Spatiotemporal Dynamics of Human Ovarian Cortex Transcriptome Following Vitrification and Thawing: Insights into the FOS/AP-1 Pathway DOI
Wen Li,

Fanghao Guo,

Di Sun

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Abstract Ovary is a vital and dynamic reproductive organ. Ovarian tissue cryopreservation (OTC) plays role in female fertility preservation, especially for adolescent cancer patients. Yet, the sensitive cell populations cryoinjury molecular mechanisms underlying remain elusive. This study employs single-cell RNA sequencing spatial transcriptomics to dissect impacts of temperature stress cryoprotectant toxicity on human ovarian cortex. The spatiotemporal characteristics eight types following vitrification-thawing had been comprehensively characterized. Granulosa, perivascular stromal cells are identified as most OTC procedure. Further analysis using Smart-seq2 oocytes highlights alterations in“cell cycle” “DNA methylation” pathways. Notably, FOS/AP-1 pathway emerges crucial response factor during cryopreservation. Inhibition this with T-5224 mitigates vascular damage reduces apoptosis vitrification-thawed ovaries. These findings provide insight into dynamics vitrification thawing, aiding prioritizing therapeutic strategies pre- post-cryopreservation interventions.

Language: Английский

Single-cell resolution reveals the cell types and transcriptomic landscape of the ovary in mandarin fish (Siniperca chuatsi) DOI

Siling Zhang,

Jie Ren,

Shanwen Jiang

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742430 - 742430

Published: March 1, 2025

Language: Английский

Citations

0
Absence of CDK12 in oocyte leads to female infertility DOI Creative Commons
Denisa Jansová,

Veronika Sedmikova,

Fatima J. Berro

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 27, 2025

Language: Английский

Citations

0
Ovarian vascular aging: a hidden driver of mid-age female fertility decline DOI Creative Commons
Ge Wang,

Ruihua Yang,

Hua Zhang

et al.

npj Aging, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 30, 2025

Although ovarian reserve remains sufficient, function declines in mid-age, leading to reduced fertility around age 35, with the causes remaining unclear. Recent studies highlight vascular aging as a key factor this decline, age-related reductions remodeling disrupting oocyte development. Salidroside, natural compound that reverses and promotes angiogenesis, presents promising strategy rejuvenate health enhance fertility, offering potential for preserving reproductive aged women.

Language: Английский

Citations

0
SIRT7 links H3K36ac epigenetic regulation with genome maintenance in the aging mouse testis DOI
Anna Guitart-Solanes,

Mayra Romero,

Irene Fernández-Duran

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Reproductive aging is an increasing health concern affecting family planning and overall well-being. While extensively studied in females, the mechanisms driving male reproductive remain largely unexamined. Here we found that mammalian Sirtuin 7 (SIRT7) sustains spermatogenesis age-dependent manner through control of histone 3 lysine 36 acetylation (H3K36ac). SIRT7 deficiency mice resulted increased levels H3K36ac spermatogonia spermatocytes. In a germ cell line, disrupted nucleosome stability vulnerability to genotoxic stress. Importantly, undifferentiated spermatogonia, which are required for continuous sperm production, decreased prematurely Sirt7 -/- showed genome damage accumulation. These changes were concurrent with defects homologous chromosome synapsis partial meiotic arrest. Taken together, our results indicate connects epigenetic regulation long-term cells, ensuring steady-state during lengthy lifespan.

Language: Английский

Citations

0
Spatiotemporal Dynamics of Human Ovarian Cortex Transcriptome Following Vitrification and Thawing: Insights into the FOS/AP-1 Pathway DOI
Wen Li,

Fanghao Guo,

Di Sun

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Abstract Ovary is a vital and dynamic reproductive organ. Ovarian tissue cryopreservation (OTC) plays role in female fertility preservation, especially for adolescent cancer patients. Yet, the sensitive cell populations cryoinjury molecular mechanisms underlying remain elusive. This study employs single-cell RNA sequencing spatial transcriptomics to dissect impacts of temperature stress cryoprotectant toxicity on human ovarian cortex. The spatiotemporal characteristics eight types following vitrification-thawing had been comprehensively characterized. Granulosa, perivascular stromal cells are identified as most OTC procedure. Further analysis using Smart-seq2 oocytes highlights alterations in“cell cycle” “DNA methylation” pathways. Notably, FOS/AP-1 pathway emerges crucial response factor during cryopreservation. Inhibition this with T-5224 mitigates vascular damage reduces apoptosis vitrification-thawed ovaries. These findings provide insight into dynamics vitrification thawing, aiding prioritizing therapeutic strategies pre- post-cryopreservation interventions.

Language: Английский

Citations

0