Nanotechnology-driven gene silencing: advancements in SIGS–dsRNA technology for sustainable disease management

Chemical and Biological Technologies in Agriculture, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 4, 2025

Among the fungal diseases, Fusarium head blight (FHB), caused by graminearum, is one of most destructive disease affecting wheat. This pathogen poses significant threats to global wheat production, leading substantial yield losses and contaminating grains with harmful mycotoxins. The chemical control FHB has become increasingly challenging due rise resistance, environmental concerns, effects climate change. review introduces a novel approach management through spray-induced gene silencing (SIGS), cutting-edge technology that uses double-stranded RNA (dsRNA) silence critical genes in both fungus host plant. reduces virulence enhances plant resilience. A key innovation integration nanotechnology improve delivery dsRNA, addressing challenges related stability, cellular uptake, targeting efficiency field conditions. Nanocarriers have revolutionized dsRNA improving its encapsulation efficiency, precision, compared traditional methods. Advances cost-effective particularly microbial expression systems, enable scalable sustainable implementation this technology. emphasizes potential nanocarrier systems precision agriculture highlights their role replacing treatments interference (RNAi)-based solutions. RNAi-based approaches not only reduce reliance on synthetic chemicals, but also promote sustainability fungicide resistance. However, remain large-scale application, cost-effectiveness, regulatory approval processes. Overcoming these hurdles will be crucial unlocking full In conclusion, combination SIGS-based offers groundbreaking managing infections innovative strategy minimize impacts while enhancing food security, paving way for more resilient agricultural future.

Language: Английский

Designer antisense circRNAGFP reduces GFP abundance in Arabidopsis protoplasts in a sequence-specific manner, independent of RNAi pathways

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Circular RNAs (circRNAs) are single-stranded RNA molecules characterised by their covalently closed structure and emerging as key regulators of cellular processes in mammals, including gene expression, protein function immune responses. Recent evidence suggests that circRNAs also play significant roles plants, influencing development, nutrition, biotic stress resistance, abiotic tolerance. However, the potential to modulate target abundance plants remains largely unexplored. In this study, we investigated designer using Arabidopsis a model system. We demonstrate treatment with 50 nt circRNA_GFP, containing 30 GFP antisense sequence stretch, results reduced reporter dose- sequence-dependent manner. Notably, open isoform circRNA_GFP had little effect on abundance, indicating importance circular structure. Additionally, mutants defective interference (RNAi), suggesting circRNA activity is independent RNAi pathway. show circRNA, unlike dsRNA, does not induce pattern-triggered immunity (PTI) plants. Findings proof-of-principle study together crucial first steps understanding versatile tools for modulating expression offer exciting prospects application agronomy, particularly enhancing crop traits through metabolic pathway manipulation.

Language: Английский