Metagenomic analysis reveals microbial drivers of heat resistance in dairy cattle DOI Creative Commons
Mingxun Li, Zhiwei Wang, Zheng Ma

et al.

Animal Microbiome, Journal Year: 2025, Volume and Issue: 7(1)

Published: April 9, 2025

Heat stress poses a significant challenge to dairy cattle, leading adverse physiological effects, reduced milk yield, impaired reproduction performance and economic losses. This study investigates the role of rumen microbiome in mediating heat resistance cows. Using entropy-weighted TOPSIS method, we classified 120 cows into heat-resistant (HR) heat-sensitive (HS) groups based on biochemical markers, including rectal temperature (RT), respiratory rate (RR), salivation index (SI) serum levels potassium ion (K+), shock protein 70 (HSP70) cortisol. Metagenomic sequencing fluid samples revealed distinct microbial compositions functional profiles between two groups. HR exhibited more cohesive functionally stable microbiome, dominated by taxa such as Ruminococcus flavefaciens Succiniclasticum, which are key players fiber degradation short-chain fatty acid production. Functional analysis highlighted enrichment pentose phosphate pathway (PPP) cows, suggesting metabolic adaptation that enhances oxidative management. In contrast, HS showed increased activity tricarboxylic (TCA) cycle, pyruvate metabolism other energy-intensive pathways, indicating higher burden under stress. These findings underscore critical modulating suggest potential microbiome-based strategies for improving cattle resilience climate change.

Language: Английский

Altered oral health and microbiota in drug-free patients with schizophrenia DOI Creative Commons
Hua‐Wei Huang,

Nai-yan Yang,

Mianmian Chen

et al.

BMC Psychiatry, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 25, 2025

The oral microbiota is associated with neuro-psychiatric disorders. However, there presently inadequate comprehension regarding the correlation between schizophrenia and microbiota. Moreover, patients frequently exhibit poor health, potentially influencing research outcomes. Therefore, this study aims to investigate changes in health status drug-free patients. Oral samples were collected from 50 healthy controls (HCs). downstream analysis was based on Illumina sequencing of V3-V4 hypervariable region 16 S rRNA gene. alpha diversity SCZ group increased, such as Shannon index (p < 0.001) Simpson = 0.004), while community structure also displays variance compared HC 0.001). Key discriminative taxa found LEfSe analysis, including phyla Fusobacteriota, Firmicutes, Actinobacteriota. differential microbial functions showed a strong clinical conditions. Further demonstrated that models entire effectively distinguished (AUC 0.97). significant Drug-free appear be closely environment. Sample: HCs & recruited reduce medication impact. Employed comprehensive assessments sequencing. poorer altered correlated

Language: Английский

Citations

0
Metagenomic analysis reveals microbial drivers of heat resistance in dairy cattle DOI Creative Commons
Mingxun Li, Zhiwei Wang, Zheng Ma

et al.

Animal Microbiome, Journal Year: 2025, Volume and Issue: 7(1)

Published: April 9, 2025

Heat stress poses a significant challenge to dairy cattle, leading adverse physiological effects, reduced milk yield, impaired reproduction performance and economic losses. This study investigates the role of rumen microbiome in mediating heat resistance cows. Using entropy-weighted TOPSIS method, we classified 120 cows into heat-resistant (HR) heat-sensitive (HS) groups based on biochemical markers, including rectal temperature (RT), respiratory rate (RR), salivation index (SI) serum levels potassium ion (K+), shock protein 70 (HSP70) cortisol. Metagenomic sequencing fluid samples revealed distinct microbial compositions functional profiles between two groups. HR exhibited more cohesive functionally stable microbiome, dominated by taxa such as Ruminococcus flavefaciens Succiniclasticum, which are key players fiber degradation short-chain fatty acid production. Functional analysis highlighted enrichment pentose phosphate pathway (PPP) cows, suggesting metabolic adaptation that enhances oxidative management. In contrast, HS showed increased activity tricarboxylic (TCA) cycle, pyruvate metabolism other energy-intensive pathways, indicating higher burden under stress. These findings underscore critical modulating suggest potential microbiome-based strategies for improving cattle resilience climate change.

Language: Английский

Citations

0