Wastewater surveillance for antibiotics and resistance genes in a river catchment: Spatiotemporal variations and the main drivers

Water Research, Journal Year: 2023, Volume and Issue: 251, P. 121090 - 121090

Published: Dec. 29, 2023

Language: Английский

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Targeted Enrichment Sequencing Utilizing a Respiratory Pathogen Panel for Genomic Wastewater-Based Viral Epidemiology in Uruguay

Food and Environmental Virology, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 9, 2025

Language: Английский

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Aquasearch: Enhancing Green Environmental Proteomics via MALDI-TOF-based Sewage Water Profiling

Green Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 100205 - 100205

Published: Jan. 1, 2025

Language: Английский

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Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 30, 2025

The CRISPR-based detection methods have been widely applied, yet they remain limited by the non-universal nature of one-pot diagnostic approaches. Here, we report a universal fluorescent method for epidemic pathogens, delivering results within 15-20 min. This uses heparin sodium to precisely tunes cis-cleavage capability Cas12 via interference with Cas12a-crRNA binding process, thereby generating significant fluorescence due accumulation isothermal amplification products. Additionally, this assay accommodates both classic and suboptimal PAMs, as well various Cas12a subtypes such LbCas12a, AsCas12a, AapCas12b. Such robust demonstrates sensitivity specificity exceeding 95% in monkeypox pseudovirus, influenza A virus, SARS-CoV-2 from saliva or wastewater samples, when compared qPCR RT-qPCR. Moreover, cost per thousand is $0.01 $0.04 only. Collectively, fast approach based on offers potential possibilities point-of-care testing.

Language: Английский

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Impact of physicochemical parameters on nicotine metabolites and Bisphenol A in municipal wastewater: A pilot study in a German metropolitan area

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 967, P. 178767 - 178767

Published: Feb. 12, 2025

Wastewater-based epidemiology (WBE) is a promising approach for assessing population-level exposures to toxicants and lifestyle factors. Our pilot investigated the spatial variability of biomarkers indicating exposure nicotine endocrine disrupting chemicals in municipal wastewater, taking into account important covariates, i.e. physicochemical wastewater parameters sewershed characteristics. We also tested normalization by an endogenous population marker. Twenty-four-hour composite samples (n = 24) were collected volume-proportional manner using automated samplers from four treatment plants Essen, Germany, over three weeks late 2023. Substances public health concern, previously already quantified expected be present high concentrations, analyzed SPE-LC-HRMS. Concentrations normalized reported (de jure) daily mass load catecholamine metabolite vanillylmandelic acid (VMA) as proxy de facto population. Spatial variations multiple linear regression. Nicotine metabolites (cotinine, hydroxycotinine) industrial chemical Bisphenol A (BPA) quantifiable all samples. The population-normalized (median range: 0.6-1.6 g/d/1000 inh) BPA (2.5-4.0 varied between WWTPs, but observed variation disappeared when adjusting temperature (nicotine) pH (BPA). Normalization VMA resulted different patterns increased variance considerably. findings highlight importance characteristics analyzing community-wide differences factors via ensure accurate interpretations underlying drivers these differences.

Language: Английский

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Sulfonate-Functionalized Magnetic Solid-Extraction Adsorbent for Sensitive Detection of 30 Synthetic Cannabinoids in Wastewater

Published: Jan. 1, 2025

Language: Английский

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Indigenous data protection in wastewater surveillance: balancing public health monitoring with privacy rights

Genomic psychiatry :, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 6

Published: Feb. 25, 2025

Wastewater-based epidemiology (WBE) has revolutionized public health surveillance by enabling real-time monitoring of disease patterns across populations through analysis community wastewater. This innovative approach provides precise geographical tracking pathogen levels and spread detecting viral RNA bacterial DNA signatures. Beyond detection, wastewater reveals comprehensive data, including human genomic information biomarkers prescription medication substance use patterns. For Indigenous populations, whose communities often occupy distinct areas, this detailed biological data collection raises significant privacy ethical concerns, particularly given historical research exploitation. By examining international case studies, we analyze instances where traditional knowledge have been misused in psychiatric neuroscience contexts, highlighting violations informed consent principles, sovereignty rights, reinforcement harmful stereotypes. The current regulatory gap ethics necessitates the development specialized WBE protocols for communities. These guidelines must balance benefits with stringent protections authentic engagement governance rights recognition. framework supports both epidemiological advancement protection communities’ autonomy age surveillance.

Language: Английский

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Nationwide suspect screening of new psychoactive substances (NPSs) and other controlled substances in Taiwan wastewater using liquid chromatography–High resolution mass spectrometry (LC-HRMS)

Chemosphere, Journal Year: 2025, Volume and Issue: 375, P. 144227 - 144227

Published: Feb. 27, 2025

Language: Английский

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A case for global standardisation of genomics and wastewater-based epidemiology

The Lancet Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 101092 - 101092

Published: March 1, 2025

Language: Английский

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Innovative approaches to monitor illicit drug use and novel psychoactive substances in Tunisia using wastewater-based epidemiology

Annales de Toxicologie Analytique, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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