Erbium oxide nanoparticles induce potent cell death, genomic instability and ROS-mitochondrial dysfunction-mediated apoptosis in U937 lymphoma cells DOI Creative Commons
Hanan R. H. Mohamed,

Yusuf Ahmed Elberry,

Hesham Magdy

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Erbium oxide nanoparticles (Er 2 O 3 -NPs) have attracted significant attention for their unique physicochemical properties, including high surface area, biocompatibility, and stability. However, the impact of Er -NPs on lymphoma cells (LCs) has not been explored, making this an innovative avenue exploration. Therefore, current study aimed to explore influence cell viability, genomic mitochondrial DNA integrity, reactive oxygen species (ROS) generation apoptosis induction in human U937 LCs. were characterized using X-ray diffraction (XRD) transmission electron microscopy (TEM). The effect viability integrity was estimated after 48 h WST-1 cytotoxicity alkaline Comet assays, respectively. level membrane potential also analyzed. Flow Cytometry used assess quantitative RT-PCR conducted measure apoptotic ( p53 ), anti-apoptotic Bcl2 ND3 ) gene expression. Our results demonstrated purity well distribution revealed that induce strong cells, as evidenced by a concentration-dependent reduction with IC50 value 3.20 µg/ml. Exposure LCs concentration (3.20 µg/ml) promoted excessive ROS generation, leading dramatic damage potential, marked dysregulation This cascade events triggered both necrosis -NPs-treated In conclusion, these findings highlight vitro cytotoxic against highly aggressive LCs, mediated production, which leads severe damage, profound alterations apoptotic, Future research is needed further investigate use treating optimize therapeutic efficacy.

Language: Английский

Erbium oxide nanoparticles induce potent cell death, genomic instability and ROS-mitochondrial dysfunction-mediated apoptosis in U937 lymphoma cells DOI Creative Commons
Hanan R. H. Mohamed,

Yusuf Ahmed Elberry,

Hesham Magdy

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Erbium oxide nanoparticles (Er 2 O 3 -NPs) have attracted significant attention for their unique physicochemical properties, including high surface area, biocompatibility, and stability. However, the impact of Er -NPs on lymphoma cells (LCs) has not been explored, making this an innovative avenue exploration. Therefore, current study aimed to explore influence cell viability, genomic mitochondrial DNA integrity, reactive oxygen species (ROS) generation apoptosis induction in human U937 LCs. were characterized using X-ray diffraction (XRD) transmission electron microscopy (TEM). The effect viability integrity was estimated after 48 h WST-1 cytotoxicity alkaline Comet assays, respectively. level membrane potential also analyzed. Flow Cytometry used assess quantitative RT-PCR conducted measure apoptotic ( p53 ), anti-apoptotic Bcl2 ND3 ) gene expression. Our results demonstrated purity well distribution revealed that induce strong cells, as evidenced by a concentration-dependent reduction with IC50 value 3.20 µg/ml. Exposure LCs concentration (3.20 µg/ml) promoted excessive ROS generation, leading dramatic damage potential, marked dysregulation This cascade events triggered both necrosis -NPs-treated In conclusion, these findings highlight vitro cytotoxic against highly aggressive LCs, mediated production, which leads severe damage, profound alterations apoptotic, Future research is needed further investigate use treating optimize therapeutic efficacy.

Language: Английский

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