretical support studies on POD. However, discovery only an initial one derived analyzing datasets. Upcoming ought evaluate scrutinize additional datasets conduct experiments guarantee precision widespread relevance analytical findings.

Language: Английский

Integrated transcriptomic and immune profiling reveals crucial molecular pathways and hub genes associated with postoperative delirium in elderly patients DOI Creative Commons

Zhuoyue Gou,

Nan Su, Xiaochuan Li

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: May 9, 2025

Background Postoperative delirium (POD) manifests as severe mental disorientation, often experienced by elderly patients undergoing surgery, significantly hindering recovery and deteriorating the quality of life. Despite numerous clinical studies, molecular mechanisms behind POD in are still not well understood, requiring further investigation to identify potential biomarkers therapeutic targets. Methods This study amalgamates Gene Set Variation Analysis (GSVA), Weighted Co-expression Network (WGCNA), differential expression analysis, immune infiltration assessments pathways hub genes linked initiation elderly. data were sourced from GSE163943 dataset Expression Omnibus (GEO) database. A total 18,894 protein-coding extracted for analysis. Results We constructed a gene co-expression network using WGCNA performed GSVA investigate link between different types cell death. The results indicated that is positively associated with pyroptosis parthanatos, while negatively correlated oxidative stress disulfidptosis. Differential analysis revealed 145 differentially expressed (DEGs), including 83 downregulated 62 upregulated genes. functional enrichment DEGs enriched activities like neuron projection development, axonogenesis, synapse organization, KEGG pathway identifying neuroactive ligand-receptor interaction neurodegeneration pathways. Enrichment (GSEA) upregulation apoptosis downregulation interaction. Protein–protein (PPI) identified 10 genes, COL18A1, CD63, LTF. Immune occurrence strongly activation, particularly T cells macrophages. Conclusion Overall, this research primarily examines intricate interplay death processes alterations microenvironment throughout development geriatric POD, pinpointing essential provide vital theoretical support studies on POD. However, discovery only an initial one derived analyzing datasets. Upcoming ought evaluate scrutinize additional datasets conduct experiments guarantee precision widespread relevance analytical findings.

Language: Английский

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