A prescription for engineering PFAS biodegradation DOI Creative Commons

Lawrence P. Wackett,

Serina L. Robinson

Biochemical Journal, Journal Year: 2024, Volume and Issue: 481(23), P. 1757 - 1770

Published: Nov. 25, 2024

Per- and polyfluorinated chemicals (PFAS) are of rising concern due to environmental persistence emerging evidence health risks humans. Environmental is largely attributed a failure microbes degrade PFAS. PFAS recalcitrance has been proposed result from chemistry, specifically C-F bond strength, or biology, negative selection fluoride toxicity. Given natural evolution many hurdles, this review advocates for strategy laboratory engineering evolution. Enzymes identified participate in defluorination reactions have discovered all Enzyme Commission classes, providing palette metabolic engineering. In vivo biodegradation will require multiple types powerful mitigation mechanisms act concert. The necessary steps to: (1) engineer bacteria that survive very high, unnatural levels fluoride, (2) design, evolve, screen enzymes cleave C–F bonds broader array substrates, (3) create overall physiological conditions make positive selective pressure with substrates.

Language: Английский

Chemoenzymatic synthesis DOI Creative Commons
Paweł Borowiecki, Sandy Schmidt

Communications Chemistry, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 13, 2025

Communications Chemistry is pleased to introduce a Collection of research works focused on recent developments within the interdisciplinary field chemoenzymatic synthesis. Here, Guest Editors highlight key themes and look towards future this field.

Language: Английский

Citations

0
An enzymatic cascade for high-yield and stereoselective synthesis of 4-fluoro-L-threonine DOI
Alberto De Maria, Manuel Nieto‐Domínguez, Phillip T. Lowe

et al.

Chem Catalysis, Journal Year: 2024, Volume and Issue: unknown, P. 101148 - 101148

Published: Oct. 1, 2024

Language: Английский

Citations

0
A prescription for engineering PFAS biodegradation DOI Creative Commons

Lawrence P. Wackett,

Serina L. Robinson

Biochemical Journal, Journal Year: 2024, Volume and Issue: 481(23), P. 1757 - 1770

Published: Nov. 25, 2024

Per- and polyfluorinated chemicals (PFAS) are of rising concern due to environmental persistence emerging evidence health risks humans. Environmental is largely attributed a failure microbes degrade PFAS. PFAS recalcitrance has been proposed result from chemistry, specifically C-F bond strength, or biology, negative selection fluoride toxicity. Given natural evolution many hurdles, this review advocates for strategy laboratory engineering evolution. Enzymes identified participate in defluorination reactions have discovered all Enzyme Commission classes, providing palette metabolic engineering. In vivo biodegradation will require multiple types powerful mitigation mechanisms act concert. The necessary steps to: (1) engineer bacteria that survive very high, unnatural levels fluoride, (2) design, evolve, screen enzymes cleave C–F bonds broader array substrates, (3) create overall physiological conditions make positive selective pressure with substrates.

Language: Английский

Citations

0