Genomic data reveals habitat partitioning in massive Porites on Guam, Micronesia

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 24, 2024

Corals in marginal reef habitats generally exhibit less bleaching and associated mortality compared to nearby corals more pristine environments. It is unclear, however, if these differences are due environmental differences, including turbidity, or genomic between the coral hosts different One particularly interesting case genus Porites, which contains numerous morphologically similar massive Porites species inhabiting a wide range of habitats, from turbid river deltas stagnant back reefs high-energy fore reefs. Here, we generate ddRAD data for 172 delta adjacent (<0.5 km) populations on Guam assess extent genetic differentiation among two contrasting environments throughout island. Phylogenetic population analyses consistently identify seven clades with largest predominantly either reefs, respectively. No structure was detected clades, Cladocopium dominant symbiont all The perceived resilience may therefore be attributed interspecific species, addition potentially mediating differences. Marginal not provide suitable refuge many heating world, but instead host additional cryptic diversity.

Language: Английский

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Distinct modes of holobiont specialization among cryptic coral lineages

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 6, 2024

Abstract As ocean warming threatens reefs worldwide, identifying corals with adaptations to higher temperatures is critical for conservation. Genetically distinct but morphologically similar ( i.e., cryptic) coral populations can be specialized extreme habitats and thrive under stressful conditions. These often associate locally beneficial microbiota (Symbiodiniaceae photobionts bacteria), clouding interpretation of the drivers thermal tolerance. Here, we leverage a holobiont (massive Porites ) high host-partner fidelity investigate adaptive variation across classic (“typical” conditions) characterized by light attenuation. We uncovered three cryptic lineages that exhibit limited micro-morphological variation; one lineage dominated (L1), had more even distributions (L2), third was restricted (L3). Two were closely related ∼4300 km away, suggesting these are widespread. All harbored Cladocopium C15 photobionts, strain-level compositions differed among reef types. L1 associated bacteria in each type, whereas L2 relatively stable associations. L3 hosted unique photobiont strains, signaling host-photobiont fidelity. Analysis harvesting capacity tolerance revealed key underpinning survival habitats. highest absorption efficiency lowest tolerance, it specialist. showing an intermediate habitat generalism, potentially explaining how survives well both findings reveal diverging strategies cope Resolving understanding populations; uncovering thermally-tolerant holobionts strengthen our evolution symbiosis, support global conservation restoration efforts.

Language: Английский

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Genome-resolved diversity and biosynthetic potential of the coral reef microbiome

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 18, 2024

Coral reefs are marine biodiversity hotspots that provide a wide range of ecosystem services. They also reservoirs bioactive compounds, many which produced by microbial symbionts associated with reef invertebrate hosts. However, for the keystone species coral reefs, reef-building corals themselves, we still lack systematic assessment their microbially encoded biosynthetic potential, and thus molecular resources may be at stake due to alarming decline in cover. Here, analysed genomes reconstructed from 820 samples three representative genera collected 99 across 32 islands during two-year expedition throughout Pacific Ocean (Tara Pacific). By contextualising our analyses microbiomes other species, found genomic information was previously available only 10% 4,224 overall less than 1% 645 exclusively identified Tara samples. We host-specific potential rival or even surpass traditional targets natural product discovery, such as sponges soft corals. Fire were not particularly diverse gene clusters (BGCs), but BGC-rich bacteria, including Acidobacteriota spp., have been recently highlighted promising repertoire. Together, this study unveils new candidate sources compound prioritises isolation, underscores importance conservation efforts linking macro-organismal loss biotechnological potential.

