Lamin A/C regulates cerebellar granule cell maturation DOI Creative Commons

Laura Vilardo,

Ingrid Cifola, Marta Nardella

et al.

Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)

Published: April 5, 2025

Abstract Lamin A/C is a nuclear type V intermediate filament protein part of the meshwork structure underlying inner membrane (nuclear lamina), which plays numerous roles, including maintenance shape, heterochromatin organization, and transcriptional regulation. Our group has demonstrated role in different pathophysiological conditions. Here, we investigated for first time how affects neuronal maturation rat cerebellar granule cells (GCs). Primary GCs where silenced Lmna gene constituted our key model; this provided rather homogeneous cellular system showing population vitro. We then validated findings another vivo murine model with knock-out an vitro human silencing LMNA gene. observed across three models that down-regulation neurons by protecting from glutamate-evoked excitotoxicity correlates inhibition calcium influxes pro-inflammatory cytokine pathways. Consistent previous group, study corroborates neural development opens new significant implications better comprehension mechanisms involved neurodegenerative diseases, changes envelope are linked to neuroinflammatory processes damage.

Language: Английский

Lamin A/C regulates cerebellar granule cell maturation DOI Creative Commons

Laura Vilardo,

Ingrid Cifola, Marta Nardella

et al.

Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)

Published: April 5, 2025

Abstract Lamin A/C is a nuclear type V intermediate filament protein part of the meshwork structure underlying inner membrane (nuclear lamina), which plays numerous roles, including maintenance shape, heterochromatin organization, and transcriptional regulation. Our group has demonstrated role in different pathophysiological conditions. Here, we investigated for first time how affects neuronal maturation rat cerebellar granule cells (GCs). Primary GCs where silenced Lmna gene constituted our key model; this provided rather homogeneous cellular system showing population vitro. We then validated findings another vivo murine model with knock-out an vitro human silencing LMNA gene. observed across three models that down-regulation neurons by protecting from glutamate-evoked excitotoxicity correlates inhibition calcium influxes pro-inflammatory cytokine pathways. Consistent previous group, study corroborates neural development opens new significant implications better comprehension mechanisms involved neurodegenerative diseases, changes envelope are linked to neuroinflammatory processes damage.

Language: Английский

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