Engineering CAR‐T Therapeutics for Enhanced Solid Tumor Targeting DOI Creative Commons
Danqing Zhu, Wonjoon Kim, Hyunjin Lee

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Cancer immunotherapy, specifically Chimeric Antigen Receptor (CAR)‐T cell therapy, represents a significant breakthrough in treating cancers. Despite its success hematological cancers, CAR‐T exhibits limited efficacy solid tumors, which account for more than 90% of all Solid tumors commonly present unique challenges, including antigen heterogeneity and complex tumor microenvironment (TME). To address these, efforts are being made through improvements CAR design the development advanced validation platforms. While is limited, some types, such as neuroblastoma gastrointestinal have shown responsiveness to therapy recent clinical trials. In this review, it first examined both experimental computational strategies, protein engineering coupled with machine learning, developed enhance T specificity. The challenges methods associated delivery vivo reprogramming discussed. It also explored advancements engineered organoid systems, emerging high‐fidelity vitro models that closely mimic human TME serve platform discovery. Collectively, these innovative strategies offer potential revolutionize next generation ultimately paving way effective treatments tumors.

Language: Английский

Organoids DOI Creative Commons
Zixuan Zhao, Xinyi Chen, Anna M. Dowbaj

et al.

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: Dec. 1, 2022

Organoids have attracted increasing attention because they are simple tissue-engineered cell-based in vitro models that recapitulate many aspects of the complex structure and function corresponding vivo tissue. They can be dissected interrogated for fundamental mechanistic studies on development, regeneration, repair human tissues. also used diagnostics, disease modeling, drug discovery, personalized medicine. derived from either pluripotent or tissue-resident stem (embryonic adult) progenitor differentiated cells healthy diseased tissues, such as tumors. To date, numerous organoid engineering strategies support culture growth, proliferation, differentiation maturation been reported. This Primer serves to highlight rationale underlying selection development these materials methods control cellular/tissue niche; therefore, engineered organoid. We discuss key considerations generating robust organoids, those related cell isolation seeding, matrix soluble factor selection, physical cues integration. The general standards data quality, reproducibility deposition within community is outlined. Lastly, we conclude by elaborating limitations organoids different applications, priorities coming years.

Language: Английский

Citations

479
Human organoids: New strategies and methods for analyzing human development and disease DOI Creative Commons
Nina S. Corsini, Juergen A. Knoblich

Cell, Journal Year: 2022, Volume and Issue: 185(15), P. 2756 - 2769

Published: July 1, 2022

For decades, insight into fundamental principles of human biology and disease has been obtained primarily by experiments in animal models. While this allowed researchers to understand many biological processes great detail, some developmental mechanisms have proven difficult study due inherent species differences. The advent organoid technology more than 10 years ago established laboratory-grown organ tissues as an additional model system recapitulate human-specific aspects biology. use 3D organoids, well other advances single-cell technologies, revealed unprecedented insights mechanisms, especially those that distinguish humans from species. This review highlights novel with a focus on how generated better understanding development disease.

Language: Английский

Citations

105
AI-enabled organoids: Construction, analysis, and application DOI Creative Commons
Long Bai,

Yan Wu,

Guangfeng Li

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 31, P. 525 - 548

Published: Sept. 16, 2023

Organoids, miniature and simplified

Language: Английский

Citations

79
Patient-derived organoids in translational oncology and drug screening DOI Creative Commons
Ruixin Yang, Yingyan Yu

Cancer Letters, Journal Year: 2023, Volume and Issue: 562, P. 216180 - 216180

Published: April 13, 2023

Patient-derived organoids (PDO) are a new biomedical research model that can reconstruct phenotypic and genetic characteristics of the original tissue useful for on pathogenesis drug screening. To introduce progression in this field, we review key factors constructing derived from epithelial tissues cancers, covering culture medium matrix, morphological characteristics, profiles, high-throughput screening, application potential. We also discuss co-culture system cancer with tumor microenvironment (TME) associated cells. The is widely used evaluating crosstalk cells TME components, such as fibroblasts, endothelial cells, immune microorganisms. article provides prospective standardized cultivation mode, automatic evaluation, sensitivity screening using methods.

