Bioorthogonal Cleavage Chemistry: Harnessing the Bond‐Break Reactions for Biomolecule Manipulations in Living Systems DOI Open Access
Yuchao Zhu,

Feng Lin,

Ziqi Liu

et al.

Chinese Journal of Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Comprehensive Summary The advancement of bioorthogonal cleavage reactions (BCRs) has expanded the scope chemistry toolkit, leading to a diverse array innovative biological applications. These include but are not limited precise spatial and temporal activation intracellular probes, prodrugs, proteins, glycans, nucleic acids. Herein, we summarize recent efforts by our group develop BCRs for manipulating functional molecules in living species meet various needs, along with future perspectives this exciting field. How do you get into specific field? Could please share some experiences readers? Chemists good at both forming breaking bonds. Back 2013, while field was continuously thriving, most researchers focused on "ligation" type reactions. Alternatively, I started wonder whether could "bond‐cleavage" reactions? We reviewed literature found that is indeed yet be developed. immediately encouraged my graduate students such began look potential applications new chemistry. soon developed series "cleavage" can triggered metals, small molecules, as well photocatalysis. then applied these activate proteins other biomolecules, allowing gain‐of‐function study their properties inside cells. Small molecule drugs also activated within tumor bed, which led safer more efficient anti‐cancer drugs. Over past decade, have built decaging toolbox generally applicable virtually any interest, persistently working broadening spectrum reaction types This created direction broad utilities life sciences medicine. supervise your students? encourage think independently collaborate widely. would delighted if ideas let me learn something. In addition experimental training, pay great attention cultivating students' logical thinking, English writing, presentation skills. For regular weekly meetings, two will give in‐depth presentations research projects, all members provide constructive discussions suggestions. What important personality scientific research? opinion, traits curiosity, perseverance, critical thinking. favorite journal(s)? ACS Chemical Biology . us advices improving Chinese Journal Chemistry? Consider organizing special issues focus emerging areas chemistry, attract high‐quality submissions increase journal's impact.

Language: Английский

Numerical simulation of radiation force synthesis by pulsed acoustic waves DOI
Shuhan Chen, Jia Zhou, Antoine Riaud

et al.

Physical Review Applied, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 2, 2025

Selective acoustic tweezers can focus the radiation force on a single particle to manipulate it without affecting its neighbors. Traditionally, this has required highly complex and specialized hardware, incompatible with high-throughput manipulation. In two-dimensional numerical study, we demonstrate that two pairs of orthogonal transducers, known for manipulating arrays particles, also achieve selective Specifically, show selectivity is possible by using sequences pulses iteratively construct combined potential focused solely target particle. locked icon Physics Subject Headings (PhySH)Acoustic wave phenomenaAcoustofluidicsNonlinear acousticsAcoustic tweezersMicrofluidic devicesFinite-element methodSurface waveUltrasound techniques

Language: Английский

Citations

2
Acoustic virtual 3D scaffold for direct-interacting tumor organoid–immune cell coculture systems DOI Creative Commons
Shan Han,

Maike Chen,

Shuang Zhao

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(47)

Published: Nov. 22, 2024

Three-dimensional (3D) cell culture has revolutionized life sciences, particularly in organoid technologies. Traditional bioscaffold materials, however, complicate the detachment of tumor organoids and hamper routine use organoid–immune cocultures. Here, we show an acoustic virtual 3D scaffold (AV-Scaf) method to achieve culture, enabling a direct-interacting coculture system. The self-organization process cells is facilitated by vortex field, which enables bioassembly ion channel activation. This approach can significantly enhance influx calcium ions, thereby accelerating intercellular interactions cellular assemblies. We established scaffold-free melanoma breast cancer using AV-Scaf cocultured with T cells. found that our system resulted high activation state cells, characterized notable up-regulation granzyme B (2.82 17.5%) interferon-γ (1.36 16%). offers efficient for studies, advancing research immunotherapy development.

Language: Английский

Citations

5
Hydrogel Fibers‐Based Biointerfacing DOI

Xingmei Chen,

Yinghui Feng, Pei Zhang

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

Abstract The unique 1D structure of fibers offers intriguing attributes, including a high length‐to‐diameter ratio, miniatured size, light‐weight, and flexibility, making them suitable for various biomedical applications, such as health monitoring, disease treatment, minimally invasive surgeries. However, traditional fiber devices, typically composed rigid, dry, non‐living materials, are intrinsically different from the soft, wet, living essence biological tissues, thereby posing grand challenges long‐term, reliable, seamless interfacing with systems. Hydrogel have recently emerged promising candidate, in light their similarity to tissues mechanical, chemical aspects, well distinct geometry. In this review, comprehensive overview recent progress hydrogel fibers‐based biointerfacing technology is provided. It thoroughly summarizes manufacturing strategy functional design, especially optical electron conductive performance, responsiveness triggers thermal, magnetic field ultrasonic wave, etc. Such attributes enable which also examined detail. Future potential directions, biosafety, long‐term reliability, sterilization, multi‐modalities integration intelligent therapeutic systems, raised. This review will serve valuable resource further advancement implementation next‐generation technology.

Language: Английский

Citations

4
Advances in precise cell manipulation DOI Creative Commons
Yuan Ma,

Zhenwei Liang,

Y. Chen

et al.

