Loureirin B Accelerates Diabetic Wound Healing by Promoting TGFβ/Smad‐Dependent Macrophage M2 Polarization: A Concerted Analytical Approach Through Single‐Cell RNA Sequencing and Experimental Verification DOI
Weijing Fan, Yin Qu, Xin Yuan

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

ABSTRACT Diabetic wound (DW) represent a significant clinical challenge and often fail to heal effectively. Loureirin B (LB), flavonoid extracted from dragon's blood, has shown potential by influencing macrophage polarization promoting healing. However, its mechanisms efficacy in DW remain be explored. This study employed single‐cell RNA sequencing analyze the classification of cells diabetic foot ulcers identify related influenced macrophages. Molecular docking was used predict interactions LB with key proteins TGFβ/Smad signaling pathway. The effects on healing were further validated through vitro vivo experiments using model. Single‐cell analysis identified specific subtypes involved process highlighted role suggested action within In studies showed that under high glucose conditions, promoted pro‐inflammatory M1 healing‐promoting M2 ECM production fibroblasts activating TGF‐β/Smad signaling. vivo, treatment enhanced rates mice fibroblast synthesis regulates promote These findings suggest could therapeutic agent for improving healing, emphasizing need explore human subjects.

Language: Английский

Red-light LED therapy promotes wound regeneration by upregulating COL1A1, COL2A1, VEGF and reducing IL-1β for anti-inflammation DOI
Sree Samanvitha Kuppa, Ju Yeon Kang,

Ja-Yeon Kim

et al.

Lasers in Medical Science, Journal Year: 2025, Volume and Issue: 40(1)

Published: April 3, 2025

Language: Английский

Citations

0
Low‐Irradiance Antimicrobial Blue Light‐Bathing Therapy for Wound Infection Control DOI Creative Commons
Jie Hui,

Wonjoon Moon,

Pu‐Ting Dong

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract The prevalence of antibiotic resistance and tolerance in wound infection management poses a serious growing health threat, necessitating the exploration alternative approaches. Antimicrobial blue light therapy offers an appealing, non‐pharmacological solution. However, its practical application has been hindered by requirement for high irradiance levels (50–200 mW/cm 2 ), which particularly raises safety concerns. Here, light‐bathing strategy is introduced that employs prolonged, continuous exposure to at range lower more than order magnitude (5 ). This method consistently applies bacteriostatic pressure, keeping bioburden low, all while minimizing photothermal risks. Leveraging tailor‐made, wearable light‐emitting patches, preclinical trials on rat models are conducted, demonstrating efficacy suppressing infections induced methicillin‐resistant Staphylococcus aureus ( S. ) multidrug‐resistant Pseudomonas aeruginosa P. results pave new way care.

Language: Английский

Citations

0
Beyond ultraviolet radiation: Immune system modulation through skin exposure to visible light and infrared radiation DOI
Nicolás Garimano,

Taís Aguayo Frías,

Daniel H. González Maglio

et al.

Photochemistry and Photobiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

Abstract Sunlight profoundly affects skin health when it is exposed. After acute exposure, a robust inflammatory response initiated locally. Moreover, chronic exposures lead to carcinogenesis and photoaging. Local systemic immunosuppression also triggered after irradiation, affecting adaptive immune responses. These effects are mainly produced by the ultraviolet radiation contained in sunlight were extensively described reviewed. However, using UV filters during sunbathing outdoor activities may allow visible light (VL) wavelengths infrared (IRR) reach cells. Additionally, employment of therapeutic VL IR‐emitting lasers LED devices increasing for various conditions. This literature review aims present current knowledge on IRR modulating system. modulations impact healthy can modify responses diverse stimuli cell types. According wavelength dose, increase production reactive oxygen species promote faster wound healing. they modulate mediators, such as several cytokines prostaglandins. exposure affect The study immunity would new uses phototherapy establish need strategies photoprotection.

Language: Английский

Citations

0
Loureirin B Accelerates Diabetic Wound Healing by Promoting TGFβ/Smad‐Dependent Macrophage M2 Polarization: A Concerted Analytical Approach Through Single‐Cell RNA Sequencing and Experimental Verification DOI
Weijing Fan, Yin Qu, Xin Yuan

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

ABSTRACT Diabetic wound (DW) represent a significant clinical challenge and often fail to heal effectively. Loureirin B (LB), flavonoid extracted from dragon's blood, has shown potential by influencing macrophage polarization promoting healing. However, its mechanisms efficacy in DW remain be explored. This study employed single‐cell RNA sequencing analyze the classification of cells diabetic foot ulcers identify related influenced macrophages. Molecular docking was used predict interactions LB with key proteins TGFβ/Smad signaling pathway. The effects on healing were further validated through vitro vivo experiments using model. Single‐cell analysis identified specific subtypes involved process highlighted role suggested action within In studies showed that under high glucose conditions, promoted pro‐inflammatory M1 healing‐promoting M2 ECM production fibroblasts activating TGF‐β/Smad signaling. vivo, treatment enhanced rates mice fibroblast synthesis regulates promote These findings suggest could therapeutic agent for improving healing, emphasizing need explore human subjects.

Language: Английский

Citations

1