Spatiotemporal dissection of collective cell migration and tissue morphogenesis during development by optogenetics DOI Creative Commons
Sijia Zhou, Bing Liu, Jiaying Liu

et al.

Seminars in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 166, P. 36 - 51

Published: Dec. 26, 2024

Collective cell migration and tissue morphogenesis play a variety of important roles in the development many species. Tissue often generates mechanical forces that alter shapes arrangements, resembling collective migration-like behaviors. Genetic methods have been widely used to study its like behavior, advancing our understanding these processes during development. However, growing body research shows is not simple behavior but combined with other cellular processes. In addition, different surrounding environments can also influence migrating cells, further complicating Due complexity developmental tissues, traditional genetic approaches encounter challenges limitations. Thus, some spatiotemporal control become urgent dissecting Optogenetics method combines optics genetics, providing perfect strategy for spatiotemporally controlling corresponding protein activity subcellular, or levels. this review, we introduce basic mechanisms underlying optogenetic tools. Then, demonstrate how applied vivo dissect Additionally, describe promising field. Together, review will guide facilitate future studies by optogenetics.

Language: Английский

How does the tubulin code facilitate directed cell migration? DOI Creative Commons

Miguel Marques Simoes-da-Silva,

Marin Barišić

Biochemical Society Transactions, Journal Year: 2025, Volume and Issue: 53(01)

Published: Feb. 21, 2025

Besides being a component of the cytoskeleton that provides structural integrity to cell, microtubules also serve as tracks for intracellular transport. As building units mitotic spindle, distribute chromosomes during cell division. By distributing organelles, vesicles, and proteins, they play pivotal role in diverse cellular processes, including migration, which reorganize facilitate polarization. Structurally, are built up α/β-tubulin dimers, consist various tubulin isotypes undergo multiple post-translational modifications (PTMs). These PTMs allow differentiate into functional subsets, influencing associated processes. This text explores current understanding roles particularly detyrosination acetylation, their implications human diseases.

Language: Английский

Citations

0
CCSer2 gates dynein activity at the cell periphery DOI
Juliana Zang, Daytan Gibson, Ann-Marie Zheng

et al.

The Journal of Cell Biology, Journal Year: 2025, Volume and Issue: 224(6)

Published: April 22, 2025

Cytoplasmic dynein-1 (dynein) is a microtubule-associated, minus end–directed motor that traffics hundreds of different cargos. Dynein must discriminate between cargos and traffic them at the appropriate time from correct cellular region. How dynein’s trafficking activity regulated in or space remains poorly understood. Here, we identify CCSer2 as first known protein to gate dynein spatial dimension. promotes migration developing zebrafish primordium cells, macrophages, cultured human cells by facilitating are acted on peripherally localized dynein. Our data suggest disfavors interaction its regulator Ndel1 cell edge, resulting activation. These findings support model where specificity achieved localization proteins trigger Ndel1’s release We propose defines broader class activate distinct microenvironments via regulating Ndel1–dynein interaction.

Language: Английский

Citations

0
Mechanisms and Therapeutic Strategies for Minority Cell‐Induced Paclitaxel Resistance and Tumor Progression Mediated by Mechanical Forces DOI Creative Commons
Xueyan Feng, Di Zhang, Guoxun Wang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract Chemotherapy remains a prevalent strategy in cancer therapy; however, the emergence of drug resistance poses considerable challenge to its efficacy. Most arises from accumulation genetic mutations minority resistant cells. The mechanisms underlying and progression these minority‐resistant cells (MRCs) remain poorly understood. This study employs force‐induced remnant magnetization spectroscopy (FIRMS) alongside various biological investigations reveal mechanical pathways for MRCs fostering tumor progression. findings show that Paclitaxel‐resistant have enhanced properties. These can transmit high‐intensity forces surrounding sensitive (SCs) through force transducer, Merlin. transmission facilitates assimilation SCs, subsequently strengthening contraction adhesion process is termed “mechano‐assimilation,” which accelerates development Interestingly, disturbances reductions mechano‐assimilation within tumors restore sensitivity Paclitaxel both vitro vivo. provides preliminary evidence highlighting contribution malignancy, mediated interactions. It also establishes foundation future research focused on integrating factors into innovative therapies.

Language: Английский

Citations

0
Mechanosensitive FHL2 tunes endothelial function DOI Creative Commons
Shailaja Seetharaman, John Devany, Ha Ram Kim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 17, 2024

Abstract Endothelial tissues are essential mechanosensors in the vasculature and facilitate adaptation to various blood flow-induced mechanical cues. Defects endothelial mechanoresponses can perturb tissue remodelling functions leading cardiovascular disease progression. In this context, precise mechanisms of contributing normal diseased functioning remain elusive. Here, we sought uncover how flow-mediated transcriptional regulation drives healthy atherosclerotic-prone tissues. Using bulk RNA sequencing, identify novel mechanosensitive genes response unidirectional flow (UF) athero-prone disturbed (DF). We find that transcription as well protein expression Four-and-a-half LIM 2 (FHL2) enriched DF both vitro vivo . then demonstrate exogenous FHL2 is necessary sufficient drive discontinuous adherens junction morphology increased permeability. This phenotype requires force-sensitive binding actin. turn, force-dependent localisation stress fibres promotes microtubule dynamics release RhoGEF, GEF-H1, activate Rho-ROCK pathway. Thus, unravelled a mechanochemical feedback wherein hypercontractility. misregulated mechanoresponse creates highly permeable tissues, depicting classic hallmarks atherosclerosis Overall, highlight crucial for force-sensitivity tuning multi-scale mechanoresponses.

Language: Английский

Citations

3
Spatiotemporal dissection of collective cell migration and tissue morphogenesis during development by optogenetics DOI Creative Commons
Sijia Zhou, Bing Liu, Jiaying Liu

et al.

Seminars in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 166, P. 36 - 51

Published: Dec. 26, 2024

Collective cell migration and tissue morphogenesis play a variety of important roles in the development many species. Tissue often generates mechanical forces that alter shapes arrangements, resembling collective migration-like behaviors. Genetic methods have been widely used to study its like behavior, advancing our understanding these processes during development. However, growing body research shows is not simple behavior but combined with other cellular processes. In addition, different surrounding environments can also influence migrating cells, further complicating Due complexity developmental tissues, traditional genetic approaches encounter challenges limitations. Thus, some spatiotemporal control become urgent dissecting Optogenetics method combines optics genetics, providing perfect strategy for spatiotemporally controlling corresponding protein activity subcellular, or levels. this review, we introduce basic mechanisms underlying optogenetic tools. Then, demonstrate how applied vivo dissect Additionally, describe promising field. Together, review will guide facilitate future studies by optogenetics.

Language: Английский

Citations

0