Comparative Analysis of the Human Proteome Profile in Visceral Adipose and Liver Tissue in Individuals with Obesity with and Without MASLD and MASH DOI Creative Commons
Julie Steen Pedersen, Lili Niu, Nicolai J. Wewer Albrechtsen

et al.

Livers, Journal Year: 2025, Volume and Issue: 5(2), P. 16 - 16

Published: April 10, 2025

Background/Objectives: Visceral adipose tissue (VAT) may play a direct role in the development of metabolic dysfunction-associated steatotic liver disease (MASLD) and its progression to steatohepatitis (MASH). In this study, we employed untargeted proteomics analyses on paired biopsies from VAT tissues patients with obesity, MASLD, MASH. Our objective was investigate tissue-specific protein expression patterns search potential proteomic signature associated MASH both tissue. Methods: were collected 70 subjects severe obesity (SWOs) nine control study without (CON). SWOs stratified basis histology into LS− (no steatosis), LS+ (liver Peptides extracted frozen analyzed by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS). Raw files Spectronaut, proteins searched against human FASTA Uniprot database, significantly expressed two analyzed. The p-values false discovery rate (FDR) corrected. Results: A total 59 42 differentially between four groups: LS−, LS+, MASH, CON. majority upregulated, many related lipid metabolism. VAT, only one protein, mitochondrial sulfide:quinone oxidoreductase (SQOR), downregulated group only. six altered compared three other groups. Correlation top 10 positive dominated inflammatory detoxification proteins. Conclusions: presence not reflected proteome, proteome generally affected more than MASLD severity. Several immunomodulating correlated could reflect common pathophysiological characteristics.

Language: Английский

Comparative Analysis of the Human Proteome Profile in Visceral Adipose and Liver Tissue in Individuals with Obesity with and Without MASLD and MASH DOI Creative Commons
Julie Steen Pedersen, Lili Niu, Nicolai J. Wewer Albrechtsen

et al.

Livers, Journal Year: 2025, Volume and Issue: 5(2), P. 16 - 16

Published: April 10, 2025

Background/Objectives: Visceral adipose tissue (VAT) may play a direct role in the development of metabolic dysfunction-associated steatotic liver disease (MASLD) and its progression to steatohepatitis (MASH). In this study, we employed untargeted proteomics analyses on paired biopsies from VAT tissues patients with obesity, MASLD, MASH. Our objective was investigate tissue-specific protein expression patterns search potential proteomic signature associated MASH both tissue. Methods: were collected 70 subjects severe obesity (SWOs) nine control study without (CON). SWOs stratified basis histology into LS− (no steatosis), LS+ (liver Peptides extracted frozen analyzed by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS). Raw files Spectronaut, proteins searched against human FASTA Uniprot database, significantly expressed two analyzed. The p-values false discovery rate (FDR) corrected. Results: A total 59 42 differentially between four groups: LS−, LS+, MASH, CON. majority upregulated, many related lipid metabolism. VAT, only one protein, mitochondrial sulfide:quinone oxidoreductase (SQOR), downregulated group only. six altered compared three other groups. Correlation top 10 positive dominated inflammatory detoxification proteins. Conclusions: presence not reflected proteome, proteome generally affected more than MASLD severity. Several immunomodulating correlated could reflect common pathophysiological characteristics.

Language: Английский

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