Droplet, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Research on cells and organ‐like tissues is critical in the fields of molecular biology, genetic analysis, proteomics tissue engineering, others. In recent years, advancements precise cell manipulation technologies have made positioning batch processing feasible. Various methods are used for recognition, positioning, manipulation, assembly, often introducing external such as electric, magnetic, acoustic, or optical into liquid environment to interact with cells, applying forces induce movement rearrangement. Alternatively, three‐dimensional (3D) bioprinting technology employed assembly. This review will comprehensively assess status, principles, advantages, disadvantages, prospects these technologies, covering single‐cell multicellular biological 3D printing techniques.

Language: Английский

Citations

0
Biomimetic Individual and Group Behaviors of Bubble‐Thrusted Tubular Micro Sonobots: Unveiling the Mechanism Behind Phenomena DOI Open Access
Ying Wei, Xiaolong Lu, Yuliang Chen

et al.

Advanced Engineering Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

To overcome the propulsion difficulties in low Reynolds number environment, natural species have developed their exclusive swimming strategies after 1000 years of evolution. Inspired by those fascinating creatures, diverse artificial microrobots are proposed to achieve distinctive biomimetic motions. However, lack cognition for mechanism hinders exploration multimode biomimicking microrobots, especially at high‐speed locomotion. Herein, behaviors micro sonobot featured with multiple‐layer tubular constructions and trapped microbubbles serving as powerful microengines reported. The observed speed achieves tens millimeters per second. Different from previously reported bubble‐loaded it is that primary Bjerknes forces originating nonhomogeneous acoustic field make a great contribution orientation. Along streaming secondary forces, including individual locomotion group aggregation emerged theoretically analyzed. motion sonobots offers marvelous potentials building multifunctional micro/nanosystems nanosurgery, lab‐on‐a‐chip biosystems, chemical biological engineering, environmental detoxification, etc.

Language: Английский

Citations

0
Innovative Platforms in Regenerative Medicine: Bridging Research and Clinical Solutions DOI Open Access
Sajjad Jalali,

Zeynab Fathi,

Sohameh Mohebbi

et al.

Jentashapir Journal of Cellular and Molecular Biology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 13, 2025

Context: Recent advances in induced pluripotent stem cells (iPSCs), CRISPR-Cas9 gene editing, nanotechnologies, and artificial intelligence have revolutionized regenerative medicine (RM) as a transformative field for tackling difficult medical problems. These breakthroughs promise specific treatments, proper restoration of tissue function, substantial improvements the quality life patients whose ailments cannot yet be cured. This review explores cutting-edge advancements RM platforms such cell therapy, 3D bioprinting, nanotechnology. The study also aims to shed light on challenges clinical translation policy implications, which are crucial fostering sustainable progressive discipline. Evidence Acquisition: manuscript draws research development application technologies. It synthesizes data cells, engineering, vitro organoid industry, (AI), nanotechnology that illustrate therapeutic potential. identify ethical, regulatory, practical hurdles translating from practice. Results: Breakthroughs those iPSC-derived organoids, nanostructured materials exhibit significant preclinical settings. Platforms organ-on-chip AI tools further enhance drug discovery treatment monitoring, while biomaterials scaffold-based approaches repair regeneration. Nevertheless, despite these advances, persist regarding scale-up, safety, ethical considerations. Conclusions: Innovations represent paradigm shift purely symptomatic treatments restorative therapies. Successful integration into practice will require multidisciplinary collaborative work, imposition rigorous safety protocols, enabling regulatory frameworks. Addressing would enable realize its true potential foundation 21st-century healthcare.

Language: Английский

Citations

0
Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound‐Phased Array DOI Creative Commons
Rick J. P. van Bergen, Bart Groenen,

Daniëlle C. A. Duffhues

et al.

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Acoustic patterning is a noncontact method to manipulate the spatial distribution of small particles using forces generated in an ultrasound standing wave field. The technique has found applications fields such as cell sorting, microfabrication, and tissue engineering. For engineering, acoustic enables remote manipulation, even clinical settings. Conventional axial strategies rely on reflector‐based or dual‐probe approaches, limiting their application controlled setups incompatible with vivo conditions. In contrast, single‐sided lateral exploiting transmit beamforming capabilities tunability transducer array, can bridge gap applications. first time, clinical‐phased array used acoustically pattern microscale both directions, dynamic control over shape orientation by adjusting electronic delays. data are validate numerical model designed predict particle displacement current future experiments. Finally, successfully applied living cells, demonstrating potential translation proof concept toward tissues. conclusion, arrays augmenting flexibility advancing for

Language: Английский

Citations

0
Microfluidic cell carriers for cultured meat DOI
Yue Zhang,

Guanglin Wu,

Xiaoying Hu

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144149 - 144149

Published: April 1, 2025

Language: Английский

Citations

0
Penalization and deep learning algorithms in Holographic Direct Sound Printing to improve print uniformity DOI
Mahdi Derayatifar, Mohsen Habibi,

Rama Bhat

et al.

Additive manufacturing, Journal Year: 2025, Volume and Issue: unknown, P. 104782 - 104782

Published: April 1, 2025

Language: Английский

Citations

0
A critical review on advances and challenges of bioprinted cardiac patches DOI
Xiaoqing Zhang, Guangtao Zhao,

Tianyi Ma

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 189, P. 1 - 24

Published: Oct. 5, 2024

Language: Английский

Citations

3