Language: Английский

Citations

58
Recent advances in defined hydrogels in organoid research DOI Creative Commons
Zhongqiao Gan,

Xinyuan Qin,

Haitao Liu

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 28, P. 386 - 401

Published: June 16, 2023

Organoids are in vitro model systems that mimic the complexity of organs with multicellular structures and functions, which provide great potential for biomedical tissue engineering. However, their current formation heavily relies on using complex animal-derived extracellular matrices (ECM), such as Matrigel. These often poorly defined chemical components exhibit limited tunability reproducibility. Recently, biochemical biophysical properties hydrogels can be precisely tuned, offering broader opportunities to support development maturation organoids. In this review, fundamental ECM vivo critical strategies design organoid culture summarized. Two typically derived from natural synthetic polymers applicability improve organoids presented. The representative applications incorporating into highlighted. Finally, some challenges future perspectives also discussed developing advanced technologies toward supporting research.

Language: Английский

Citations

55
Replacement, Reduction, and Refinement of Animal Experiments in Anticancer Drug Development: The Contribution of 3D In Vitro Cancer Models in the Drug Efficacy Assessment DOI Creative Commons
Elena M. Tosca, Davide Ronchi,

Daniele Facciolo

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(4), P. 1058 - 1058

Published: March 30, 2023

In the last decades three-dimensional (3D) in vitro cancer models have been proposed as a bridge between bidimensional (2D) cell cultures and vivo animal models, gold standards preclinical assessment of anticancer drug efficacy. 3D can be generated through multitude techniques, from both immortalized lines primary patient-derived tumor tissue. Among them, spheroids organoids represent most versatile promising they faithfully recapitulate complexity heterogeneity human cancers. Although their recent applications include screening programs personalized medicine, not yet established tools for studying efficacy supporting preclinical-to-clinical translation, which remains mainly based on experimentation. this review, we describe state-of-the-art evaluation agents, focusing potential contribution to replace, reduce refine experimentations, highlighting strength weakness, discussing possible perspectives overcome current challenges.

Language: Английский

Citations

51
Towards a better understanding of diabetes mellitus using organoid models DOI Open Access
Belin Selcen Beydag-Tasöz, Siham Yennek, Anne Grapin‐Botton

et al.

Nature Reviews Endocrinology, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 20, 2023

Language: Английский

Citations

49
Design and engineering of organ-on-a-chip DOI Open Access
Su Jin Cho, Sumi Lee, Song Ih Ahn

et al.

Biomedical Engineering Letters, Journal Year: 2023, Volume and Issue: 13(2), P. 97 - 109

Published: Jan. 2, 2023

Language: Английский

Citations

44
Research Progress in Hydrogels for Cartilage Organoids DOI

Xiaolong Li,

Shihao Sheng, Guangfeng Li

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(22)

Published: May 21, 2024

The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development

Language: Английский

Citations

30
Biomimetic Hydrogels – Tools for Regenerative Medicine, Oncology, and Understanding Medical Gas Plasma Therapy DOI Creative Commons

Alice Martinet,

Lea Miebach, Klaus‐Dieter Weltmann

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Abstract Biomimetic hydrogels enable biochemical, cell biology, and tissue‐like studies in the third dimension. Smart are also frequently used tissue engineering as drug carriers for intra‐ or extracutaneous regenerative medicine. They have been studied bio‐sensor development, 3D culture, organoid growth optimization. Yet, many hydrogel types, adjuvant components, cross‐linking methods emerged over decades, diversifying complexifying such studies. Here, an evaluative overview is provided, mapping potential applications to corresponding tuning. Strikingly, ideal studying locoregional therapy modalities, cold medical gas plasma technology. These partially ionized gases produce various reactive oxygen species (ROS) types along with other physico‐chemical components ions electric fields, spatio‐temporal effects of these delivered diseased tissues remain largely elusive date. Hence, this work outlines promising biomedical research general science particular underlines great smart scaffolds current future therapy.

Language: Английский

Citations

2