Multiphoton fluorescence microscopy for in vivo imaging

Cell, Journal Year: 2024, Volume and Issue: 187(17), P. 4458 - 4487

Published: Aug. 1, 2024

Language: Английский

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Advanced near‐infrared light approaches for neuroimaging and neuromodulation

BMEMat, Journal Year: 2023, Volume and Issue: 1(2)

Published: May 5, 2023

Abstract Almost all physiological processes of animals are controlled by the brain, including language, cognitive, memory, learning, emotion and so forth. Minor brain dysfunction usually leads to diseases disorders. Therefore, it' is greatly meaningful urgent for scientists have a better understanding structure function. Optical approaches can provide powerful tools imaging modulating brain. In particular, optical in near‐infrared (NIR) window (700–1700 nm) exhibit excellent prosperities deep tissue penetration low scattering absorption compared with those visible windows (400–700 nm), which provides promising approach develop desired methods neuroimaging neuromodulation tissues. this review, variable types NIR light neural ions, membrane potential, neurotransmitters, other critical molecules functions summarized. latest breakthrough research regulation NIR‐II (1000–1700 highlighted. Finally, we conclude challenges prospects light‐based both basic further clinical translation.

Language: Английский

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Human REM sleep recalibrates neural activity in support of memory formation

Science Advances, Journal Year: 2023, Volume and Issue: 9(34)

Published: Aug. 25, 2023

The proposed mechanisms of sleep-dependent memory consolidation involve the overnight regulation neural activity at both synaptic and whole-network levels. Now, there is a lack in vivo data humans elucidating if, how, sleep its varied stages balance activity, if such recalibration benefits memory. We combined electrophysiology with two-photon calcium imaging rodents as well intracranial scalp electroencephalography (EEG) to reveal key role for non-oscillatory brain during rapid eye movement (REM) mediate population dynamics. extent this REM predicted success consolidation, expressly modulation hippocampal-neocortical favoring remembering rather than forgetting. findings describe mechanism how human modulates enhance long-term

Language: Английский

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Network state transitions during cortical development

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(8), P. 535 - 552

Published: May 23, 2024

Language: Английский

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Deep-brain optical recording of neural dynamics during behavior

Neuron, Journal Year: 2023, Volume and Issue: 111(23), P. 3716 - 3738

Published: Oct. 6, 2023

Language: Английский

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FIOLA: an accelerated pipeline for fluorescence imaging online analysis

Nature Methods, Journal Year: 2023, Volume and Issue: 20(9), P. 1417 - 1425

Published: Sept. 1, 2023

Language: Английский

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Animal models to study cognitive impairment of chronic kidney disease

AJP Renal Physiology, Journal Year: 2024, Volume and Issue: 326(6), P. F894 - F916

Published: April 18, 2024

Mild cognitive impairment (MCI) is common in people with chronic kidney disease (CKD), and its prevalence increases progressive loss of function. MCI characterized by a decline performance greater than expected for an individual age education level but minimal instrumental activities daily living. Deterioration can affect one or several domains (attention, memory, executive functions, language, perceptual motor social cognition). Given the increasing disease, more CKD will also develop causing enormous burden these individuals, their relatives, society. However, underlying pathomechanisms are poorly understood, current therapies mostly aim at supporting patients lives. This illustrates urgent need to elucidate pathogenesis potential therapeutic targets test novel appropriate preclinical models. Here, we outline necessary criteria experimental modeling disorders CKD. We discuss use mice, rats, zebrafish as model systems present valuable techniques through which function be assessed this setting. Our objective enable researchers overcome hurdles accelerate research aimed improving therapy MCI.

Language: Английский

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Analysis methods for large-scale neuronal recordings

Science, Journal Year: 2024, Volume and Issue: 386(6722)

Published: Nov. 7, 2024

Simultaneous recordings from hundreds or thousands of neurons are becoming routine because innovations in instrumentation, molecular tools, and data processing software. Such can be analyzed with science methods, but it is not immediately clear what methods to use how adapt them for neuroscience applications. We review, categorize, illustrate diverse analysis neural population describe these have been used make progress on longstanding questions neuroscience. review a variety approaches, ranging the mathematically simple complex, exploratory hypothesis-driven, recently developed more established methods. also some common statistical pitfalls analyzing large-scale data.

Language: Английский

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The Impact of Optical Undersampling on the Ca2+ Signal Resolution in Ca2+ Imaging of Spontaneous Neuronal Activity

Journal of Integrative Neuroscience, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 21, 2025

Background: In neuroscience, Ca2+ imaging is a prevalent technique used to infer neuronal electrical activity, often relying on optical signals recorded at low sampling rates (3 30 Hz) across multiple neurons simultaneously. This study investigated whether increasing the rate preserves critical information that may be missed slower acquisition speeds. Methods: Primary cultures were prepared from cortex of newborn pups. Neurons loaded with Oregon Green BAPTA-1 AM (OGB1-AM) fluorescent indicator. Spontaneous activity was (14 and high (500 rates, same (n = 269) analyzed under both conditions. We compared signal amplitude, duration, frequency. Results: Although recurring transients appeared visually similar 14 Hz 500 Hz, quantitative analysis revealed significantly faster rise times shorter durations (half-widths) higher rate. Small-amplitude transients, undetectable became evident particularly in neuropil (putative dendrites axons), but not nearby cell bodies. Large exhibited greater amplitudes temporal dynamics somas, potentially due surface-to-volume ratio dendrites. bulk-loaded OGB1-AM, nucleus-mediated distortions observed every neuron examined 57). Specifically, two regions interest (ROIs) different segments body displayed dye accumulation nucleus. Conclusions: Our findings reveal undersampling leads three types loss: (1) distortion for large-amplitude (2) failure detect small-amplitude bodies, (3) omission neuropil.

Language: Английский

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TWINKLE: An open-source two-photon microscope for teaching and research

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0318924 - e0318924

Published: Feb. 13, 2025

Many laboratories use two-photon microscopy through commercial suppliers, or homemade designs of considerable complexity. The integrated nature these systems complicates customization, troubleshooting, and training on the principles microscopy. Here, we present “Twinkle”: a microscope for Two-photon Imaging in Neuroscience, Kit Learning Education. It is fully open, high performing easy-to-set-up that can effectively be used both education research. instrument features >1 mm field view, using modern objective with 3 working distance 2 inch diameter optics combined GaAsP photomultiplier tubes to maximize fluorescence signal. We document our experiences this system as teaching tool several two week long workshops, exemplify scientific cases, conclude broader note place work growing space open instrumentation.

Language: Английский

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