Language: Английский

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Multi-omics determination of metabolome diversity in natural coral populations in the Pacific Ocean

Communications Earth & Environment, Journal Year: 2023, Volume and Issue: 4(1)

Published: Aug. 8, 2023

Abstract Coral reefs are considered one of the most emblematic ecosystems in our oceans, but their existence is increasingly threatened by climate change. In this study, natural populations two reef-building coral genera, Pocillopora spp. and Porites spp., hydrocoral Millepora cf. platyphylla from different marine provinces Pacific Ocean were investigated using a multi-omics approach as part Tara expedition. Here, we propose standardised method consisting biphasic extraction followed metabolomics analysis mass spectrometry for lipidome 1 H nuclear magnetic resonance hydrophilic metabolites. Our study assessed broad range metabolome first to identify add 24 compounds NMR over 200 lipids MS analyses corals. Metabolic profiles distinct among genera not within genotypes cnidarian Although endosymbiotic dinoflagellates family Symbiodiniaceae known play central role metabolomic signature holobiont, they did account all differences. This suggests that combined effect members holobiont an interaction with environment might be at play. provides foundational knowledge on metabolome.

Language: Английский

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Population genetic differentiation of the ubiquitous brooding coral Pocillopora acuta along Phuket Island reefs in the Andaman Sea, Thailand

BMC Ecology and Evolution, Journal Year: 2023, Volume and Issue: 23(1)

Published: Aug. 26, 2023

Abstract Background The widespread Indo-Pacific coral species Pocillopora acuta Lamarck, 1816 displays varying levels of asexual versus sexual reproduction, with strong repercussions on genetic diversity, connectivity and structuring within among populations. For many geographic regions, baseline information diversity is still lacking, particularly in the Andaman Sea. region suffered a massive heat-induced bleaching event 2010 high cover loss branching such as P. . A subsequent 2016, however, revealed mild response pocilloporids compared to other taxa region, suggesting that rare, heat tolerant genotypes had been selected by event. In order test whether this potential ‘evolutionary rescue’ has led low we conducted population survey covering total nine different populations (336 individuals) along 50 km coastal stretch around Phuket Island, Thailand. We used six microsatellite markers assess genotypic determine prevalent mode reproduction (i.e. or recruitment). Results contrast Indian Ocean populations, majority corals study adopted (75% across all populations). At same time, substantial regional gene flow was observed Island differentiation indicated three clusters were separated only few kilometers. Patterns isolation distance over 0.7 – 40 suggest small-scale barriers, changing currents throughout each monsoonal season, potentially contributing locally restricted dispersal larvae. Conclusions occurrence distinct short stretches suggests not extreme impoverishment. While more in-depth genomic analyses are necessary investigate changes following events, our results will help guide conservation efforts maintain likely be dominant future, warmer Sea reefs.

Language: Английский

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Dominance of the coral Pocillopora acuta around Phuket Island in the Andaman Sea, Thailand

Ecology and Evolution, Journal Year: 2023, Volume and Issue: 13(11)

Published: Nov. 1, 2023

Abstract Pocillopora damicornis (Linnaeus, 1758), a species complex, consists of several genetic lineages, some which likely represent reproductively isolated species, including the acuta Lamarck, 1816. can exhibit similar morphological characteristics as P. , thus making it difficult to identify species‐level taxonomic units. To determine whether ‐like colonies on reefs in Andaman Sea (previously often identified ) consist different we sampled individual at five sites along 50 km coastal stretch Phuket Island and four island towards Krabi Province, Thailand. We sequenced 210 coral samples for mitochondrial open reading frame six distinct haplotypes, all belonging according literature. Recently, was observed efficiently recolonize heat‐damaged Thailand well globally, potentially important future reefs. Specifically light global change, this study underscores importance high‐resolution molecular recognition, since units are factors population studies, latter crucial management conservation efforts.

