A quantitative spatial cell-cell colocalizations framework enabling comparisons between in vitro assembloids and pathological specimens

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 6, 2025

Spatial omics is enabling unprecedented tissue characterization, but the ability to adequately compare spatial features across samples under different conditions lacking. We propose a quantitative framework that catalogs significant, normalized, colocalizations between pairs of cell subpopulations, comparisons among variety biological samples. perform cell-pair colocalization analysis on multiplexed immunofluorescence images assembloids constructed with lung adenocarcinoma (LUAD) organoids and cancer-associated fibroblasts derived from human tumors. Our data show recapitulate LUAD tumor-stroma organization, justifying their use as tool for investigating biology disease. Intriguingly, drug-perturbation studies identify drug-induced rearrangements also appear in treatment-naïve tumor samples, suggesting potential directions characterizing (re)-organization related drug resistance. Moreover, our work provides an opportunity quantify common goal building associated disease processes response.

Language: Английский

From biology of ageing to geroscience: where will knowledge take us?

Age and Ageing, Journal Year: 2025, Volume and Issue: 54(4)

Published: March 28, 2025

Abstract Healthy life expectancy is a major challenge in many countries and one of the World Health Organisation’s main concerns for current decade. With different animal models, from invertebrates to mammals, research into biology ageing has identified various biological physiological processes that alter quality ageing. Twelve characteristics have been defined, aim growing number studies find how slow down or halt their onset. Unfortunately, direct transposition models humans too often disappointing, race bring anti-ageing products market source misleading promises. The development geroscience will enable identification validation, with more relevant clinical evidence, pro-ageing targets develop therapies healthy

Language: Английский

Decoding the crossroads of aging and cancer through single‐cell analysis: Implications for precision oncology

International Journal of Cancer, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Abstract Single‐cell analysis is a transformative approach to understanding cellular heterogeneity in aging and cancer, interconnected processes driven by mechanisms like senescence immune modulation. This review explores how influences cancer initiation, progression, treatment resistance within the tumor microenvironment (TME). By examining recent studies using single‐cell technologies, we reveal nuanced roles of tumorigenesis, interactions, therapeutic outcomes. Aging closely tied with senescent cells demonstrating heightened proliferative, invasive, metastatic capabilities. Emerging senolytic therapies targeting aging‐related pathways hold promise for enhancing efficacy. Advanced tools such as spatial transcriptomics, molecular probes, artificial intelligence further refine our TME. integrating these future research can clarify intricate interactions between advancing precision oncology improving outcomes patients.

Language: Английский