Integrative analysis of taurine metabolism-related genes prognostic signature with immunotherapy and identification of ABCB1 and GORASP1 as key genes in nasopharyngeal carcinoma DOI Creative Commons

Feng Zhang,

Yuhang Yang,

Wenqi Luo

et al.

Amino Acids, Journal Year: 2025, Volume and Issue: 57(1)

Published: April 24, 2025

Taurine is an amino acid with several physiological functions and has been shown to be involved in the anti-tumor of human nasopharyngeal carcinoma (NPC) cells. However, role taurine metabolism-related genes (TMRGs) NPC not reported. We integrated data from Genecards, Kyoto Encyclopedia Genes Genomes (KEGG), Gene Expression Omnibus(GEO) databases identify differentially expressed associated metabolism patients. Ontology (GO) KEGG analyses were conducted investigate underlying mechanisms. Subsequently, Cox regression Least Absolute Shrinkage Selection Operator (LASSO) performed construct a prognostic signature. Survival, medication sensitivity, immunological microenvironment evaluations assess utility model. Finally, immunohistochemistry (IHC) experiments validate model's reliability. In addition, we further verified reliability our research results through molecular docking single-cell sequencing. Our model was based on three pivotal TMRGs (ABCB1, GORASP1, EZH2). Functional analysis revealed strong association between miRNAs cancer. Notably, increased risk scores correlated worsening tumor malignancy prognosis. Significant disparities immune microenvironment, checkpoints, drug sensitivity observed high- low-risk groups. The protein expression patterns selected clinical samples validated using immunohistochemistry. Molecular interaction these core taurine, which supported by sequencing showing significant variation among different cell clusters NPC. had elucidated functions, therapeutic potential, significance key related multidimensional experimental validation. This provided valuable insights potential avenues for improved management.

Language: Английский

In Silico Discovery of a Novel Natural Product Targeting PI3Kα for the Treatment of Head and Neck Squamous Cell Carcinoma DOI Open Access
Wenqing Jia,

Xian‐Chao Cheng

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3565 - 3565

Published: April 10, 2025

Head and neck squamous cell carcinoma (HNSCC) remains a major health burden, with abnormal activation of phosphatidylinositol 3-kinase alpha (PI3Kα) strongly implicated in its pathogenesis. Targeting PI3Kα represents promising therapeutic strategy. In this study, we employed structure-based virtual screening to identify natural small-molecule inhibitors PI3Kα. A total 12,800 molecules were screened, five compounds selected for further evaluation based on binding affinity interaction patterns. Pharmacokinetic properties assessed using ADMET predictions, molecular dynamics (MD) simulations conducted validate the stability. Among candidates, Apigetrin demonstrated favorable properties, high safety profile, stable within ATP-binding pocket These findings suggest that is inhibitor potential relevance HNSCC.

Language: Английский

Citations

0
Integrative analysis of taurine metabolism-related genes prognostic signature with immunotherapy and identification of ABCB1 and GORASP1 as key genes in nasopharyngeal carcinoma DOI Creative Commons

Feng Zhang,

Yuhang Yang,

Wenqi Luo

et al.

Amino Acids, Journal Year: 2025, Volume and Issue: 57(1)

Published: April 24, 2025

Taurine is an amino acid with several physiological functions and has been shown to be involved in the anti-tumor of human nasopharyngeal carcinoma (NPC) cells. However, role taurine metabolism-related genes (TMRGs) NPC not reported. We integrated data from Genecards, Kyoto Encyclopedia Genes Genomes (KEGG), Gene Expression Omnibus(GEO) databases identify differentially expressed associated metabolism patients. Ontology (GO) KEGG analyses were conducted investigate underlying mechanisms. Subsequently, Cox regression Least Absolute Shrinkage Selection Operator (LASSO) performed construct a prognostic signature. Survival, medication sensitivity, immunological microenvironment evaluations assess utility model. Finally, immunohistochemistry (IHC) experiments validate model's reliability. In addition, we further verified reliability our research results through molecular docking single-cell sequencing. Our model was based on three pivotal TMRGs (ABCB1, GORASP1, EZH2). Functional analysis revealed strong association between miRNAs cancer. Notably, increased risk scores correlated worsening tumor malignancy prognosis. Significant disparities immune microenvironment, checkpoints, drug sensitivity observed high- low-risk groups. The protein expression patterns selected clinical samples validated using immunohistochemistry. Molecular interaction these core taurine, which supported by sequencing showing significant variation among different cell clusters NPC. had elucidated functions, therapeutic potential, significance key related multidimensional experimental validation. This provided valuable insights potential avenues for improved management.

Language: Английский

Citations

0