Uncovering a novel biosynthetic gene cluster for sordarin through genome mining in the fungus Talaromyces adpressus DOI Creative Commons
Qianqian Xu,

Xiaomeng Ren,

Linzhen Hu

et al.

Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 17, 2025

Abstract To explore the chemical and biological diversities of diterpenoids from fungus Talaromyces adpressus , a previously unknown biosynthetic gene cluster (BGC, tdn ) for sordarin (a well-known fungal antibiotics) was discovered by leveraging genome mining method. Heterologous expressions key genes in Aspergillus oryzae led to determination one new diterpenoid, cycloaraneosene-9-ol-8-one ( 4 ), three known diterpenoids, cycloaraneosene 1 cycloaraneosene-9-ol 2 cycloaraneosene-8,9-diol 3 ). The structures – elucidated well via detailed analysis 1D 2D NMR, GCMS, HRESIMS, IR data, comparison with reported data. Structurally, compounds were belonging fusicoccane classical tricyclic 5/8/5 ring system, which are participated biosynthesis sordarin. Compound maybe precursor Baeyer–Villiger like reaction C8–C9 bond cleavage pathway Moreover, all isolates evaluated their bioactivities, exhibited inhibitory activities against human cancer cell lines IC 50 values ranging 7.8 32.4 µ M. promote apoptosis HCT-116 HepG2 cells, suppress migration cells. As well, also decrease expression proliferation related molecules BCL-2 cyclin D1, while increase BAX. Targets predication molecular docking indicate that compound exhibits stronger affinity DBL, suggesting its excellent binding potential. This finding will be enriched bioactivities most importantly, provide strategies synthetic research sordarins. Graphical

Language: Английский

Iminium catalysis meets Diels–Alderase DOI
Naoki Kato, Shingo Nagano

Nature Catalysis, Journal Year: 2025, Volume and Issue: 8(3), P. 202 - 203

Published: March 26, 2025

Citations

0
Uncovering a novel biosynthetic gene cluster for sordarin through genome mining in the fungus Talaromyces adpressus DOI Creative Commons
Qianqian Xu,

Xiaomeng Ren,

Linzhen Hu

et al.

Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 17, 2025

Abstract To explore the chemical and biological diversities of diterpenoids from fungus Talaromyces adpressus , a previously unknown biosynthetic gene cluster (BGC, tdn ) for sordarin (a well-known fungal antibiotics) was discovered by leveraging genome mining method. Heterologous expressions key genes in Aspergillus oryzae led to determination one new diterpenoid, cycloaraneosene-9-ol-8-one ( 4 ), three known diterpenoids, cycloaraneosene 1 cycloaraneosene-9-ol 2 cycloaraneosene-8,9-diol 3 ). The structures – elucidated well via detailed analysis 1D 2D NMR, GCMS, HRESIMS, IR data, comparison with reported data. Structurally, compounds were belonging fusicoccane classical tricyclic 5/8/5 ring system, which are participated biosynthesis sordarin. Compound maybe precursor Baeyer–Villiger like reaction C8–C9 bond cleavage pathway Moreover, all isolates evaluated their bioactivities, exhibited inhibitory activities against human cancer cell lines IC 50 values ranging 7.8 32.4 µ M. promote apoptosis HCT-116 HepG2 cells, suppress migration cells. As well, also decrease expression proliferation related molecules BCL-2 cyclin D1, while increase BAX. Targets predication molecular docking indicate that compound exhibits stronger affinity DBL, suggesting its excellent binding potential. This finding will be enriched bioactivities most importantly, provide strategies synthetic research sordarins. Graphical

Language: Английский

Citations

0