Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of Fast-Growing Bacteria DOI Creative Commons
Leticia Larotonda,

E. Ojeda,

Matteo Bordignon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract How gene order along chromosomes affects cellular homeostasis and genome evolution remains poorly understood. Bacterial are organized the replication origin ( oriC )–terminus ter ) axis. The spatial arrengement of genes within this axis may influence physiology, evolution, transcriptional regulation. catalytic core sole bacterial RNA polymerase (RNAP) is encoded by rpoB rpoC universally conserved rplKAJL-rpoBC locus. In fast growing bacteria locus located near potentially linking organization to physiology. Here, we investigated functional relevance chromosomal positioning relocating it Vibrio cholerae genome. While viable, strains harboring far from exhibited nutrient-dependent fitness defects. These phenotypes correlated with reduced copy number RNAP abundance, without changes in its subcellular distribution. Introducing a second rpIKAJL-rpoBC at distal genomic location abolished phenotypes, demonstrating that replication-associated dosage effects were main mechanism behind observed physiological changes. Further uncoupling revealed costs specifically linked relocation. Our results reveal selective pressures act maintain RNAP-encoding optimize effects, ensuring efficient production during exponential growth. We discuss relationship between ecological strategies work underscores as an underestimated but critical factor shaping physiology evolution. Significance have highly plastic content importance their started be appreciated driver Growing evidence suggests genomes plays role expression, dynamics, fitness. study, demonstrate , encoding (RNAP), essential for optimal By show position directly impacts fitness, effects. findings suggest ensure output More broadly, our study highlights underappreciated beyond known roles operon structure Given fundamental RNAP, implications understanding strategies, adaptation, synthetic design. integrating positional genomics studies, provide theoretical experimental framework investigating how architecture shapes function.

Language: Английский

Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of Fast-Growing Bacteria DOI Creative Commons
Leticia Larotonda,

E. Ojeda,

Matteo Bordignon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract How gene order along chromosomes affects cellular homeostasis and genome evolution remains poorly understood. Bacterial are organized the replication origin ( oriC )–terminus ter ) axis. The spatial arrengement of genes within this axis may influence physiology, evolution, transcriptional regulation. catalytic core sole bacterial RNA polymerase (RNAP) is encoded by rpoB rpoC universally conserved rplKAJL-rpoBC locus. In fast growing bacteria locus located near potentially linking organization to physiology. Here, we investigated functional relevance chromosomal positioning relocating it Vibrio cholerae genome. While viable, strains harboring far from exhibited nutrient-dependent fitness defects. These phenotypes correlated with reduced copy number RNAP abundance, without changes in its subcellular distribution. Introducing a second rpIKAJL-rpoBC at distal genomic location abolished phenotypes, demonstrating that replication-associated dosage effects were main mechanism behind observed physiological changes. Further uncoupling revealed costs specifically linked relocation. Our results reveal selective pressures act maintain RNAP-encoding optimize effects, ensuring efficient production during exponential growth. We discuss relationship between ecological strategies work underscores as an underestimated but critical factor shaping physiology evolution. Significance have highly plastic content importance their started be appreciated driver Growing evidence suggests genomes plays role expression, dynamics, fitness. study, demonstrate , encoding (RNAP), essential for optimal By show position directly impacts fitness, effects. findings suggest ensure output More broadly, our study highlights underappreciated beyond known roles operon structure Given fundamental RNAP, implications understanding strategies, adaptation, synthetic design. integrating positional genomics studies, provide theoretical experimental framework investigating how architecture shapes function.

Language: Английский

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