Synthetic Biology of Natural Products Engineering: Recent Advances Across the Discover–Design–Build–Test–Learn Cycle

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(9), P. 2684 - 2692

Published: Aug. 20, 2024

Advances in genome engineering and associated technologies have reinvigorated natural products research. Here we highlight the latest developments field across discover–design–build–test–learn cycle of bioengineering, from recent progress computational tools for AI-supported mining, enzyme pathway engineering, compound identification to novel host systems new techniques improving production levels, place these trends context responsible research innovation, emphasizing importance anticipatory analysis at early stages process development.

Language: Английский

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Natural Product Chemistry. Reductionism and Biosynthesis

Published: Jan. 1, 2025

Language: Английский

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Promoting the glycosylation of drug-like natural products in a Saccharomyces cerevisiae chassis by deletion of endogenous glycosidases

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132258 - 132258

Published: Feb. 1, 2025

Language: Английский

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Chemoenzymatic synthesis

Communications Chemistry, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 13, 2025

Communications Chemistry is pleased to introduce a Collection of research works focused on recent developments within the interdisciplinary field chemoenzymatic synthesis. Here, Guest Editors highlight key themes and look towards future this field.

Language: Английский

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Mechanism of Unexpected In-Trans Post-PKS Polyketide Reduction in Cochliodone Biosynthesis

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Cochliodone A, a dimeric azaphilone-type compound, is the major product and one of many natural products produced by Chaetomium globosum, filamentous fungus. A exerts antimalarial antimycobacterial activities in addition to being cytotoxic against KB, BC1, NCI-H187 human cancer cell lines. The potential cochliodone its analogues as effective therapeutics cancer, tuberculosis, malaria, together with complex chemical structure, are reasons enough for continued investigation. Here, sequence analyses open reading frames found previously identified biosynthetic gene cluster, series gene-knockout experiments, heterologous vivo production pathway intermediates Aspergillus nidulans vitro assays key enzymes allowed us propose detailed mechanisms leading cochliodones. In addition, we that elimination dimerizing multicopper oxidase CcdJ from ccd led crosstalk between an unrelated benzaldehyde-producing formation generate new secondary metabolites. Most interestingly, however, through study, established sequential actions acetyltransferase, acetate lyase, enoyl reductase achieve full reduction unsaturated backbone polyketide (PKs) generated nonreducing synthase (PKS) CcdL. This alternative mode reducing polyketomethylene chain could be engineered further develop facile chemoenzymatic modification saturation level postpolyketide synthesis trans.

Language: Английский

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Current Approaches and Implications in Discovery of Novel Bioactive Products from Microbial Sources

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(6)

Published: April 22, 2025

Language: Английский

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Excavation of acoustic nanostructures biosynthesis gene clusters by combinatorial strategy

Advanced Biotechnology, Journal Year: 2025, Volume and Issue: 3(2)

Published: May 15, 2025

Abstract Gas vesicles (GVs) produced by microorganisms are genetically engineered, air-filled protein nanostructures that have widespread applications in ultrasound imaging and ultrasound-mediated drug delivery. However, constrained the shape size, most of them difficult to be imaged clinical machines, which limits their biomedical applications. Here, we constructed a hybrid gene cluster structural from Serratia sp. ATCC 39006 accessory Bacillus megaterium Escherichia coli synthesize novel gene-encoded gas vesicle with width approximately 70 nm length about 100 nm, using synthetic biology strategy, termed as ARG S1B . This new type GVs can stably bacteria is able machines vivo vitro. Furthermore, nanostructure easily engineered for different particle sizes through point saturation mutation, expanding sources providing insights into biosynthesis mechanism GVs.

Language: Английский

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Design strategy and preliminary antiproliferative investigation of a modified lignan skeleton derived from aryne and statin

Organic & Biomolecular Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The biological activities of natural products (NPs) provided precious resources for the development new drugs. Numerous studies have shown that statins exhibit cytotoxic potential, which is now an extensive focus investigation. Herein, a remarkably efficient method modification using hexadehydro-Diels-Alder (HDDA) arynes has been described. Notably, lactone, as biologically active group statins, was removed during reaction and novel modified lignan skeleton generated

Language: Английский

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