Clinical validation of peripheral blood mononuclear cell DNA methylation markers for accurate early detection of hepatocellular carcinoma in Asian patients DOI Creative Commons
David Cheishvili,

Chifat Wong,

Mohammad Mahbubul Karim

et al.

Communications Medicine, Journal Year: 2024, Volume and Issue: 4(1)

Published: Oct. 30, 2024

Hepatocellular carcinoma (HCC), a leading cause of cancer-related deaths globally, poses significant challenges in early detection. Improved diagnostic accuracy can drastically influence patient outcomes, emphasizing the need for innovative, non-invasive biomarkers. This study utilized cohort 402 participants, including healthy controls, chronic hepatitis patients, and HCC patients from Bangladesh, to evaluate DNA methylation signatures peripheral blood mononuclear cells (PBMC). We performed targeted next-generation sequencing on selected genes previously identified assess their dynamics. The development M8 M4 scores was based these dynamics, using Receiver Operating Characteristic (ROC) analysis determine effectiveness detecting early-stage alongside existing markers such as epiLiver alpha-fetoprotein (AFP). Integration with AFP significantly enhances sensitivity HCC. M4+epiLiver score achieves 79.4% Stage A HCC, while combining increases 88.2–95.7% across all stages, indicating superior performance compared each marker used alone. Our confirms that gene profiles established substantially improves integrated approach holds promise advancing cancer diagnostics, potentially earlier treatment interventions improved survival rates high-risk patients. Cheishvili et al. investigate use detection hepatocellular an Asian cohort. demonstrates biomarkers accurately distinguish between controls different stages disease. Liver is one top causes death worldwide, finding it crucial successful treatment. research focuses simple test look specific changes signal liver cancer. tested this method diverse group people those already at high risk due infections. By new other tests, we were able detect more than by traditional methods could make easier less invasive find early, offering better chance effective hopeful prognosis risk.

Language: Английский

Clinical validation of peripheral blood mononuclear cell DNA methylation markers for accurate early detection of hepatocellular carcinoma in Asian patients DOI Creative Commons
David Cheishvili,

Chifat Wong,

Mohammad Mahbubul Karim

et al.

Communications Medicine, Journal Year: 2024, Volume and Issue: 4(1)

Published: Oct. 30, 2024

Hepatocellular carcinoma (HCC), a leading cause of cancer-related deaths globally, poses significant challenges in early detection. Improved diagnostic accuracy can drastically influence patient outcomes, emphasizing the need for innovative, non-invasive biomarkers. This study utilized cohort 402 participants, including healthy controls, chronic hepatitis patients, and HCC patients from Bangladesh, to evaluate DNA methylation signatures peripheral blood mononuclear cells (PBMC). We performed targeted next-generation sequencing on selected genes previously identified assess their dynamics. The development M8 M4 scores was based these dynamics, using Receiver Operating Characteristic (ROC) analysis determine effectiveness detecting early-stage alongside existing markers such as epiLiver alpha-fetoprotein (AFP). Integration with AFP significantly enhances sensitivity HCC. M4+epiLiver score achieves 79.4% Stage A HCC, while combining increases 88.2–95.7% across all stages, indicating superior performance compared each marker used alone. Our confirms that gene profiles established substantially improves integrated approach holds promise advancing cancer diagnostics, potentially earlier treatment interventions improved survival rates high-risk patients. Cheishvili et al. investigate use detection hepatocellular an Asian cohort. demonstrates biomarkers accurately distinguish between controls different stages disease. Liver is one top causes death worldwide, finding it crucial successful treatment. research focuses simple test look specific changes signal liver cancer. tested this method diverse group people those already at high risk due infections. By new other tests, we were able detect more than by traditional methods could make easier less invasive find early, offering better chance effective hopeful prognosis risk.

Language: Английский

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