Neuroscience Bulletin,
Journal Year:
2023,
Volume and Issue:
39(12), P. 1840 - 1858
Published: Sept. 16, 2023
Abstract
The
mammalian
brain
is
a
highly
complex
network
that
consists
of
millions
to
billions
densely-interconnected
neurons.
Precise
dissection
neural
circuits
at
the
mesoscopic
level
can
provide
important
structural
information
for
understanding
brain.
Optical
approaches
achieve
submicron
lateral
resolution
and
“optical
sectioning”
by
variety
means,
which
has
natural
advantage
allowing
observation
level.
Automated
whole-brain
optical
imaging
methods
based
on
tissue
clearing
or
histological
sectioning
surpass
limitation
depth
in
biological
tissues
delicate
large
volume
tissues.
Combined
with
various
fluorescent
labeling
techniques,
have
shown
great
potential
brain-wide
quantitative
profiling
cells,
circuits,
blood
vessels.
In
this
review,
we
summarize
principles
implementations
some
concepts
regarding
their
future
development.
The Journal of Comparative Neurology,
Journal Year:
2024,
Volume and Issue:
532(1)
Published: Jan. 1, 2024
Abstract
Bone
and
dental
tissues
are
richly
innervated
by
sensory
sympathetic
neurons.
However,
the
characterization
of
morphology,
molecular
phenotype,
distribution
nerves
that
innervate
hard
tissue
has
so
far
mostly
been
limited
to
thin
histological
sections.
This
approach
does
not
adequately
capture
dispersed
neuronal
projections
due
loss
important
structural
information
during
three‐dimensional
(3D)
reconstruction.
In
this
study,
we
modified
immunolabeling‐enabled
imaging
solvent‐cleared
organs
(iDISCO/iDISCO+)
clearing
protocol
image
high‐resolution
structures
in
whole
femurs
mandibles
collected
from
perfused
C57Bl/6
mice.
Axons
their
nerve
terminal
endings
were
immunolabeled
with
antibodies
directed
against
protein
gene
product
9.5
(pan‐neuronal
marker),
calcitonin
gene–related
peptide
(peptidergic
nociceptor
or
tyrosine
hydroxylase
(sympathetic
neuron
marker).
Volume
was
performed
using
light
sheet
fluorescence
microscopy.
We
report
high‐quality
immunolabeling
axons
for
both
neurons
mouse
femur
mandible.
Importantly,
able
follow
through
full
3D
volumes,
highlight
how
extensive
is,
show
regional
differences
innervation
patterns
different
parts
each
bone
(and
surrounding
tissues).
Mapping
axons,
endings,
bony
compartments
may
be
further
elucidating
roles
health
disease.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(6), P. 5167 - 5179
Published: Feb. 1, 2024
Elucidation
of
biological
phenomena
requires
imaging
microenvironments
in
vivo.
Although
the
seamless
visualization
vivo
hypoxia
from
level
whole-body
to
single-cell
has
great
potential
discover
unknown
and
medical
fields,
no
methodology
for
achieving
it
been
established
thus
far.
Here,
we
report
whole-organ
hypoxia,
an
important
microenvironment,
at
resolution
using
activatable
covalent
fluorescent
probes
compatible
with
tissue
clearing.
We
initially
focused
on
overcoming
incompatibility
dyes
refractive
index
matching
solutions
(RIMSs),
which
greatly
hindered
development
molecular
field
The
RIMS
were
then
incorporated
into
hypoxia.
combined
clearing,
comprehensive
single-cell-resolution
a
whole
mouse
body
organs.
Neuroscience Bulletin,
Journal Year:
2023,
Volume and Issue:
39(12), P. 1840 - 1858
Published: Sept. 16, 2023
Abstract
The
mammalian
brain
is
a
highly
complex
network
that
consists
of
millions
to
billions
densely-interconnected
neurons.
Precise
dissection
neural
circuits
at
the
mesoscopic
level
can
provide
important
structural
information
for
understanding
brain.
Optical
approaches
achieve
submicron
lateral
resolution
and
“optical
sectioning”
by
variety
means,
which
has
natural
advantage
allowing
observation
level.
Automated
whole-brain
optical
imaging
methods
based
on
tissue
clearing
or
histological
sectioning
surpass
limitation
depth
in
biological
tissues
delicate
large
volume
tissues.
Combined
with
various
fluorescent
labeling
techniques,
have
shown
great
potential
brain-wide
quantitative
profiling
cells,
circuits,
blood
vessels.
In
this
review,
we
summarize
principles
implementations
some
concepts
regarding
their
future
development.
