Single-Nucleus Transcriptomics Reveals Prenatal and Postnatal Pb Exposure-Induced Cell-Specific Neurotoxicity and Dysregulated Microglia-Neuron Communication in Mice Brain DOI

Xuting Liu,

Chunfeng Huang,

Mingyue Wang

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Lead (Pb) is an environmental pollutant that has lasting effects on neurodevelopment. Children exhibit heightened sensitivity to Pb exposure compared adults, and prenatal can harm the developing fetal nervous system. However, specific regulatory of across various developmental stages are not well understood. This study employed single-nucleus RNA sequencing (snRNA-seq) analyze mice brains at different ages (2 8 weeks) following postnatal exposure. Blood lead level in exposed comparable those detected human samples, implying its implication. A total 43,303 brain cells were sequenced for cell-specific analysis. was found elevate proportion immature neurons 2 week-old perturb neurodevelopment- neural structure-related pathways within neurons. In mice, primarily influenced implicated synaptic transmission, signal transduction, learning memory both glial cells. The communication involving neurotransmitters glutamate γ-aminobutyric acid (GABA), along with their receptors, disrupted between neuron microglia. Through application snRNA-seq, this demonstrated Pb-induced neurotoxicity characterized by cellular heterogeneity disruption neurotransmitter-related microglia could be a critical factor neurotoxicity.

Language: Английский

Single-Nucleus Transcriptomics Reveals Prenatal and Postnatal Pb Exposure-Induced Cell-Specific Neurotoxicity and Dysregulated Microglia-Neuron Communication in Mice Brain DOI

Xuting Liu,

Chunfeng Huang,

Mingyue Wang

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Lead (Pb) is an environmental pollutant that has lasting effects on neurodevelopment. Children exhibit heightened sensitivity to Pb exposure compared adults, and prenatal can harm the developing fetal nervous system. However, specific regulatory of across various developmental stages are not well understood. This study employed single-nucleus RNA sequencing (snRNA-seq) analyze mice brains at different ages (2 8 weeks) following postnatal exposure. Blood lead level in exposed comparable those detected human samples, implying its implication. A total 43,303 brain cells were sequenced for cell-specific analysis. was found elevate proportion immature neurons 2 week-old perturb neurodevelopment- neural structure-related pathways within neurons. In mice, primarily influenced implicated synaptic transmission, signal transduction, learning memory both glial cells. The communication involving neurotransmitters glutamate γ-aminobutyric acid (GABA), along with their receptors, disrupted between neuron microglia. Through application snRNA-seq, this demonstrated Pb-induced neurotoxicity characterized by cellular heterogeneity disruption neurotransmitter-related microglia could be a critical factor neurotoxicity.

Language: Английский

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