Optical quasiparticles in paraxial laser beams

Progress in optics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

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Fluorescence lifetime imaging microscopy

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: Nov. 7, 2024

Language: Английский

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Controlling the sound of light: photoswitching optoacoustic imaging

Nature Methods, Journal Year: 2024, Volume and Issue: 21(11), P. 1996 - 2007

Published: Sept. 25, 2024

Language: Английский

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Self‐Driving Microscopes: AI Meets Super‐Resolution Microscopy

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Abstract The integration of Machine Learning (ML) with super‐resolution microscopy represents a transformative advancement in biomedical research. Recent advances ML, particularly deep learning (DL), have significantly enhanced image processing tasks, such as denoising and reconstruction. This review explores the growing potential automation microscopy, focusing on how DL can enable autonomous imaging tasks. Overcoming challenges automation, adapting to dynamic biological processes minimizing manual intervention, is crucial for future microscopy. Whilst still its infancy, revolutionize drug discovery disease phenotyping leading similar breakthroughs been recognized this year's Nobel Prizes Physics Chemistry.

Language: Английский

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Quantum super-resolution imaging: a review and perspective

Nanophotonics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Abstract Quantum super-resolution imaging provides a nonlabeling method to surpass the diffraction limit of systems. This technique relies on measurement second-order correlation function and usually employs spatially entangled photon sources. We introduce recent methods that achieve spatial resolution enhancement through quantum approaches, particularly techniques utilizing biphoton states. The fundamental mechanisms are discussed in detail explain why states enable super-resolution. Additionally, we multiple algorithms extract from readings two-dimensional detectors. Several cases reviewed evaluate advantages prospects imaging, along with discussion practical developments potential applications.

Language: Английский

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MINFLUX Nanoscopy: A “Brilliant” Technique Promising Major Breakthrough

Microscopy Research and Technique, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

ABSTRACT MINFLUX nanoscopy relies on the localization of single fluorophores with expected ~ 2 nm precision in 3D mapping, roughly one order magnitude better than standard stimulated emission depletion microscopy or stochastic optical reconstruction microscopy. This “brilliant” technique takes advantage specialized principles and algorithms that require only dim fluorescence signals a minimum flux photons; hence name follows. With this level performance, imaging tracking should allow for routine study biological processes down to molecular scale, revealing previously unresolved details cell structures, such as organization calcium channels muscle cells clustering receptors synapses. Whereas high is definitely strength technique, limitations challenges also exist, especially labeling procedures aiming at appropriate density on/off switching kinetics. primer presents some significant results achieved so far highlights specific operational crucial technique.

Language: Английский

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Microscopy methods for the in vivo study of nanoscale nuclear organization

Biochemical Society Transactions, Journal Year: 2025, Volume and Issue: 53(01)

Published: Feb. 3, 2025

Eukaryotic genomes are highly compacted within the nucleus and organized into complex 3D structures across various genomic physical scales. Organization plays a key role in gene regulation, both facilitating regulatory interactions to promote transcription while also enabling silencing of other genes. Despite functional importance genome organization determining cell identity function, investigating nuclear this wide range scales has been challenging. Microscopy provides opportunity for direct visualization pioneered discoveries field. Nonetheless, nanoscale nucleus, such as nucleosomes chromatin loops, requires super-resolution imaging go beyond ~220 nm diffraction limit. Here, we review recent advances technology their promise uncover new insights at nanoscale. We discuss different modalities how they have applied with focus on light microscopy its application vivo systems. Finally, conclude our perspective continued technical innovations will advance understanding structure function.

Language: Английский

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Biocompatible Sulfonium-Based Covalent Probes For Endogenous Tubulin Fluorescence Nanoscopy In Live And Fixed Cells

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Fluorescent probes enable the visualization of dynamic cellular processes with high precision, particularly when coupled super-resolution imaging techniques that surpass diffraction limit. Traditional methods include fluorescent protein fusion (e.g., GFP) or organic fluorophores linked to ligands targeting interest. However, these approaches often introduce functional disruptions ligand-associated biological effects. Herein, we address challenges by developing covalent for endogenous tubulin, a critical cytoskeletal involved in such as cell movement, division, and biomolecule trafficking. Using well-known tubulin binder cabazitaxel permeable fluorophore silicon-rhodamine basis, novel biocompatible cleavable linker containing sulfonium center. This allowed construction optimized probe, 6-SiR-o-C₉-CTX, demonstrating excellent permeability, fluorogenic properties, ability covalently label across various human lines. Importantly, moiety could be washed out while preserving staining, ensuring minimal disruption function. labeling technique is compatible STED nanoscopy both live fixed cells, offering powerful high-resolution tool.

Language: Английский

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Elegant high-order harmonic vortices generation

Applied Physics Letters, Journal Year: 2025, Volume and Issue: 126(8)

Published: Feb. 24, 2025

High-order harmonic generation is a cornerstone of attosecond science, with applications spanning from spectroscopy to the creation ultrashort light pulses temporal duration falling in regime. In addition, beams carrying orbital angular momentum (OAM) allow studies light–matter interactions mediated by OAM couplings. this work, we present an alternative approach generating high-order vortices using elegant Laguerre–Gaussian (eLG) beams. We examine spatiotemporal characteristics these far-field regime and demonstrate how low divergence eLG makes them suitable for producing extreme ultraviolet (XUV) twisted pulses. Additionally, solving Fraunhofer integral, analyze influence azimuthal radial indices on spatial profile vortex beams, thereby exploring impact larger topological charges. This study extends concept generated beyond paraxial approximation.

Language: Английский

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Guidelines for MINFLUX Excitation Pattern Design

ACS Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

Language: Английский

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