Whole Genome and Comparative Genomic Analysis of Bacillus sp. KNSH11 Isolated from the Gut of Litopenaeus vannamei Reveals Its Probiotic Potential DOI

Waraphorn Sihamok,

Sk Injamamul Islam, Lưu Tăng Phúc Khang

et al.

Published: May 13, 2025

Abstract In sustainable aquaculture, probiotics offer a promising alternative to antibiotics for improving shrimp health. Bacillus sp. KNSH11, isolated from the intestine of whiteleg (Litopenaeus vannamei), was characterized evaluate its probiotic potential. The strain, Gram-positive, rod-shaped bacillus, exhibited exceptional spore formation efficiency (> 99%), ensuring resilience in challenging environments. Functional assays demonstrated that KNSH11 maintained high viability at pH 2–4, presence bile salts, temperature up 95°C, and under lysozyme exposure, indicating tolerance gastrointestinal processing stresses. Metabolic profiling indicated significant lactic acid production with minimal acetate propionate, distinguishing it conventional bacteria. also displayed strong antioxidative activities moderate antibiofilm effects against pathogens. Antibiotic susceptibility testing revealed sensitivity amoxicillin (30 µg/disc), chloramphenicol kanamycin µg/disc) tetracyclines but resistance ampicillin (10 penicillin µg/disc). Whole genome sequencing (WGS) confirmed absence virulence factors identified mobile genetic elements, CRISPR/Cas system, gene clusters potentially encoding bacteriocins. Collectively, these findings suggest is safe, eco-friendly potential enhance health advance aquaculture.

Language: Английский

Whole Genome and Comparative Genomic Analysis of Bacillus sp. KNSH11 Isolated from the Gut of Litopenaeus vannamei Reveals Its Probiotic Potential DOI

Waraphorn Sihamok,

Sk Injamamul Islam, Lưu Tăng Phúc Khang

et al.

Published: May 13, 2025

Abstract In sustainable aquaculture, probiotics offer a promising alternative to antibiotics for improving shrimp health. Bacillus sp. KNSH11, isolated from the intestine of whiteleg (Litopenaeus vannamei), was characterized evaluate its probiotic potential. The strain, Gram-positive, rod-shaped bacillus, exhibited exceptional spore formation efficiency (> 99%), ensuring resilience in challenging environments. Functional assays demonstrated that KNSH11 maintained high viability at pH 2–4, presence bile salts, temperature up 95°C, and under lysozyme exposure, indicating tolerance gastrointestinal processing stresses. Metabolic profiling indicated significant lactic acid production with minimal acetate propionate, distinguishing it conventional bacteria. also displayed strong antioxidative activities moderate antibiofilm effects against pathogens. Antibiotic susceptibility testing revealed sensitivity amoxicillin (30 µg/disc), chloramphenicol kanamycin µg/disc) tetracyclines but resistance ampicillin (10 penicillin µg/disc). Whole genome sequencing (WGS) confirmed absence virulence factors identified mobile genetic elements, CRISPR/Cas system, gene clusters potentially encoding bacteriocins. Collectively, these findings suggest is safe, eco-friendly potential enhance health advance aquaculture.

Language: Английский

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