RNA-guided nucleases enable a gene drive of insertion sequences in plasmids DOI Creative Commons
Kepler Mears, Fernando W. Rossine, Natalia Quinones‐Olvera

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Abstract Mobile genetic elements (MGEs) and the interactions between them are a major source of evolutionary innovation. Insertion sequences, simplest MGEs usually encoding only necessary genes for transposition maintenance, widespread in bacterial genomes, particularly common plasmids. Plasmids, self-replicating extrachromosomal DNA elements, often exist multiple copies imparting stochastic barrier to fixation an insertion sequence by limiting proportion plasmid population harboring IS. In this work we demonstrate that overcome this, IS200/605 family sequences utilizes programmable RNA guided nucleases as gene drive spread IS through population. TnpB, likely ancestor Cas12, records specific site its guide prevent loss during transposition. When introduced TnpB will be reprogrammed target cleave IS-plasmids, resulting biased replication IS+ Furthermore, activity is critical invade high copy populations. Because can mobilized microbes on other mobile advantage fixing plasmids may help explain prevalence across tree life. More generally, unique pressures arising from movement contexts with different multiplicities shapes evolution strategies MGE spread.

Language: Английский

RNA-guided nucleases enable a gene drive of insertion sequences in plasmids DOI Creative Commons
Kepler Mears, Fernando W. Rossine, Natalia Quinones‐Olvera

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Abstract Mobile genetic elements (MGEs) and the interactions between them are a major source of evolutionary innovation. Insertion sequences, simplest MGEs usually encoding only necessary genes for transposition maintenance, widespread in bacterial genomes, particularly common plasmids. Plasmids, self-replicating extrachromosomal DNA elements, often exist multiple copies imparting stochastic barrier to fixation an insertion sequence by limiting proportion plasmid population harboring IS. In this work we demonstrate that overcome this, IS200/605 family sequences utilizes programmable RNA guided nucleases as gene drive spread IS through population. TnpB, likely ancestor Cas12, records specific site its guide prevent loss during transposition. When introduced TnpB will be reprogrammed target cleave IS-plasmids, resulting biased replication IS+ Furthermore, activity is critical invade high copy populations. Because can mobilized microbes on other mobile advantage fixing plasmids may help explain prevalence across tree life. More generally, unique pressures arising from movement contexts with different multiplicities shapes evolution strategies MGE spread.

Language: Английский

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