Integrated multi-omics analysis reveals the functional and prognostic significance of lactylation-related gene PRDX1 in breast cancer DOI Creative Commons

Qinqing Wu,

Heng Cao,

J. Jin

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: April 4, 2025

Background Breast cancer (BRCA) is a significant threat to women’s health worldwide, and its progression closely associated with the tumor microenvironment gene regulation. Lactylation modification, as key epigenetic mechanism in biology, has not yet been fully elucidated context of BRCA. This study examines regulatory mechanisms lactylation-related genes (LRGs), specifically PRDX1, their prognostic significance Methods We integrated data from multiple databases, including Genome-Wide Association Study (GWAS) summary statistics, single-cell RNA sequencing, spatial transcriptomics, bulk sequencing The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases. Using Summary-based Mendelian Randomization (SMR) analysis, we identified LRGs BRCA comprehensively analysed expression patterns cell-cell communication networks, heterogeneity. Furthermore, constructed validated model based on profile PRDX1-positive monocytes, evaluating it through Cox regression LASSO analyses. Results PRDX1 was LRG significantly risk (p_SMR = 0.0026). Single-cell analysis revealed upregulation enhanced between monocytes fibroblasts. Spatial transcriptomics uncovered heterogeneous nest regions, highlighting interaction demonstrated high accuracy predicting patient survival both training validation cohorts. High-risk patients exhibited immune-suppressive characteristics, reduced immune cell infiltration checkpoint expression. Conclusion reveals role progression, mainly regulation escape mechanisms. prediction shows robust potential, future research should focus integrating other biomarkers enhance precision personalised medicine.

Language: Английский

Integrated multi-omics analysis reveals the functional and prognostic significance of lactylation-related gene PRDX1 in breast cancer DOI Creative Commons

Qinqing Wu,

Heng Cao,

J. Jin

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: April 4, 2025

Background Breast cancer (BRCA) is a significant threat to women’s health worldwide, and its progression closely associated with the tumor microenvironment gene regulation. Lactylation modification, as key epigenetic mechanism in biology, has not yet been fully elucidated context of BRCA. This study examines regulatory mechanisms lactylation-related genes (LRGs), specifically PRDX1, their prognostic significance Methods We integrated data from multiple databases, including Genome-Wide Association Study (GWAS) summary statistics, single-cell RNA sequencing, spatial transcriptomics, bulk sequencing The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases. Using Summary-based Mendelian Randomization (SMR) analysis, we identified LRGs BRCA comprehensively analysed expression patterns cell-cell communication networks, heterogeneity. Furthermore, constructed validated model based on profile PRDX1-positive monocytes, evaluating it through Cox regression LASSO analyses. Results PRDX1 was LRG significantly risk (p_SMR = 0.0026). Single-cell analysis revealed upregulation enhanced between monocytes fibroblasts. Spatial transcriptomics uncovered heterogeneous nest regions, highlighting interaction demonstrated high accuracy predicting patient survival both training validation cohorts. High-risk patients exhibited immune-suppressive characteristics, reduced immune cell infiltration checkpoint expression. Conclusion reveals role progression, mainly regulation escape mechanisms. prediction shows robust potential, future research should focus integrating other biomarkers enhance precision personalised medicine.

Language: Английский

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