Language: Английский

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Isolation by disturbance: a pattern of genetic structure of the coral Pocillopora grandis in the Gulf of California

Marine Ecology Progress Series, Journal Year: 2024, Volume and Issue: 733, P. 43 - 57

Published: Feb. 20, 2024

Genetic differentiation within species can be influenced by the distance between populations (isolation distance, IBD) or differences in habitats they occupy environment, IBE). Understanding population structure and dynamics that influence genetic diversity small (<100 km 2 ) multi-use marine protected areas (MPAs) varying disturbances protections is relevant to better designing managing them. Pocillopora grandis communities were studied at 10 different sites Gulf of California, Mexico. The comparison inside outside an MPA whether followed IBD IBE was investigated with marker HSP70B. Environmental heterogeneity used test for IBE, measured comparing present benthic site through analysis satellite images, while IBD. Mantel tests found P. did not follow IBE. Instead, cluster grouped low (no-take zones) high (crown-of-thorns starfish outbreaks highly visited tourists) disturbance together remaining another (ΦRT = 0.082, p 0.007). Furthermore, lower disturbance. Our results suggest instead influencing structure, protection levels could have a correlation patterns (i.e. isolation disturbance, ID). These findings will valuable MPAs terms efficient management objectives considering appropriate spacing protections, depending on how vulnerable

Language: Английский

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Genomic data reveals habitat partitioning in massivePoriteson Guam, Micronesia

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

Abstract Corals in marginal reef habitats generally exhibit less bleaching and associated mortality compared to nearby corals more pristine environments. It is unclear, however, if these differences are due environmental differences, including turbidity, or genomic between the coral hosts different One particularly interesting case genus Porites , which contains numerous morphologically similar massive species inhabiting a wide range of habitats, from turbid river deltas stagnant back reefs high-energy fore reefs. Here, we generate ddRAD data for 172 delta adjacent populations on Guam assess extent genetic differentiation among two contrasting environments throughout island. Phylogenetic population analyses identify seven clades with largest predominantly either reefs, respectively. No structure was detected clades, Cladocopium dominant symbiont all The perceived resilience may therefore be attributed interspecific species, addition potentially mediating differences. Marginal not provide suitable refuge many heating world, but instead host additional cryptic diversity.

Language: Английский

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Niche breadth and divergence in sympatric cryptic coral species (Pocillopora spp.) across habitats within reefs and among algal symbionts

Evolutionary Applications, Journal Year: 2024, Volume and Issue: 17(8)

Published: Aug. 1, 2024

While the presence of morphologically cryptic species is increasingly recognized, we still lack a useful understanding what causes and maintains co-occurring its consequences for ecology, evolution, conservation communities. We sampled 724

Language: Английский

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Holobiont Traits Shape Climate Change Responses in Cryptic Coral Lineages

Global Change Biology, Journal Year: 2024, Volume and Issue: 30(11)

Published: Nov. 1, 2024

ABSTRACT As ocean warming threatens reefs worldwide, identifying corals with adaptations to higher temperatures is critical for conservation. Genetically distinct but morphologically similar (i.e. cryptic) coral populations can be specialized extreme habitats and thrive under stressful conditions. These often associate locally beneficial microbiota (Symbiodiniaceae photobionts bacteria), obscuring the main drivers of thermal tolerance. Here, we leverage a holobiont (massive Porites ) high fidelity C15 investigate adaptive variation across classic (“typical” conditions) characterized by light attenuation. We uncovered three cryptic lineages that exhibit limited micro‐morphological variation; one lineage dominated (L1), had more even distributions (L2), third was restricted (L3). L1 L2 were closely related ~4300 km away, suggesting some are widespread. All harbored Cladocopium photobionts; shared photobiont pool differed in composition between reef types, yet L3 mostly unique strains not found other lineages. Assemblages bacterial partners among types lineage‐specific ways, employ microbiome regulation strategies. Analysis light‐harvesting capacity tolerance revealed underpinning survival habitats: highest absorption efficiency lowest tolerance, it specialist. showing an intermediate generalist lineage. findings reveal diverging strategies cope Resolving key understanding populations, strengthen our evolution symbiosis, support global conservation restoration efforts.

Language: Английский

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