Tuning the Morphological Properties of Granular Hydrogels to Control Lymphatic Capillary Formation DOI Creative Commons
Daniel Montes, Sanjoy Saha,

Angela Taglione

et al.

Advanced Materials Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Granular hydrogels show great promise in biomedical applications by mimicking the extracellular matrix and fostering a supportive microenvironment for tissue regeneration. This study investigates how tuning granular hydrogel properties influences lymphatic tube formation. Microgels were fabricated using norbornene‐modified hyaluronic acid (NorHA) via pipetting or vortexing 90 s (V90s) 180 (V180s), then assembled into under loose tight packing conditions. These conditions produced gels with varied pore morphologies bulk rheological properties. Lymphatic capillary formation occurred only tightly packed gels, where mechanical converged, highlighting importance of gel morphology over stiffness. V180s samples showed earlier vessel as seen gene protein expression, while pipetted exhibited greater connectivity, forming larger clusters fewer small satellite structures. The also supported lower‐curvature, more linear networks that bridged multiple droplets, likely due to reduced entrapment large voids compared vortexed gels. findings suggest is governed not stiffness but size topology (periodicity). Understanding optimizing these morphological parameters can inform future strategies engineering regenerative medicine.

Language: Английский

Tissue‐Engineered Therapeutics for Lymphatic Regeneration: Solutions for Myocardial Infarction and Secondary Lymphedema DOI Creative Commons
Alvis Chiu, Joseph M. Rutkowski,

Qixu Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract The lymphatic system, which regulates inflammation and fluid homeostasis, is damaged in various diseases including myocardial infarction (MI) breast‐cancer‐related lymphedema (BCRL). Mounting evidence suggests that restoring tissue drainage clearing excess immune cells by regenerating vessels can aid cardiac repair amelioration. Current treatments primarily address symptoms rather than underlying causes due to a lack of regenerative therapies, highlighting the importance system as promising novel therapeutic target. Here cutting‐edge research on engineered tissues, growth factor cell‐based approaches designed enhance lymphangiogenesis restore function explored. Special focus placed how therapies with potential for immediate reconstruction, originally treating BCRL, be applied MI augment reduce heart failure risk. integration these significantly improve patient outcomes promoting repair, preventing pathological remodeling, offering new avenues managing lymphatic‐associated diseases.

Language: Английский

Citations

0
Tuning the Morphological Properties of Granular Hydrogels to Control Lymphatic Capillary Formation DOI Creative Commons
Daniel Montes, Sanjoy Saha,

Angela Taglione

et al.

Advanced Materials Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Granular hydrogels show great promise in biomedical applications by mimicking the extracellular matrix and fostering a supportive microenvironment for tissue regeneration. This study investigates how tuning granular hydrogel properties influences lymphatic tube formation. Microgels were fabricated using norbornene‐modified hyaluronic acid (NorHA) via pipetting or vortexing 90 s (V90s) 180 (V180s), then assembled into under loose tight packing conditions. These conditions produced gels with varied pore morphologies bulk rheological properties. Lymphatic capillary formation occurred only tightly packed gels, where mechanical converged, highlighting importance of gel morphology over stiffness. V180s samples showed earlier vessel as seen gene protein expression, while pipetted exhibited greater connectivity, forming larger clusters fewer small satellite structures. The also supported lower‐curvature, more linear networks that bridged multiple droplets, likely due to reduced entrapment large voids compared vortexed gels. findings suggest is governed not stiffness but size topology (periodicity). Understanding optimizing these morphological parameters can inform future strategies engineering regenerative medicine.

Language: Английский

Citations

0